1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
36
37
38
39
40
41
42
43
44
45
46
47
48
49
50
51
52
53
54
55
56
57
58
59
60
61
62
63
64
65
66
67
68
69
70
71
72
73
74
75
76
77
78
79
80
81
82
83
84
85
86
87
88
89
90
91
92
93
94
95
96
97
98
99
100
101
102
103
104
105
106
107
108
109
110
111
112
113
114
115
|
import argparse
import time
import arvados
import arvados.collection
import json
import logging
import magic
from pathlib import Path
import urllib.request
import socket
import getpass
import sys
sys.path.insert(0,'.')
from bh20sequploader.qc_metadata import qc_metadata
from bh20sequploader.qc_fasta import qc_fasta
logging.basicConfig(level=logging.DEBUG)
log = logging.getLogger(__name__ )
log.debug("Entering sequence uploader")
ARVADOS_API_HOST='lugli.arvadosapi.com'
ARVADOS_API_TOKEN='2fbebpmbo3rw3x05ueu2i6nx70zhrsb1p22ycu3ry34m4x4462'
UPLOAD_PROJECT='lugli-j7d0g-n5clictpuvwk8aa'
def qa_stuff(metadata, sequence_p1, sequence_p2):
try:
log.debug("Checking metadata")
if not qc_metadata(metadata.name):
log.warning("Failed metadata qc")
exit(1)
except ValueError as e:
log.debug(e)
log.debug("Failed metadata qc")
print(e)
exit(1)
target = []
try:
log.debug("Checking FASTA/FASTQ QC")
target.append(qc_fasta(sequence_p1))
if sequence_p2:
target.append(qc_fasta(sequence_p2))
target[0] = ("reads_1."+target[0][0][6:], target[0][1])
target[1] = ("reads_2."+target[1][0][6:], target[0][1])
except ValueError as e:
log.debug(e)
log.debug("Failed FASTA qc")
print(e)
exit(1)
return target
def upload_sequence(col, target, sequence):
with col.open(target[0], "wb") as f:
r = sequence.read(65536)
while r:
f.write(r)
r = sequence.read(65536)
def main():
parser = argparse.ArgumentParser(description='Upload SARS-CoV-19 sequences for analysis')
parser.add_argument('metadata', type=argparse.FileType('r'), help='sequence metadata json')
parser.add_argument('sequence_p1', type=argparse.FileType('rb'), help='sequence FASTA/FASTQ')
parser.add_argument('sequence_p2', type=argparse.FileType('rb'), default=None, help='sequence FASTQ pair')
parser.add_argument("--validate", action="store_true", help="Dry run, validate only")
args = parser.parse_args()
api = arvados.api(host=ARVADOS_API_HOST, token=ARVADOS_API_TOKEN, insecure=True)
target = qa_stuff(args.metadata, args.sequence_p1, args.sequence_p2)
seqlabel = target[0][1]
if args.validate:
print("Valid")
exit(0)
col = arvados.collection.Collection(api_client=api)
upload_sequence(col, target[0], args.sequence_p1)
if args.sequence_p2:
upload_sequence(col, target[1], args.sequence_p2)
print("Reading metadata")
with col.open("metadata.yaml", "w") as f:
r = args.metadata.read(65536)
print(r[0:20])
while r:
f.write(r)
r = args.metadata.read(65536)
external_ip = urllib.request.urlopen('https://ident.me').read().decode('utf8')
try:
username = getpass.getuser()
except KeyError:
username = "unknown"
properties = {
"sequence_label": seqlabel,
"upload_app": "bh20-seq-uploader",
"upload_ip": external_ip,
"upload_user": "%s@%s" % (username, socket.gethostname())
}
col.save_new(owner_uuid=UPLOAD_PROJECT, name="%s uploaded by %s from %s" %
(seqlabel, properties['upload_user'], properties['upload_ip']),
properties=properties, ensure_unique_name=True)
print("Saved to %s" % col.manifest_locator())
print("Done")
if __name__ == "__main__":
main()
|
