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diff --git a/doc/blog/using-covid-19-pubseq-part3.html b/doc/blog/using-covid-19-pubseq-part3.html index df4a286..718b10f 100644 --- a/doc/blog/using-covid-19-pubseq-part3.html +++ b/doc/blog/using-covid-19-pubseq-part3.html @@ -3,7 +3,7 @@ "http://www.w3.org/TR/xhtml1/DTD/xhtml1-strict.dtd"> <html xmlns="http://www.w3.org/1999/xhtml" lang="en" xml:lang="en"> <head> -<!-- 2020-05-30 Sat 18:12 --> +<!-- 2020-08-24 Mon 04:31 --> <meta http-equiv="Content-Type" content="text/html;charset=utf-8" /> <meta name="viewport" content="width=device-width, initial-scale=1" /> <title>COVID-19 PubSeq Uploading Data (part 3)</title> @@ -248,40 +248,40 @@ for the JavaScript code in this tag. <h2>Table of Contents</h2> <div id="text-table-of-contents"> <ul> -<li><a href="#org193669a">1. Uploading Data</a></li> -<li><a href="#orgc6b3a47">2. Step 1: Upload sequence</a></li> -<li><a href="#org9c08714">3. Step 2: Add metadata</a> +<li><a href="#orgdaec996">1. Uploading Data</a></li> +<li><a href="#org8472a05">2. Step 1: Upload sequence</a></li> +<li><a href="#org668a46d">3. Step 2: Add metadata</a> <ul> -<li><a href="#org4c2e907">3.1. Obligatory fields</a> +<li><a href="#orga044bef">3.1. Obligatory fields</a> <ul> -<li><a href="#orgdddcb2e">3.1.1. Sample ID (sample<sub>id</sub>)</a></li> -<li><a href="#orge9c2e76">3.1.2. Collection date</a></li> -<li><a href="#org62c55ce">3.1.3. Collection location</a></li> -<li><a href="#org460b377">3.1.4. Sequencing technology</a></li> -<li><a href="#org77b1e14">3.1.5. Authors</a></li> +<li><a href="#org8e17492">3.1.1. Sample ID (sample<sub>id</sub>)</a></li> +<li><a href="#orgd9805db">3.1.2. Collection date</a></li> +<li><a href="#org3bd4901">3.1.3. Collection location</a></li> +<li><a href="#org921de27">3.1.4. Sequencing technology</a></li> +<li><a href="#org39fa678">3.1.5. Authors</a></li> </ul> </li> -<li><a href="#org3cb346f">3.2. Optional fields</a> +<li><a href="#org5315804">3.2. Optional fields</a> <ul> -<li><a href="#orgb0cffbb">3.2.1. Host information</a></li> -<li><a href="#orgd2a43a6">3.2.2. Collecting institution</a></li> -<li><a href="#org8d5bcf7">3.2.3. Specimen source</a></li> -<li><a href="#org86b21b2">3.2.4. Source database accession</a></li> -<li><a href="#org771ea66">3.2.5. Strain name</a></li> +<li><a href="#orgf2b82d9">3.2.1. Host information</a></li> +<li><a href="#org8986ca7">3.2.2. Collecting institution</a></li> +<li><a href="#orge03eb0c">3.2.3. Specimen source</a></li> +<li><a href="#org6815a6e">3.2.4. Source database accession</a></li> +<li><a href="#org51b37e8">3.2.5. Strain name</a></li> </ul> </li> </ul> </li> -<li><a href="#org7d281f5">4. Step 3: Submit to COVID-19 PubSeq</a> +<li><a href="#org5778da6">4. Step 3: Submit to COVID-19 PubSeq</a> <ul> -<li><a href="#orgdf0f02d">4.1. Trouble shooting</a></li> +<li><a href="#orge803d65">4.1. Trouble shooting</a></li> </ul> </li> -<li><a href="#org29f8a92">5. Step 4: Check output</a></li> -<li><a href="#orgf493854">6. Bulk sequence uploader</a> +<li><a href="#org540cfdf">5. Step 4: Check output</a></li> +<li><a href="#org6c43ab3">6. Bulk sequence uploader</a> <ul> -<li><a href="#org37fadbc">6.1. Run the uploader (CLI)</a></li> -<li><a href="#org39adf09">6.2. Example: uploading bulk GenBank sequences</a></li> +<li><a href="#org99bb8b7">6.1. Run the uploader (CLI)</a></li> +<li><a href="#orga88593f">6.2. Example: uploading bulk GenBank sequences</a></li> </ul> </li> </ul> @@ -290,8 +290,8 @@ for the JavaScript code in this tag. -<div id="outline-container-org193669a" class="outline-2"> -<h2 id="org193669a"><span class="section-number-2">1</span> Uploading Data</h2> +<div id="outline-container-orgdaec996" class="outline-2"> +<h2 id="orgdaec996"><span class="section-number-2">1</span> Uploading Data</h2> <div class="outline-text-2" id="text-1"> <p> The COVID-19 PubSeq allows you to upload your SARS-Cov-2 strains to a @@ -301,8 +301,8 @@ gets triggered