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import argparse
import arvados
import arvados.collection
import time
import subprocess
import tempfile
import json
import logging
import ruamel.yaml
from bh20sequploader.qc_metadata import qc_metadata
from bh20sequploader.qc_fasta import qc_fasta
import pkg_resources
from schema_salad.sourceline import add_lc_filename
logging.basicConfig(format="[%(asctime)s] %(levelname)s %(message)s", datefmt="%Y-%m-%d %H:%M:%S",
level=logging.INFO)
logging.getLogger("googleapiclient.discovery").setLevel(logging.WARN)
def validate_upload(api, collection, validated_project,
fastq_project, fastq_workflow_uuid):
col = arvados.collection.Collection(collection["uuid"])
# validate the collection here. Check metadata, etc.
valid = True
if "metadata.yaml" not in col:
logging.warn("Upload '%s' missing metadata.yaml", collection["name"])
valid = False
else:
try:
metadata_content = ruamel.yaml.round_trip_load(col.open("metadata.yaml"))
metadata_content["id"] = "http://arvados.org/keep:%s/metadata.yaml" % collection["portable_data_hash"]
add_lc_filename(metadata_content, metadata_content["id"])
valid = qc_metadata(metadata_content) and valid
except Exception as e:
logging.warn(e)
valid = False
if not valid:
logging.warn("Failed metadata qc")
if valid:
tgt = None
for n in ("sequence.fasta", "reads.fastq"):
if n not in col:
continue
with col.open(n) as qf:
tgt = qc_fasta(qf)
if tgt != n:
logging.info("Expected %s but magic says it should be %s", n, tgt)
valid = False
elif tgt == "reads.fastq":
start_fastq_to_fasta(api, collection, fastq_project, fastq_workflow_uuid)
return False
if tgt is None:
valid = False
logging.warn("Upload '%s' does not contain sequence.fasta or reads.fastq", collection["name"])
dup = api.collections().list(filters=[["owner_uuid", "=", validated_project],
["portable_data_hash", "=", col.portable_data_hash()]]).execute()
if dup["items"]:
# This exact collection has been uploaded before.
valid = False
logging.warn("Upload '%s' is duplicate" % collection["name"])
if valid:
logging.info("Added '%s' to validated sequences" % collection["name"])
# Move it to the "validated" project to be included in the next analysis
api.collections().update(uuid=collection["uuid"], body={
"owner_uuid": validated_project,
"name": "%s (%s)" % (collection["name"], time.asctime(time.gmtime()))}).execute()
else:
pass
# It is invalid, delete it.
#logging.warn("Deleting '%s'" % collection["name"])
#api.collections().delete(uuid=collection["uuid"]).execute()
return valid
def run_workflow(api, parent_project, workflow_uuid, name, inputobj):
project = api.groups().create(body={
"group_class": "project",
"name": name,
"owner_uuid": parent_project,
}, ensure_unique_name=True).execute()
with tempfile.NamedTemporaryFile() as tmp:
tmp.write(json.dumps(inputobj, indent=2).encode('utf-8'))
tmp.flush()
cmd = ["arvados-cwl-runner",
"--submit",
"--no-wait",
"--project-uuid=%s" % project["uuid"],
"arvwf:%s" % workflow_uuid,
tmp.name]
logging.info("Running %s" % ' '.join(cmd))
comp = subprocess.run(cmd, capture_output=True)
if comp.returncode != 0:
logging.error(comp.stderr.decode('utf-8'))
return project
def start_fastq_to_fasta(api, collection,
analysis_project,
fastq_workflow_uuid):
newproject = run_workflow(api, analysis_project, fastq_workflow_uuid, "FASTQ to FASTA", {
"fastq_forward": {
"class": "File",
"location": "keep:%s/reads.fastq" % collection["portable_data_hash"]
},
"metadata": {
"class": "File",
"location": "keep:%s/metadata.yaml" % collection["portable_data_hash"]
},
"ref_fasta": {
"class": "File",
"location": "keep:ffef6a3b77e5e04f8f62a7b6f67264d1+556/SARS-CoV2-NC_045512.2.fasta"
}
})
api.collections().update(uuid=collection["uuid"],
body={"owner_uuid": newproject["uuid"]}).execute()
def start_pangenome_analysis(api,
analysis_project,
pangenome_workflow_uuid,
validated_project,
schema_ref,
exclude_list):
validated = arvados.util.list_all(api.collections().list, filters=[["owner_uuid", "=", validated_project]])
inputobj = {
"inputReads": [],
"metadata": [],
"subjects": [],
"metadataSchema": {
"class": "File",
"location": schema_ref
},
"exclude": {
"class": "File",
"location": exclude_list
}
}
validated.sort(key=lambda v: v["portable_data_hash"])
for v in validated:
inputobj["inputReads"].append({
"class": "File",
"location": "keep:%s/sequence.fasta" % v["portable_data_hash"]
})
inputobj["metadata"].append({
"class": "File",
"location": "keep:%s/metadata.yaml" % v["portable_data_hash"]
})
inputobj["subjects"].append("http://collections.lugli.arvadosapi.com/c=%s/sequence.fasta" % v["portable_data_hash"])
