diff options
Diffstat (limited to 'workflows/pull-data/genbank/update-from-genbank.py')
-rwxr-xr-x | workflows/pull-data/genbank/update-from-genbank.py | 114 |
1 files changed, 0 insertions, 114 deletions
diff --git a/workflows/pull-data/genbank/update-from-genbank.py b/workflows/pull-data/genbank/update-from-genbank.py deleted file mode 100755 index e62a611..0000000 --- a/workflows/pull-data/genbank/update-from-genbank.py +++ /dev/null @@ -1,114 +0,0 @@ -#!/usr/bin/env python3 -# -# - bulk download genbank data and matadata, preparing the FASTA and -# the YAML files -# -# update-from-genbank.py --max 10 --skip ids.txt --outdir ~/tmp/genbank -# -# See directory .guix-run and README.md - -BATCH_SIZE=5000 - -import argparse -parser = argparse.ArgumentParser() -parser.add_argument('--max', type=int, help='Max queries', required=False) -parser.add_argument('--skip', type=str, help='File with ids to skip, 1 id per line', required=False) -parser.add_argument('--outdir', type=str, help='Output directory', required=True) -args = parser.parse_args() - -from Bio import Entrez -Entrez.email = 'another_email@gmail.com' # FIXME - -import xml.etree.ElementTree as ET -import json -import os -import requests - -from datetime import date, datetime -from dateutil.parser import parse - -import sys -# sys.path.append('../') -from utils import is_integer, chunks, check_and_get_ontology_dictionaries - -num_ids_for_request = 100 # batch calls -min_acceptable_collection_date = datetime(2019, 12, 1) - -outdir = args.outdir - -today_date = date.today().strftime("%Y.%m.%d") -path_ncbi_virus_accession = 'sequences.{}.acc'.format(today_date) - -if not os.path.exists(outdir): - raise Exception(f"Output directory {outdir} does not exist!") - -skip = set() -if args.skip: - with open(args.skip) as f: - content = f.readlines() - for line in content: - skip.add(line.strip()) - -print(f"Skip size is {len(skip)}",file=sys.stderr) - -# ---------------------------------------------------------------------- -""" -Download section for genbank XML -""" - -# Try to search several strings -TERMS = ['SARS-CoV-2', 'SARS-CoV2', 'SARS CoV2', 'SARSCoV2', 'txid2697049[Organism]'] -# Remove mRNAs, ncRNAs, Proteins, and predicted models (more information here: https://en.wikipedia.org/wiki/RefSeq) starting with -PREFIX = ['NM', 'NR', 'NP', 'XM', 'XR', 'XP', 'WP'] - -ids = set() -for term in TERMS: - num_read = BATCH_SIZE - retstart = 0 - while num_read == BATCH_SIZE: - record = Entrez.read( - Entrez.esearch(db='nuccore', term=term, idtype='acc', - retstart=retstart, retmax=BATCH_SIZE) - ) - idlist = record['IdList'] - new_ids = set(idlist) - num_read = len(new_ids) - print(num_read,":",idlist[0],file=sys.stderr) - retstart += num_read - new_ids.difference_update(skip) # remove skip ids - new_ids = set([id for id in new_ids if id[:2] not in PREFIX]) - ids.update(new_ids) # add to total set - print(f"Term: {term} --> #{len(new_ids)} new IDs ---> Total unique IDs #{len(ids)})",file=sys.stderr) - if args.max and len(ids) > args.max: - print(f"Stopping past #{args.max} items",file=sys.stderr) - break - -for id in ids: - print(id) - -sys.exit(2) - -with open(path_ncbi_virus_accession) as f: - tmp_list = [line.strip('\n') for line in f] - -new_ids_set = set(tmp_list) -if len(accession_to_consider_set) > 0: - new_ids_set = new_ids_set.intersection(accession_to_consider_set) - -new_ids = len(new_ids_set.difference(id_set)) -id_set.update(new_ids_set) - -print('DB: NCBI Virus', today_date, '-->', new_ids, 'new IDs from', len(tmp_list), '---> Total unique IDs:', len(id_set)) - -id_set = id_set.difference(accession_to_ignore_set) -print('There are {} missing IDs to download.'.format(len(id_set))) - -os.makedirs(outdir) -for i, id_x_list in enumerate(chunks(list(id_set), num_ids_for_request)): - path_metadata_xxx_xml = os.path.join(outdir, 'metadata_{}.xml'.format(i)) - print('Requesting {} ids --> {}'.format(len(id_x_list), path_metadata_xxx_xml)) - - with open(path_metadata_xxx_xml, 'w') as fw: - fw.write( - Entrez.efetch(db='nuccore', id=id_x_list, retmode='xml').read() - ) |