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| -rw-r--r-- | doc/blog/using-covid-19-pubseq-part1.html | 64 | ||||
| -rw-r--r-- | doc/blog/using-covid-19-pubseq-part1.org | 23 | ||||
| -rw-r--r-- | doc/blog/using-covid-19-pubseq-part3.html | 245 | ||||
| -rw-r--r-- | doc/blog/using-covid-19-pubseq-part3.org | 123 |
4 files changed, 382 insertions, 73 deletions
diff --git a/doc/blog/using-covid-19-pubseq-part1.html b/doc/blog/using-covid-19-pubseq-part1.html index 5e52b82..1959fac 100644 --- a/doc/blog/using-covid-19-pubseq-part1.html +++ b/doc/blog/using-covid-19-pubseq-part1.html @@ -3,7 +3,7 @@ "http://www.w3.org/TR/xhtml1/DTD/xhtml1-strict.dtd"> <html xmlns="http://www.w3.org/1999/xhtml" lang="en" xml:lang="en"> <head> -<!-- 2020-05-29 Fri 10:12 --> +<!-- 2020-05-29 Fri 12:06 --> <meta http-equiv="Content-Type" content="text/html;charset=utf-8" /> <meta name="viewport" content="width=device-width, initial-scale=1" /> <title>COVID-19 PubSeq (part 1)</title> @@ -242,40 +242,26 @@ for the JavaScript code in this tag. </script> </head> <body> -<div id="org-div-home-and-up"> - <a accesskey="h" href=""> UP </a> - | - <a accesskey="H" href="http://covid19.genenetwork.org"> HOME </a> -</div><div id="content"> +<div id="content"> <h1 class="title">COVID-19 PubSeq (part 1)</h1> <div id="table-of-contents"> <h2>Table of Contents</h2> <div id="text-table-of-contents"> <ul> -<li><a href="#org5e85b09">1. What does this mean?</a></li> -<li><a href="#org038e367">2. Fetch sequence data</a></li> -<li><a href="#org3ad046c">3. Predicates</a></li> -<li><a href="#orga4e7054">4. Fetch submitter info and other metadata</a></li> -<li><a href="#orgc50badd">5. Fetch all sequences from Washington state</a></li> -<li><a href="#orgbc80874">6. Discussion</a></li> -<li><a href="#orgce8eaf6">7. Acknowledgements</a></li> +<li><a href="#org9afe6ab">1. What does this mean?</a></li> +<li><a href="#orgf4bc3d4">2. Fetch sequence data</a></li> +<li><a href="#org9d7d482">3. Predicates</a></li> +<li><a href="#orgc6046bb">4. Fetch submitter info and other metadata</a></li> +<li><a href="#orgdcb216b">5. Fetch all sequences from Washington state</a></li> +<li><a href="#org7060f51">6. Discussion</a></li> +<li><a href="#orgdc51ccc">7. Acknowledgements</a></li> </ul> </div> </div> -<p> -As part of the COVID-19 Biohackathon 2020 we formed a working group -to create a COVID-19 Public Sequence Resource (COVID-19 PubSeq) for -Corona virus sequences. The general idea is to create a repository -that has a low barrier to entry for uploading sequence data using best -practices. I.e., data published with a creative commons 4.0 (CC-4.0) -license with metadata using state-of-the art standards and, perhaps -most importantly, providing standardised workflows that get triggered -on upload, so that results are immediately available in standardised -data formats. -</p> -<div id="outline-container-org5e85b09" class="outline-2"> -<h2 id="org5e85b09"><span class="section-number-2">1</span> What does this mean?</h2> + +<div id="outline-container-org9afe6ab" class="outline-2"> +<h2 id="org9afe6ab"><span class="section-number-2">1</span> What does this mean?</h2> <div class="outline-text-2" id="text-1"> <p> This means that when someone uploads a SARS-CoV-2 sequence using one @@ -328,8 +314,8 @@ initiative! </div> -<div id="outline-container-org038e367" class="outline-2"> -<h2 id="org038e367"><span class="section-number-2">2</span> Fetch sequence data</h2> +<div id="outline-container-orgf4bc3d4" class="outline-2"> +<h2 id="orgf4bc3d4"><span class="section-number-2">2</span> Fetch sequence data</h2> <div class="outline-text-2" id="text-2"> <p> The latest run of the pipeline can be viewed <a href="https://workbench.lugli.arvadosapi.com/collections/lugli-4zz18-z513nlpqm03hpca">here</a>. Each of these @@ -353,8 +339,8 @@ these identifiers throughout. </div> </div> -<div id="outline-container-org3ad046c" class="outline-2"> -<h2 