on upload. Read the <a href="./about">ABOUT</a> page for more inf </div> </div> -<div id="outline-container-orgc6b3a47" class="outline-2"> -<h2 id="orgc6b3a47"><span class="section-number-2">2</span> Step 1: Upload sequence</h2> +<div id="outline-container-org8472a05" class="outline-2"> +<h2 id="org8472a05"><span class="section-number-2">2</span> Step 1: Upload sequence</h2> <div class="outline-text-2" id="text-2"> <p> To upload a sequence in the <a href="http://covid19.genenetwork.org/">web upload page</a> hit the browse button and @@ -330,8 +330,8 @@ an improved pangenome. </div> </div> -<div id="outline-container-org9c08714" class="outline-2"> -<h2 id="org9c08714"><span class="section-number-2">3</span> Step 2: Add metadata</h2> +<div id="outline-container-org668a46d" class="outline-2"> +<h2 id="org668a46d"><span class="section-number-2">3</span> Step 2: Add metadata</h2> <div class="outline-text-2" id="text-3"> <p> The <a href="./">web upload page</a> contains fields for adding metadata. Metadata is @@ -357,12 +357,12 @@ the web form. Here we add some extra information. </p> </div> -<div id="outline-container-org4c2e907" class="outline-3"> -<h3 id="org4c2e907"><span class="section-number-3">3.1</span> Obligatory fields</h3> +<div id="outline-container-orga044bef" class="outline-3"> +<h3 id="orga044bef"><span class="section-number-3">3.1</span> Obligatory fields</h3> <div class="outline-text-3" id="text-3-1"> </div> -<div id="outline-container-orgdddcb2e" class="outline-4"> -<h4 id="orgdddcb2e"><span class="section-number-4">3.1.1</span> Sample ID (sample<sub>id</sub>)</h4> +<div id="outline-container-org8e17492" class="outline-4"> +<h4 id="org8e17492"><span class="section-number-4">3.1.1</span> Sample ID (sample<sub>id</sub>)</h4> <div class="outline-text-4" id="text-3-1-1"> <p> This is a string field that defines a unique sample identifier by the @@ -380,8 +380,8 @@ Here we add the GenBank ID MT536190.1. </div> </div> -<div id="outline-container-orge9c2e76" class="outline-4"> -<h4 id="orge9c2e76"><span class="section-number-4">3.1.2</span> Collection date</h4> +<div id="outline-container-orgd9805db" class="outline-4"> +<h4 id="orgd9805db"><span class="section-number-4">3.1.2</span> Collection date</h4> <div class="outline-text-4" id="text-3-1-2"> <p> Estimated collection date. The GenBank page says April 6, 2020. @@ -389,8 +389,8 @@ Estimated collection date. The GenBank page says April 6, 2020. </div> </div> -<div id="outline-container-org62c55ce" class="outline-4"> -<h4 id="org62c55ce"><span class="section-number-4">3.1.3</span> Collection location</h4> +<div id="outline-container-org3bd4901" class="outline-4"> +<h4 id="org3bd4901"><span class="section-number-4">3.1.3</span> Collection location</h4> <div class="outline-text-4" id="text-3-1-3"> <p> A search on wikidata says Los Angeles is @@ -399,8 +399,8 @@ A search on wikidata says Los Angeles is </div> </div> -<div id="outline-container-org460b377" class="outline-4"> -<h4 id="org460b377"><span class="section-number-4">3.1.4</span> Sequencing technology</h4> +<div id="outline-container-org921de27" class="outline-4"> +<h4 id="org921de27"><span class="section-number-4">3.1.4</span> Sequencing technology</h4> <div class="outline-text-4" id="text-3-1-4"> <p> GenBank entry says Illumina, so we can fill that in @@ -408,8 +408,8 @@ GenBank entry says Illumina, so we can fill that in </div> </div> -<div id="outline-container-org77b1e14" class="outline-4"> -<h4 id="org77b1e14"><span class="section-number-4">3.1.5</span> Authors</h4> +<div id="outline-container-org39fa678" class="outline-4"> +<h4 id="org39fa678"><span class="section-number-4">3.1.5</span> Authors</h4> <div class="outline-text-4" id="text-3-1-5"> <p> GenBank entry says 'Lamers,S., Nolan,D.J., Rose,R., Cross,S., Moraga @@ -420,16 +420,16 @@ Freehan,A. and Garcia-Diaz,J.', so we can fill that in. </div> </div> -<div id="outline-container-org3cb346f" class="outline-3"> -<h3 