run_workflow(api, analysis_project, pangenome_workflow_uuid, "Pangenome analysis", inputobj)
def get_workflow_output_from_project(api, uuid):
cr = api.container_requests().list(filters=[['owner_uuid', '=', uuid],
["requesting_container_uuid", "=", None]]).execute()
if cr["items"] and cr["items"][0]["output_uuid"]:
return cr["items"][0]
else:
return None
def copy_most_recent_result(api, analysis_project, latest_result_uuid):
most_recent_analysis = api.groups().list(filters=[['owner_uuid', '=', analysis_project]],
order="created_at desc", limit=1).execute()
for m in most_recent_analysis["items"]:
wf = get_workflow_output_from_project(api, m["uuid"])
if wf:
src = api.collections().get(uuid=wf["output_uuid"]).execute()
dst = api.collections().get(uuid=latest_result_uuid).execute()
if src["portable_data_hash"] != dst["portable_data_hash"]:
logging.info("Copying latest result from '%s' to %s", m["name"], latest_result_uuid)
api.collections().update(uuid=latest_result_uuid,
body={"manifest_text": src["manifest_text"],
"description": "Result from %s %s" % (m["name"], wf["uuid"])}).execute()
break
def move_fastq_to_fasta_results(api, analysis_project, uploader_project):
projects = api.groups().list(filters=[['owner_uuid', '=', analysis_project],
["properties.moved_output", "!=", True]],
order="created_at desc",).execute()
for p in projects["items"]:
wf = get_workflow_output_from_project(api, p["uuid"])
if wf:
logging.info("Moving completed fastq2fasta result %s back to uploader project", wf["output_uuid"])
api.collections().update(uuid=wf["output_uuid"],
body={"owner_uuid": uploader_project}).execute()
p["properties"]["moved_output"] = True
api.groups().update(uuid=p["uuid"], body={"properties": p["properties"]}).execute()
def upload_schema(api, workflow_def_project):
schema_resource = pkg_resources.resource_stream('bh20sequploader.qc_metadata', "bh20seq-schema.yml")
c = arvados.collection.Collection()
with c.open("schema.yml", "wb") as f:
f.write(schema_resource.read())
pdh = c.portable_data_hash()
wd = api.collections().list(filters=[["owner_uuid", "=", workflow_def_project],
["portable_data_hash", "=", pdh]]).execute()
if len(wd["items"]) == 0:
c.save_new(owner_uuid=workflow_def_project, name="Metadata schema", ensure_unique_name=True)
return "keep:%s/schema.yml" % pdh
def main():
parser = argparse.ArgumentParser(description='Analyze collections uploaded to a project')
parser.add_argument('--uploader-project', type=str, default='lugli-j7d0g-n5clictpuvwk8aa', help='')
parser.add_argument('--pangenome-analysis-project', type=str, default='lugli-j7d0g-y4k4uswcqi3ku56', help='')
parser.add_argument('--fastq-project', type=str, default='lugli-j7d0g-xcjxp4oox2u1w8u', help='')
parser.add_argument('--validated-project', type=str, default='lugli-j7d0g-5ct8p1i1wrgyjvp', help='')
parser.add_argument('--workflow-def-project', type=str, default='lugli-j7d0g-5hswinmpyho8dju', help='')
parser.add_argument('--pangenome-workflow-uuid', type=str, default='lugli-7fd4e-mqfu9y3ofnpnho1', help='')
parser.add_argument('--fastq-workflow-uuid', type=str, default='lugli-7fd4e-2zp9q4jo5xpif9y', help='')
parser.add_argument('--exclude-list', type=str, default='keep:lugli-4zz18-tzzhcm6hrf8ci8d/exclude.txt', help='')
parser.add_argument('--latest-result-collection', type=str, default='lugli-4zz18-z513nlpqm03hpca', help='')
parser.add_argument('--kickoff', action="store_true")
args = parser.parse_args()
api = arvados.api()
schema_ref = upload_schema(api, args.workflow_def_project)
if args.kickoff:
logging.info("Starting a single analysis run")
start_pangenome_analysis(api,
args.pangenome_analysis_project,
args.pangenome_workflow_uuid,
args.validated_project,
schema_ref,
args.exclude_list)
return
logging.info("Starting up, monitoring %s for uploads" % (args.uploader_project))
while True:
move_fastq_to_fasta_results(api, args.fastq_project, args.uploader_project)
new_collections = api.collections().list(filters=[['owner_uuid', '=', args.uploader_project]]).execute()
at_least_one_new_valid_seq = False
for c in new_collections["items"]:
at_least_one_new_valid_seq = validate_upload(api, c,
args.validated_project,
args.fastq_project,
args.fastq_workflow_uuid) or at_least_one_new_valid_seq
if at_least_one_new_valid_seq:
start_pangenome_analysis(api,
args.pangenome_analysis_project,
args.pangenome_workflow_uuid,
args.validated_project,
schema_ref,
args.exclude_list)
copy_most_recent_result(api,
args.pangenome_analysis_project,
args.latest_result_collection)
time.sleep(15)
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