id="org3ad046c"><span class="section-number-2">3</span> Predicates</h2> +<div id="outline-container-org9d7d482" class="outline-2"> +<h2 id="org9d7d482"><span class="section-number-2">3</span> Predicates</h2> <div class="outline-text-2" id="text-3"> <p> To explore an RDF dataset, the first query we can do is open and gets @@ -464,8 +450,8 @@ Now we got this far, lets <a href="http://sparql.genenetwork.org/sparql/?default </div> -<div id="outline-container-orga4e7054" class="outline-2"> -<h2 id="orga4e7054"><span class="section-number-2">4</span> Fetch submitter info and other metadata</h2> +<div id="outline-container-orgc6046bb" class="outline-2"> +<h2 id="orgc6046bb"><span class="section-number-2">4</span> Fetch submitter info and other metadata</h2> <div class="outline-text-2" id="text-4"> <p> To get dataests with submitters we can do the above @@ -575,8 +561,8 @@ to view/query the database. </div> </div> -<div id="outline-container-orgc50badd" class="outline-2"> -<h2 id="orgc50badd"><span class="section-number-2">5</span> Fetch all sequences from Washington state</h2> +<div id="outline-container-orgdcb216b" class="outline-2"> +<h2 id="orgdcb216b"><span class="section-number-2">5</span> Fetch all sequences from Washington state</h2> <div class="outline-text-2" id="text-5"> <p> Now we know how to get at the origin we can do it the other way round @@ -603,8 +589,8 @@ half of the set coming out of GenBank. </div> </div> -<div id="outline-container-orgbc80874" class="outline-2"> -<h2 id="orgbc80874"><span class="section-number-2">6</span> Discussion</h2> +<div id="outline-container-org7060f51" class="outline-2"> +<h2 id="org7060f51"><span class="section-number-2">6</span> Discussion</h2> <div class="outline-text-2" id="text-6"> <p> The public sequence uploader collects sequences, raw data and @@ -615,8 +601,8 @@ referenced in publications and origins are citeable. </div> </div> -<div id="outline-container-orgce8eaf6" class="outline-2"> -<h2 id="orgce8eaf6"><span class="section-number-2">7</span> Acknowledgements</h2> +<div id="outline-container-orgdc51ccc" class="outline-2"> +<h2 id="orgdc51ccc"><span class="section-number-2">7</span> Acknowledgements</h2> <div class="outline-text-2" id="text-7"> <p> The overall effort was due to magnificent freely donated input by a @@ -631,7 +617,7 @@ Garrison this initiative would not have existed! </div> </div> <div id="postamble" class="status"> -<hr><small>Created by <a href="http://thebird.nl/">Pjotr Prins</a> (pjotr.public768 at thebird 'dot' nl) using Emacs org-mode and a healthy dose of Lisp!<br />Modified 2020-05-29 Fri 10:12</small>. +<hr><small>Created by <a href="http://thebird.nl/">Pjotr Prins</a> (pjotr.public768 at thebird 'dot' nl) using Emacs org-mode and a healthy dose of Lisp!<br />Modified 2020-05-29 Fri 12:06</small>. </div> </body> </html> diff --git a/doc/blog/using-covid-19-pubseq-part1.org b/doc/blog/using-covid-19-pubseq-part1.org index 5a749d6..0fd5589 100644 --- a/doc/blog/using-covid-19-pubseq-part1.org +++ b/doc/blog/using-covid-19-pubseq-part1.org @@ -5,18 +5,8 @@ # C-c C-t task rotate # RSS_IMAGE_URL: http://xxxx.xxxx.free.fr/rss_icon.png -#+HTML_LINK_HOME: http://covid19.genenetwork.org #+HTML_HEAD: <link rel="Blog stylesheet" type="text/css" href="blog.css" /> -As part of the COVID-19 Biohackathon 2020 we formed a working group -to create a COVID-19 Public Sequence Resource (COVID-19 PubSeq) for -Corona virus sequences. The general idea is to create a repository -that has a low barrier to entry for uploading sequence data using best -practices. I.e., data published with a creative commons 4.0 (CC-4.0) -license with metadata using state-of-the art standards and, perhaps -most importantly, providing standardised workflows that get triggered -on upload, so that results are immediately available in standardised -data formats. * Table of Contents :TOC:noexport: - [[#what-does-this-mean][What does this mean?]] @@ -261,7 +251,6 @@ Now we know how to get at the origin we can do it the other way round and fetch all sequences referring to Washington state #+begin_src sql - select ?seq ?sample { ?seq <http://biohackathon.org/bh20-seq-schema#MainSchema/sample> ?sample . @@ -272,6 +261,18 @@ select ?seq ?sample which lists 300 sequences originating from Washington state! Which is almost half of the set coming out of GenBank. +Likewise to list all sequences from Turkey we can find the wikidata +entity is [[https://www.wikidata.org/wiki/Q43][Q43]]: + +#+begin_src sql +select ?seq ?sample +{ + ?seq <http://biohackathon.org/bh20-seq-schema#MainSchema/sample> ?sample . + ?sample <http://purl.obolibrary.org/obo/GAZ_00000448> <http://www.wikidata.org/entity/Q43> +} +#+end_src + + * Discussion The public sequence uploader collects sequences, raw data and diff --git a/doc/blog/using-covid-19-pubseq-part3.html b/doc/blog/using-covid-19-pubseq-part3.html index 7903791..6838bc7 100644 --- a/doc/blog/using-covid-19-pubseq-part3.html +++ b/doc/blog/using-covid-19-pubseq-part3.html @@ -3,7 +3,7 @@ "http://www.w3.org/TR/xhtml1/DTD/xhtml1-strict.dtd"> <html xmlns="http://www.w3.org/1999/xhtml" lang="en" xml:lang="en"> <head> -<!-- 2020-05-29 Fri 10:00 --> +<!-- 2020-05-29 Fri 14:22 --> <meta http-equiv="Content-Type" content="text/html;charset=utf-8" /> <meta name="viewport" content="width=device-width, initial-scale=1" /> <title>COVID-19 PubSeq Uploading Data (part 3)</title> @@ -248,16 +248,42 @@ for the JavaScript code in this tag. <h2>Table of Contents</h2> <div id="text-table-of-contents"> <ul> -<li><a href="#orgbfd8594">1. Uploading Data</a></li> -<li><a href="#org3243122">2. Introduction</a></li> -<li><a href="#orgc7011c9">3. Step 1: Sequence</a></li> -<li><a href="#org83d22ff">4. Step 2: Metadata</a></li> +<li><a href="#orgb5456df">1. Uploading Data</a></li> +<li><a href="#org5b96fa9">2. Introduction</a></li> +<li><a href="#orga21edf3">3. Step 1: Upload sequence</a></li> +<li><a href="#orga03c092">4. Step 2: Add metadata</a> +<ul> +<li><a href="#org2ab94ef">4.1. Obligatory fields</a> +<ul> +<li><a href="#org9972a05">4.1.1. Sample ID (sample<sub>id</sub>)</a></li> +<li><a href="#orgf4992bb">4.1.2. Collection date</a></li> +<li><a href="#org2f55ae7">4.1.3. Collection location</a></li> +<li><a href="#orgb10db8a">4.1.4. Sequencing technology</a></li> +<li><a href="#orgf846ffe">4.1.5. Authors</a></li> +</ul> +</li> +<li><a href="#org2056637">4.2. Optional fields</a> +<ul> +<li><a href="#orgb2348b1">4.2.1. Host information</a></li> +<li><a href="#orgd963089">4.2.2. Collecting institution</a></li> +<li><a href="#org3257813">4.2.3. Specimen source</a></li> +<li><a href="#org8a596c8">4.2.4. Source database accession</a></li> +<li><a href="#orgd1f5c90">4.2.5. Strain name</a></li> +</ul> +</li> +</ul> +</li> +<li><a href="#orgb9edfdf">5. Step 3: Submit to COVID-19 PubSeq</a> +<ul> +<li><a href="#orgc929675">5.1. Trouble shooting</a></li> +</ul> +</li> </ul> </div> </div> -<div id="outline-container-orgbfd8594" class="outline-2"> -<h2 id="orgbfd8594"><span class="section-number-2">1</span> Uploading Data</h2> +<div id="outline-container-orgb5456df" class="outline-2"> +<h2 id="orgb5456df"><span class="section-number-2">1</span> Uploading Data</h2> <div class="outline-text-2" id="text-1"> <p> <i>Work in progress!</i> @@ -265,8 +291,8 @@ for the JavaScript code in this tag. </div> </div> -<div id="outline-container-org3243122" class="outline-2"> -<h2 id="org3243122"><span class="section-number-2">2</span> Introduction</h2> +<div id="outline-container-org5b96fa9" class="outline-2"> +<h2 id="org5b96fa9"><span class="section-number-2">2</span> Introduction</h2> <div class="outline-text-2" id="text-2"> <p> The COVID-19 PubSeq allows you to upload your SARS-Cov-2 strains to a @@ -276,27 +302,214 @@ upload. Read the <a href="./about">ABOUT</a> page for more information. </div> </div> -<div id="outline-container-orgc7011c9" class="outline-2"> -<h2 