diff options
Diffstat (limited to 'README.md')
-rw-r--r-- | README.md | 48 |
1 files changed, 48 insertions, 0 deletions
diff --git a/README.md b/README.md new file mode 100644 index 0000000..ec9afb1 --- /dev/null +++ b/README.md @@ -0,0 +1,48 @@ +# Sequence uploader + +This repository provides a sequence uploader for the + +# Run + +Run the uploader with a FASTA file and accompanying metadata: + + python3 bh20sequploader/main.py example/sequence.fasta example/metadata.json + +# Add a workflow + +get your SARS-CoV-2 sequences from GenBank in seqs.fa + +```sh +minimap2 -cx asm20 -X seqs.fa seqs.fa >seqs.paf +seqwish -s seqs.fa -p seqs.paf -g seqs.gfa +odgi build -g seqs.gfa -s -o seqs.odgi +odgi viz -i seqs.odgi -o seqs.png -x 4000 -y 500 -R -P 5 +``` + +from https://github.com/virtual-biohackathons/covid-19-bh20/wiki/Pangenome#pangenome-model-from-available-genomes + +# Installation + +This tool requires the arvados Python module which can be installed +using .deb or .rpm packages through +https://doc.arvados.org/v2.0/sdk/python/sdk-python.html. The actual +code lives [here](https://github.com/arvados/arvados/tree/master/sdk/python) and +suggests a local install using + + apt-get install libcurl4-openssl-dev libssl1.0-dev + pip3 install --user arvados-python-client + +Next update + + export PATH=$PATH:$HOME/.local/bin + +## Install with GNU Guix + +Set up a container: + + ~/opt/guix/bin/guix environment -C guix --ad-hoc python openssl python-pycurl nss-certs + pip3 install --user arvados-python-client + +Pip installed the following modules + + arvados-python-client-2.0.1 ciso8601-2.1.3 future-0.18.2 google-api-python-client-1.6.7 httplib2-0.17.1 oauth2client-4.1.3 pyasn1-0.4.8 pyasn1-modules-0.2.8 rsa-4.0 ruamel.yaml-0.15.77 six-1.14.0 uritemplate-3.0.1 ws4py-0.5.1 |