id="org3cb346f"><span class="section-number-3">3.2</span> Optional fields</h3> +<div id="outline-container-org5315804" class="outline-3"> +<h3 id="org5315804"><span class="section-number-3">3.2</span> Optional fields</h3> <div class="outline-text-3" id="text-3-2"> <p> All other fields are optional. But let's see what we can add. </p> </div> -<div id="outline-container-orgb0cffbb" class="outline-4"> -<h4 id="orgb0cffbb"><span class="section-number-4">3.2.1</span> Host information</h4> +<div id="outline-container-orgf2b82d9" class="outline-4"> +<h4 id="orgf2b82d9"><span class="section-number-4">3.2.1</span> Host information</h4> <div class="outline-text-4" id="text-3-2-1"> <p> Sadly, not much is known about the host from GenBank. A little @@ -443,8 +443,8 @@ did to the person and what the person was like (say age group). </div> </div> -<div id="outline-container-orgd2a43a6" class="outline-4"> -<h4 id="orgd2a43a6"><span class="section-number-4">3.2.2</span> Collecting institution</h4> +<div id="outline-container-org8986ca7" class="outline-4"> +<h4 id="org8986ca7"><span class="section-number-4">3.2.2</span> Collecting institution</h4> <div class="outline-text-4" id="text-3-2-2"> <p> We can fill that in. @@ -452,8 +452,8 @@ We can fill that in. </div> </div> -<div id="outline-container-org8d5bcf7" class="outline-4"> -<h4 id="org8d5bcf7"><span class="section-number-4">3.2.3</span> Specimen source</h4> +<div id="outline-container-orge03eb0c" class="outline-4"> +<h4 id="orge03eb0c"><span class="section-number-4">3.2.3</span> Specimen source</h4> <div class="outline-text-4" id="text-3-2-3"> <p> We have that: nasopharyngeal swab @@ -461,8 +461,8 @@ We have that: nasopharyngeal swab </div> </div> -<div id="outline-container-org86b21b2" class="outline-4"> -<h4 id="org86b21b2"><span class="section-number-4">3.2.4</span> Source database accession</h4> +<div id="outline-container-org6815a6e" class="outline-4"> +<h4 id="org6815a6e"><span class="section-number-4">3.2.4</span> Source database accession</h4> <div class="outline-text-4" id="text-3-2-4"> <p> Genbank which is <a href="http://identifiers.org/insdc/MT536190.1#sequence">http://identifiers.org/insdc/MT536190.1#sequence</a>. @@ -471,8 +471,8 @@ Note we plug in our own identifier MT536190.1. </div> </div> -<div id="outline-container-org771ea66" class="outline-4"> -<h4 id="org771ea66"><span class="section-number-4">3.2.5</span> Strain name</h4> +<div id="outline-container-org51b37e8" class="outline-4"> +<h4 id="org51b37e8"><span class="section-number-4">3.2.5</span> Strain name</h4> <div class="outline-text-4" id="text-3-2-5"> <p> SARS-CoV-2/human/USA/LA-BIE-070/2020 @@ -482,8 +482,8 @@ SARS-CoV-2/human/USA/LA-BIE-070/2020 </div> </div> -<div id="outline-container-org7d281f5" class="outline-2"> -<h2 id="org7d281f5"><span class="section-number-2">4</span> Step 3: Submit to COVID-19 PubSeq</h2> +<div id="outline-container-org5778da6" class="outline-2"> +<h2 id="org5778da6"><span class="section-number-2">4</span> Step 3: Submit to COVID-19 PubSeq</h2> <div class="outline-text-2" id="text-4"> <p> Once you have the sequence and the metadata together, hit @@ -493,8 +493,8 @@ submitted and the workflows should kick in! </div> -<div id="outline-container-orgdf0f02d" class="outline-3"> -<h3 id="orgdf0f02d"><span class="section-number-3">4.1</span> Trouble shooting</h3> +<div id="outline-container-orge803d65" class="outline-3"> +<h3 id="orge803d65"><span class="section-number-3">4.1</span> Trouble shooting</h3> <div class="outline-text-3" id="text-4-1"> <p> We got an error saying: {"stem": "<a href="http://www.wikidata.org/entity/">http://www.wikidata.org/entity/</a>",… @@ -508,9 +508,8 @@ submit button. </div> </div> - -<div id="outline-container-org29f8a92" class="outline-2"> -<h2 id="org29f8a92"><span class="section-number-2">5</span> Step 4: Check output</h2> +<div id="outline-container-org540cfdf" class="outline-2"> +<h2 id="org540cfdf"><span