id="orgc7011c9"><span class="section-number-2">3</span> Step 1: Sequence</h2> +<div id="outline-container-orga21edf3" class="outline-2"> +<h2 id="orga21edf3"><span class="section-number-2">3</span> Step 1: Upload sequence</h2> <div class="outline-text-2" id="text-3"> <p> +To upload a sequence in the <a href="http://covid19.genenetwork.org/">web upload page</a> hit the browse button and +select the FASTA file on your local hard disk. +</p> + +<p> We start with an assembled or mapped sequence in FASTA format. The PubSeq uploader contains a <a href="https://github.com/arvados/bh20-seq-resource/blob/master/bh20sequploader/qc_fasta.py">QC step</a> which checks whether it is a likely SARS-CoV-2 sequence. While PubSeq deduplicates sequences and never -overwrites metadata it probably pays to check whether your data +overwrites metadata, you may still want to check whether your data already is in the system by querying some metadata as described in -<a href="./blog?id=using-covid-19-pubseq-part1">Query metadata with SPARQL</a>. +<a href="./blog?id=using-covid-19-pubseq-part1">Query metadata with SPARQL</a> or by simply downloading and checking one +of the files on the <a href="./download">download</a> page. We find GenBank <a href="https://www.ncbi.nlm.nih.gov/nuccore/MT536190">MT536190.1</a> has not +been included yet. A FASTA text file can be <a href="https://www.ncbi.nlm.nih.gov/nuccore/MT536190.1?report=fasta&log$=seqview&format=text">downloaded</a> to your local +disk and uploaded through our <a href="./">web upload page</a>. Make sure the file does +not include any HTML! +</p> + +<p> +Note: we currently only allow FASTA uploads. In the near future we'll +allow for uploading raw sequence files. This is important for creating +an improved pangenome. +</p> +</div> +</div> + +<div id="outline-container-orga03c092" class="outline-2"> +<h2 id="orga03c092"><span class="section-number-2">4</span> Step 2: Add metadata</h2> +<div class="outline-text-2" id="text-4"> +<p> +The <a href="./">web upload page</a> contains fields for adding metadata. Metadata is +not only important for attribution, is also important for +analysis. The metadata is available for queries, see <a href="./blog?id=using-covid-19-pubseq-part1">Query metadata +with SPARQL</a>, and can be used to annotate variations of the virus in +different ways. +</p> + +<p> +A number of fields are obligatory: sample id, date, location, +technology and authors. The others are optional, but it is valuable to +enter them when information is available. Metadata is defined in this +<a href="https://github.com/arvados/bh20-seq-resource/blob/master/bh20sequploader/bh20seq-schema.yml">schema</a>. From this schema we generate the input form. Note that +opitional fields have a question mark in the <code>type</code>. You can add +metadata yourself, btw, because this is a public resource! See also +<a href="./blog?id=using-covid-19-pubseq-part5">Modify metadata</a> for more information. +</p> + +<p> +To get more information about a field click on the question mark on +the web form. Here we add some extra information. +</p> +</div> + +<div id="outline-container-org2ab94ef" class="outline-3"> +<h3 id="org2ab94ef"><span class="section-number-3">4.1</span> Obligatory fields</h3> +<div class="outline-text-3" id="text-4-1"> +</div> +<div id="outline-container-org9972a05" class="outline-4"> +<h4 id="org9972a05"><span class="section-number-4">4.1.1</span> Sample ID (sample<sub>id</sub>)</h4> +<div class="outline-text-4" id="text-4-1-1"> +<p> +This is a string field that defines a unique sample identifier by the +submitter. In addition to sample<sub>id</sub> we also have host<sub>id</sub>, +provider<sub>sample</sub><sub>id</sub> and submitter<sub>sample</sub><sub>id</sub> where host is the host the +sample came from, provider sample is the institution sample id and +submitter is the submitting individual id. host<sub>id</sub> is important when +multiple sequences come from the same host. Make sure not to have +spaces in the sample<sub>id</sub>. +</p> + +<p> +Here we add the GenBank ID MT536190.1. +</p> +</div> +</div> + +<div id="outline-container-orgf4992bb" class="outline-4"> +<h4 id="orgf4992bb"><span class="section-number-4">4.1.2</span> Collection date</h4> +<div class="outline-text-4" id="text-4-1-2"> +<p> +Estimated collection date. The GenBank page says April 6, 2020. +</p> +</div> +</div> + +<div id="outline-container-org2f55ae7" class="outline-4"> +<h4 id="org2f55ae7"><span class="section-number-4">4.1.3</span> Collection location</h4> +<div class="outline-text-4" id="text-4-1-3"> +<p> +A search on wikidata says Los Angelos is +<a href="https://www.wikidata.org/entity/Q65">https://www.wikidata.org/entity/Q65</a> +</p> +</div> +</div> + +<div id="outline-container-orgb10db8a" class="outline-4"> +<h4 id="orgb10db8a"><span class="section-number-4">4.1.4</span> Sequencing technology</h4> +<div class="outline-text-4" id="text-4-1-4"> +<p> +GenBank entry says Illumina, so we can fill that in +</p> +</div> +</div> + +<div id="outline-container-orgf846ffe" class="outline-4"> +<h4 id="orgf846ffe"><span class="section-number-4">4.1.5</span> Authors</h4> +<div class="outline-text-4" id="text-4-1-5"> +<p> +GenBank entry says 'Lamers,S., Nolan,D.J., Rose,R., Cross,S., Moraga +Amador,D., Yang,T., Caruso,L., Navia,W., Von Borstel,L., Hui Zhou,X., +Freehan,A. and Garcia-Diaz,J.', so we can fill that in. +</p> +</div> +</div> +</div> + +<div id="outline-container-org2056637" class="outline-3"> +<h3 id="org2056637"><span class="section-number-3">4.2</span> Optional fields</h3> +<div class="outline-text-3" id="text-4-2"> +<p> +All other fields are optional. But let's see what we can add. +</p> +</div> + +<div id="outline-container-orgb2348b1" class="outline-4"> +<h4 id="orgb2348b1"><span class="section-number-4">4.2.1</span> Host information</h4> +<div class="outline-text-4" id="text-4-2-1"> +<p> +Sadly, not much is known about the host from GenBank. A little +sleuthing renders an interesting paper by some of the authors titled +<a href="https://www.medrxiv.org/content/10.1101/2020.04.24.20078691v1">SARS-CoV-2 is consistent across multiple samples and methodologies</a> +which dates after the sample, but has no reference other than that the +raw data came from the SRA database, so it probably does not describe +this particular sample. We don't know what this strain of SARS-Cov-2 +did to the person and what the person was like (say age group). +</p> +</div> +</div> + +<div id="outline-container-orgd963089" class="outline-4"> +<h4 id="orgd963089"><span class="section-number-4">4.2.2</span> Collecting institution</h4> +<div class="outline-text-4" id="text-4-2-2"> +<p> +We can fill that in. +</p> +</div> +</div> + +<div id="outline-container-org3257813" class="outline-4"> +<h4 id="org3257813"><span class="section-number-4">4.2.3</span> Specimen source</h4> +<div class="outline-text-4" id="text-4-2-3"> +<p> +We have that: nasopharyngeal swab </p> </div> </div> +<div id="outline-container-org8a596c8" class="outline-4"> +<h4 id="org8a596c8"><span class="section-number-4">4.2.4</span> Source database accession</h4> +<div class="outline-text-4" id="text-4-2-4"> +<p> +Genbank which is <a href="http://identifiers.org/insdc/MT536190.1#sequence">http://identifiers.org/insdc/MT536190.1#sequence</a>. +Note we plug in our own identifier MT536190.1. +</p> +</div> +</div> -<div id="outline-container-org83d22ff" class="outline-2"> -<h2 id="org83d22ff"><span class="section-number-2">4</span> Step 2: Metadata</h2> +<div id="outline-container-orgd1f5c90" class="outline-4"> +<h4 id="orgd1f5c90"><span class="section-number-4">4.2.5</span> Strain name</h4> +<div class="outline-text-4" id="text-4-2-5"> +<p> +SARS-CoV-2/human/USA/LA-BIE-070/2020 +</p> +</div> +</div> +</div> +</div> + +<div id="outline-container-orgb9edfdf" class="outline-2"> +<h2 