class="section-number-2">5</span> Step 4: Check output</h2> <div class="outline-text-2" id="text-5"> <p> The current pipeline takes 5.5 hours to complete! Once it completes @@ -521,8 +520,8 @@ in. </div> </div> -<div id="outline-container-orgf493854" class="outline-2"> -<h2 id="orgf493854"><span class="section-number-2">6</span> Bulk sequence uploader</h2> +<div id="outline-container-org6c43ab3" class="outline-2"> +<h2 id="org6c43ab3"><span class="section-number-2">6</span> Bulk sequence uploader</h2> <div class="outline-text-2" id="text-6"> <p> Above steps require a manual upload of one sequence with metadata. @@ -544,8 +543,8 @@ host: host_age: <span style="color: #8bc34a;">20</span> host_age_unit: http://purl.obolibrary.org/obo/UO_<span style="color: #8bc34a;">0000036</span> host_health_status: http://purl.obolibrary.org/obo/NCIT_C<span style="color: #8bc34a;">25269</span> - host_treatment: Process in which the act is intended to modify or alter host status <span style="color: #e91e63;">(</span>Compounds<span style="color: #e91e63;">)</span> - host_vaccination: <span style="color: #e91e63;">[</span>vaccines<span style="color: #8bc34a;">1</span>,vaccine<span style="color: #8bc34a;">2</span><span style="color: #e91e63;">]</span> + host_treatment: Process in which the act is intended to modify or alter host status (Compounds) + host_vaccination: [vaccines<span style="color: #8bc34a;">1</span>,vaccine<span style="color: #8bc34a;">2</span>] ethnicity: http://purl.obolibrary.org/obo/HANCESTRO_<span style="color: #8bc34a;">0010</span> additional_host_information: Optional free text field for additional information @@ -553,11 +552,11 @@ sample: sample_id: Id of the sample as defined by the submitter collector_name: Name of the person that took the sample collecting_institution: Institute that was responsible of sampling - specimen_source: <span style="color: #e91e63;">[</span>http://purl.obolibrary.org/obo/NCIT_C<span style="color: #8bc34a;">155831</span>,http://purl.obolibrary.org/obo/NCIT_C<span style="color: #8bc34a;">155835</span>] + specimen_source: [http://purl.obolibrary.org/obo/NCIT_C<span style="color: #8bc34a;">155831</span>,http://purl.obolibrary.org/obo/NCIT_C<span style="color: #8bc34a;">155835</span>] collection_date: <span style="color: #9ccc65;">"2020-01-01"</span> collection_location: http://www.wikidata.org/entity/Q<span style="color: #8bc34a;">148</span> sample_storage_conditions: frozen specimen - source_database_accession: <span style="color: #2196F3;">[</span>http://identifiers.org/insdc/LC<span style="color: #8bc34a;">522350.1</span>#sequence] + source_database_accession: [http://identifiers.org/insdc/LC<span style="color: #8bc34a;">522350.1</span>#sequence] additional_collection_information: Optional free text field for additional information virus: @@ -565,28 +564,28 @@ virus: virus_strain: SARS-CoV-<span style="color: #8bc34a;">2</span>/human/CHN/HS_<span style="color: #8bc34a;">8</span>/<span style="color: #8bc34a;">2020</span> technology: - sample_sequencing_technology: <span style="color: #EF6C00;">[</span>http://www.ebi.ac.uk/efo/EFO_<span style="color: #8bc34a;">0009173</span>,http://www.ebi.ac.uk/efo/EFO_<span style="color: #8bc34a;">0009173</span>] + sample_sequencing_technology: [http://www.ebi.ac.uk/efo/EFO_<span style="color: #8bc34a;">0009173</span>,http://www.ebi.ac.uk/efo/EFO_<span style="color: #8bc34a;">0009173</span>] sequence_assembly_method: Protocol used for assembly - sequencing_coverage: <span style="color: #B388FF;">[</span><span style="color: #8bc34a;">70.0</span>, <span style="color: #8bc34a;">100.0</span><span style="color: #B388FF;">]</span> + sequencing_coverage: [<span style="color: #8bc34a;">70.0</span>, <span style="color: #8bc34a;">100.0</span>] additional_technology_information: Optional free text field for additional information submitter: - authors: <span style="color: #B388FF;">[</span>John Doe, Joe Boe, Jonny Oe<span style="color: #B388FF;">]</span> - submitter_name: <span style="color: #B388FF;">[</span>John Doe<span style="color: #B388FF;">]</span> + authors: [John Doe, Joe Boe, Jonny Oe] + submitter_name: [John Doe] submitter_address: John Doe's address originating_lab: John Doe kitchen lab_address: John Doe's address provider_sample_id: XXX<span style="color: #8bc34a;">1</span> submitter_sample_id: XXX<span style="color: #8bc34a;">2</span> publication: PMID<span style="color: #8bc34a;">00001113</span> - submitter_orcid: <span style="color: #B388FF;">[</span>https://orcid.org/<span style="color: #8bc34a;">0000</span>-<span style="color: #8bc34a;">0000</span>-<span style="color: #8bc34a;">0000</span>-<span style="color: #8bc34a;">0000</span>,https://orcid.org/<span style="color: #8bc34a;">0000</span>-<span style="color: #8bc34a;">0000</span>-<span style="color: #8bc34a;">0000</span>-<span style="color: #8bc34a;">0001</span>] + submitter_orcid: [https://orcid.org/<span style="color: #8bc34a;">0000</span>-<span style="color: #8bc34a;">0000</span>-<span style="color: #8bc34a;">0000</span>-<span style="color: #8bc34a;">0000</span>,https://orcid.org/<span style="color: #8bc34a;">0000</span>-<span style="color: #8bc34a;">0000</span>-<span style="color: #8bc34a;">0000</span>-<span style="color: #8bc34a;">0001</span>] additional_submitter_information: Optional free text field for additional information </pre> </div> </div> -<div id="outline-container-org37fadbc" class="outline-3"> -<h3 id="org37fadbc"><span class="section-number-3">6.1</span> Run the uploader (CLI)</h3> +<div id="outline-container-org99bb8b7" class="outline-3"> +<h3 id="org99bb8b7"><span class="section-number-3">6.1</span> Run the uploader (CLI)</h3> <div class="outline-text-3" id="text-6-1"> <p> Installing with pip you should be @@ -621,20 +620,35 @@ The web interface using this exact same script so it should just work </div> </div> -<div id="outline-container-org39adf09" class="outline-3"> -<h3 id="org39adf09"><span class="section-number-3">6.2</span> Example: uploading bulk GenBank sequences</h3> +<div id="outline-container-orga88593f" class="outline-3"> +<h3 id="orga88593f"><span class="section-number-3">6.2</span> Example: uploading bulk GenBank sequences</h3> <div class="outline-text-3" id="text-6-2"> <p> We also use above script to bulk upload GenBank sequences with a <a href="https://github.com/arvados/bh20-seq-resource/blob/master/scripts/download_genbank_data/from_genbank_to_fasta_and_yaml.py">FASTA and YAML</a> extractor specific for GenBank. This means that the steps we took above for uploading a GenBank sequence are already automated. </p> + +<p> +The steps are: from the +<code>bh20-seq-resource/scripts/download_genbank_data/</code> directory +</p> + +<div class="org-src-container"> +<pre class="src src-sh">python3 from_genbank_to_fasta_and_yaml.py +<span style="color: #ffcc80;">dir_fasta_and_yaml</span>=~/bh20-seq-resource/scripts/download_genbank_data/fasta_and_yaml +ls $<span style="color: #ffcc80;">dir_fasta_and_yaml</span>/*.yaml | <span style="color: #fff59d;">while </span><span style="color: #ff8A65;">read</span> path_code_yaml; <span style="color: #fff59d;">do</span> + <span style="color: #ffcc80;">path_code_fasta</span>=${<span style="color: #ffcc80;">path_code_yaml</span>%.*}.fasta + bh20-seq-uploader --skip-qc $<span style="color: #ffcc80;">path_code_yaml</span> $<span style="color: #ffcc80;">path_code_fasta</span> +<span style="color: #fff59d;">done</span> +</pre> +</div> </div> </div> </div> </div> <div id="postamble" class="status"> -<hr><small>Created by <a href="http://thebird.nl/">Pjotr Prins</a> (pjotr.public768 at thebird 'dot' nl) using Emacs org-mode and a healthy dose of Lisp!<br />Modified 2020-05-30 Sat 18:12</small>. +<hr><small>Created by <a href="http://thebird.nl/">Pjotr Prins</a> (pjotr.public768 at thebird 'dot' nl) using Emacs org-mode and a healthy dose of Lisp!<br />Modified 2020-08-24 Mon 04:31</small>. </div> </body> </html> |