id="orgb9edfdf"><span class="section-number-2">5</span> Step 3: Submit to COVID-19 PubSeq</h2> +<div class="outline-text-2" id="text-5"> +<p> +Once you have the sequence and the metadata together, hit +the 'Add to Pangenome' button. The data will be checked, +submitted and the workflows should kick in! +</p> +</div> + +<div id="outline-container-orgc929675" class="outline-3"> +<h3 id="orgc929675"><span class="section-number-3">5.1</span> Trouble shooting</h3> +<div class="outline-text-3" id="text-5-1"> +<p> +We got an error saying: {"stem": "<a href="http://www.wikidata.org/entity/">http://www.wikidata.org/entity/</a>",… +which means that our location field was not formed correctly! After +fixing it to look like <a href="http://www.wikidata.org/entity/Q65">http://www.wikidata.org/entity/Q65</a> (note http +instead on https and entity instead of wiki) the submission went +through. Reload the page (it won't empty the fields) to re-enable the +submit button. +</p> +</div> +</div> </div> </div> <div id="postamble" class="status"> -<hr><small>Created by <a href="http://thebird.nl/">Pjotr Prins</a> (pjotr.public768 at thebird 'dot' nl) using Emacs org-mode and a healthy dose of Lisp!<br />Modified 2020-05-29 Fri 10:00</small>. +<hr><small>Created by <a href="http://thebird.nl/">Pjotr Prins</a> (pjotr.public768 at thebird 'dot' nl) using Emacs org-mode and a healthy dose of Lisp!<br />Modified 2020-05-29 Fri 14:22</small>. </div> </body> </html> diff --git a/doc/blog/using-covid-19-pubseq-part3.org b/doc/blog/using-covid-19-pubseq-part3.org index 296bef6..ade902d 100644 --- a/doc/blog/using-covid-19-pubseq-part3.org +++ b/doc/blog/using-covid-19-pubseq-part3.org @@ -3,7 +3,6 @@ # C-c C-e h h publish # C-c ! insert date (use . for active agenda, C-u C-c ! for date, C-u C-c . for time) # C-c C-t task rotate -# RSS_IMAGE_URL: http://xxxx.xxxx.free.fr/rss_icon.png #+HTML_HEAD: <link rel="Blog stylesheet" type="text/css" href="blog.css" /> @@ -14,8 +13,12 @@ * Table of Contents :TOC:noexport: - [[#uploading-data][Uploading Data]] - [[#introduction][Introduction]] - - [[#step-1-sequence][Step 1: Sequence]] - - [[#step-2-metadata][Step 2: Metadata]] + - [[#step-1-upload-sequence][Step 1: Upload sequence]] + - [[#step-2-add-metadata][Step 2: Add metadata]] + - [[#obligatory-fields][Obligatory fields]] + - [[#optional-fields][Optional fields]] + - [[#step-3-submit-to-covid-19-pubseq][Step 3: Submit to COVID-19 PubSeq]] + - [[#trouble-shooting][Trouble shooting]] * Introduction @@ -23,14 +26,120 @@ The COVID-19 PubSeq allows you to upload your SARS-Cov-2 strains to a public resource for global comparisons. Compute it triggered on upload. Read the [[./about][ABOUT]] page for more information. -* Step 1: Sequence +* Step 1: Upload sequence + +To upload a sequence in the [[http://covid19.genenetwork.org/][web upload page]] hit the browse button and +select the FASTA file on your local hard disk. We start with an assembled or mapped sequence in FASTA format. The PubSeq uploader contains a [[https://github.com/arvados/bh20-seq-resource/blob/master/bh20sequploader/qc_fasta.py][QC step]] which checks whether it is a likely SARS-CoV-2 sequence. While PubSeq deduplicates sequences and never -overwrites metadata it probably pays to check whether your data +overwrites metadata, you may still want to check whether your data already is in the system by querying some metadata as described in -[[./blog?id=using-covid-19-pubseq-part1][Query metadata with SPARQL]]. +[[./blog?id=using-covid-19-pubseq-part1][Query metadata with SPARQL]] or by simply downloading and checking one +of the files on the [[./download][download]] page. We find GenBank [[https://www.ncbi.nlm.nih.gov/nuccore/MT536190][MT536190.1]] has not +been included yet. A FASTA text file can be [[https://www.ncbi.nlm.nih.gov/nuccore/MT536190.1?report=fasta&log$=seqview&format=text][downloaded]] to your local +disk and uploaded through our [[./][web upload page]]. Make sure the file does +not include any HTML! + +Note: we currently only allow FASTA uploads. In the near future we'll +allow for uploading raw sequence files. This is important for creating +an improved pangenome. + +* Step 2: Add metadata + +The [[./][web upload page]] contains fields for adding metadata. Metadata is +not only important for attribution, is also important for +analysis. The metadata is available for queries, see [[./blog?id=using-covid-19-pubseq-part1][Query metadata +with SPARQL]], and can be used to annotate variations of the virus in +different ways. + +A number of fields are obligatory: sample id, date, location, +technology and authors. The others are optional, but it is valuable to +enter them when information is available. Metadata is defined in this +[[https://github.com/arvados/bh20-seq-resource/blob/master/bh20sequploader/bh20seq-schema.yml][schema]]. From this schema we generate the input form. Note that +opitional fields have a question mark in the ~type~. You can add +metadata yourself, btw, because this is a public resource! See also +[[./blog?id=using-covid-19-pubseq-part5][Modify metadata]] for more information. + +To get more information about a field click on the question mark on +the web form. Here we add some extra information. + +** Obligatory fields + +*** Sample ID (sample_id) + +This is a string field that defines a unique sample identifier by the +submitter. In addition to sample_id we also have host_id, +provider_sample_id and submitter_sample_id where host is the host the +sample came from, provider sample is the institution sample id and +submitter is the submitting individual id. host_id is important when +multiple sequences come from the same host. Make sure not to have +spaces in the sample_id. + +Here we add the GenBank ID MT536190.1. + +*** Collection date + +Estimated collection date. The GenBank page says April 6, 2020. + +*** Collection location + +A search on wikidata says Los Angelos is +https://www.wikidata.org/entity/Q65 + +*** Sequencing technology + +GenBank entry says Illumina, so we can fill that in + +*** Authors + +GenBank entry says 'Lamers,S., Nolan,D.J., Rose,R., Cross,S., Moraga +Amador,D., Yang,T., Caruso,L., Navia,W., Von Borstel,L., Hui Zhou,X., +Freehan,A. and Garcia-Diaz,J.', so we can fill that in. + +** Optional fields + +All other fields are optional. But let's see what we can add. + +*** Host information + +Sadly, not much is known about the host from GenBank. A little +sleuthing renders an interesting paper by some of the authors titled +[[https://www.medrxiv.org/content/10.1101/2020.04.24.20078691v1][SARS-CoV-2 is consistent across multiple samples and methodologies]] +which dates after the sample, but has no reference other than that the +raw data came from the SRA database, so it probably does not describe +this particular sample. We don't know what this strain of SARS-Cov-2 +did to the person and what the person was like (say age group). + +*** Collecting institution + +We can fill that in. + +*** Specimen source + +We have that: nasopharyngeal swab + +*** Source database accession + +Genbank which is http://identifiers.org/insdc/MT536190.1#sequence. +Note we plug in our own identifier MT536190.1. + +*** Strain name + +SARS-CoV-2/human/USA/LA-BIE-070/2020 + +* Step 3: Submit to COVID-19 PubSeq + +Once you have the sequence and the metadata together, hit +the 'Add to Pangenome' button. The data will be checked, +submitted and the workflows should kick in! +** Trouble shooting -* Step 2: Metadata +We got an error saying: {"stem": "http://www.wikidata.org/entity/",... +which means that our location field was not formed correctly! After +fixing it to look like http://www.wikidata.org/entity/Q65 (note http +instead on https and entity instead of wiki) the submission went +through. Reload the page (it won't empty the fields) to re-enable the +submit button. |