diff options
19 files changed, 441 insertions, 184 deletions
diff --git a/bh20seqanalyzer/main.py b/bh20seqanalyzer/main.py index 9a36cae..1746587 100644 --- a/bh20seqanalyzer/main.py +++ b/bh20seqanalyzer/main.py @@ -20,26 +20,36 @@ def validate_upload(api, collection, validated_project, fastq_project, fastq_workflow_uuid): col = arvados.collection.Collection(collection["uuid"]) + if collection.get("status") in ("validated", "rejected"): + return False + # validate the collection here. Check metadata, etc. - valid = True + logging.info("Validating upload '%s' (%s)" % (collection["name"], collection["uuid"])) - if "metadata.yaml" not in col: - logging.warn("Upload '%s' missing metadata.yaml", collection["name"]) - valid = False - else: - try: - metadata_content = ruamel.yaml.round_trip_load(col.open("metadata.yaml")) - metadata_content["id"] = "http://arvados.org/keep:%s/metadata.yaml" % collection["portable_data_hash"] - sample_id = metadata_content["sample"]["sample_id"] - add_lc_filename(metadata_content, metadata_content["id"]) - valid = qc_metadata(metadata_content) and valid - except Exception as e: - logging.warn(e) - valid = False - if not valid: - logging.warn("Failed metadata qc") - - if valid: + errors = [] + + dup = api.collections().list(filters=[["owner_uuid", "=", validated_project], + ["portable_data_hash", "=", col.portable_data_hash()]]).execute() + if dup["items"]: + # This exact collection has been uploaded before. + errors.append("Duplicate of %s" % ([d["uuid"] for d in dup["items"]])) + + if not errors: + if "metadata.yaml" not in col: + errors.append("Missing metadata.yaml", collection["name"]) + else: + try: + metadata_content = ruamel.yaml.round_trip_load(col.open("metadata.yaml")) + metadata_content["id"] = "http://arvados.org/keep:%s/metadata.yaml" % collection["portable_data_hash"] + sample_id = metadata_content["sample"]["sample_id"] + add_lc_filename(metadata_content, metadata_content["id"]) + valid = qc_metadata(metadata_content) + if not valid: + errors.append("Failed metadata qc") + except Exception as e: + errors.append(str(e)) + + if not errors: try: tgt = None paired = {"reads_1.fastq": "reads.fastq", "reads_1.fastq.gz": "reads.fastq.gz"} @@ -49,36 +59,32 @@ def validate_upload(api, collection, validated_project, with col.open(n, 'rb') as qf: tgt = qc_fasta(qf)[0] if tgt != n and tgt != paired.get(n): - logging.info("Expected %s but magic says it should be %s", n, tgt) - valid = False + errors.append("Expected %s but magic says it should be %s", n, tgt) elif tgt in ("reads.fastq", "reads.fastq.gz", "reads_1.fastq", "reads_1.fastq.gz"): start_fastq_to_fasta(api, collection, fastq_project, fastq_workflow_uuid, n, sample_id) return False if tgt is None: - valid = False - logging.warn("Upload '%s' does not contain sequence.fasta, reads.fastq or reads_1.fastq", collection["name"]) - except ValueError as v: - valid = False + errors.append("Upload '%s' does not contain sequence.fasta, reads.fastq or reads_1.fastq", collection["name"]) + except Exception as v: + errors.append(str(v)) - dup = api.collections().list(filters=[["owner_uuid", "=", validated_project], - ["portable_data_hash", "=", col.portable_data_hash()]]).execute() - if dup["items"]: - # This exact collection has been uploaded before. - valid = False - logging.warn("Upload '%s' is duplicate" % collection["name"]) - if valid: + if not errors: logging.info("Added '%s' to validated sequences" % collection["name"]) # Move it to the "validated" project to be included in the next analysis + collection["properties"]["status"] = "validated" api.collections().update(uuid=collection["uuid"], body={ "owner_uuid": validated_project, "name": "%s (%s)" % (collection["name"], time.asctime(time.gmtime()))}).execute() + return True else: - # It is invalid, delete it. - logging.warn("Suggest deleting '%s'" % collection["name"]) - #api.collections().delete(uuid=collection["uuid"]).execute() - - return valid + # It is invalid + logging.warn("'%s' (%s) has validation errors: %s" % ( + collection["name"], collection["uuid"], "\n".join(errors))) + collection["properties"]["status"] = "rejected" + collection["properties"]["errors"] = errors + api.collections().update(uuid=collection["uuid"], body={"properties": collection["properties"]}).execute() + return False def run_workflow(api, parent_project, workflow_uuid, name, inputobj): @@ -229,6 +235,50 @@ def upload_schema(api, workflow_def_project): return "keep:%s/schema.yml" % pdh +def print_status(api, uploader_project, fmt): + pending = arvados.util.list_all(api.collections().list, filters=[["owner_uuid", "=", uploader_project]]) + out = [] + status = {} + for p in pending: + prop = p["properties"] + out.append(prop) + if "status" not in prop: + prop["status"] = "pending" + prop["created_at"] = p["created_at"] + prop["uuid"] = p["uuid"] + status[prop["status"]] = status.get(prop["status"], 0) + 1 + if fmt == "html": + print( +""" +<html> +<body> +""") + print("<p>Total collections in upload project %s</p>" % len(out)) + print("<p>Status %s</p>" % status) + print( +""" +<table> +<tr><th>Collection</th> +<th>Sequence label</th> +<th>Status</th> +<th>Errors</th></tr> +""") + for r in out: + print("<tr valign='top'>") + print("<td><a href='https://workbench.lugli.arvadosapi.com/collections/%s'>%s</a></td>" % (r["uuid"], r["uuid"])) + print("<td>%s</td>" % r["sequence_label"]) + print("<td>%s</td>" % r["status"]) + print("<td><pre>%s</pre></td>" % "\n".join(r.get("errors", []))) + print("</tr>") + print( +""" +</table> +</body> +</html> +""") + else: + print(json.dumps(out, indent=2)) + def main(): parser = argparse.ArgumentParser(description='Analyze collections uploaded to a project') parser.add_argument('--uploader-project', type=str, default='lugli-j7d0g-n5clictpuvwk8aa', help='') @@ -244,7 +294,9 @@ def main(): parser.add_argument('--latest-result-collection', type=str, default='lugli-4zz18-z513nlpqm03hpca', help='') parser.add_argument('--kickoff', action="store_true") + parser.add_argument('--no-start-analysis', action="store_true") parser.add_argument('--once', action="store_true") + parser.add_argument('--print-status', type=str, default=None) args = parser.parse_args() api = arvados.api() @@ -263,20 +315,24 @@ def main(): args.exclude_list) return + if args.print_status: + print_status(api, args.uploader_project, args.print_status) + exit(0) + logging.info("Starting up, monitoring %s for uploads" % (args.uploader_project)) while True: move_fastq_to_fasta_results(api, args.fastq_project, args.uploader_project) - new_collections = api.collections().list(filters=[['owner_uuid', '=', args.uploader_project]]).execute() + new_collections = arvados.util.list_all(api.collections().list, filters=[["owner_uuid", "=", args.uploader_project]]) at_least_one_new_valid_seq = False - for c in new_collections["items"]: + for c in new_collections: at_least_one_new_valid_seq = validate_upload(api, c, args.validated_project, args.fastq_project, args.fastq_workflow_uuid) or at_least_one_new_valid_seq - if at_least_one_new_valid_seq: + if at_least_one_new_valid_seq and not args.no_start_analysis: start_pangenome_analysis(api, args.pangenome_analysis_project, args.pangenome_workflow_uuid, diff --git a/bh20sequploader/main.py b/bh20sequploader/main.py index c442af0..fd0278d 100644 --- a/bh20sequploader/main.py +++ b/bh20sequploader/main.py @@ -22,30 +22,32 @@ ARVADOS_API_HOST='lugli.arvadosapi.com' ARVADOS_API_TOKEN='2fbebpmbo3rw3x05ueu2i6nx70zhrsb1p22ycu3ry34m4x4462' UPLOAD_PROJECT='lugli-j7d0g-n5clictpuvwk8aa' -def qa_stuff(metadata, sequence_p1, sequence_p2): +def qc_stuff(metadata, sequence_p1, sequence_p2, do_qc=True): + failed = False try: - log.debug("Checking metadata") - if not qc_metadata(metadata.name): + log.debug("Checking metadata" if do_qc else "Skipping metadata check") + if do_qc and not qc_metadata(metadata.name): log.warning("Failed metadata qc") - exit(1) - except ValueError as e: + failed = True + except Exception as e: log.debug(e) - log.debug("Failed metadata qc") print(e) - exit(1) + failed = True target = [] try: - log.debug("Checking FASTA/FASTQ QC") - target.append(qc_fasta(sequence_p1)) + log.debug("FASTA/FASTQ QC" if do_qc else "Limited FASTA/FASTQ QC") + target.append(qc_fasta(sequence_p1, check_with_clustalw=do_qc)) if sequence_p2: target.append(qc_fasta(sequence_p2)) target[0] = ("reads_1."+target[0][0][6:], target[0][1]) target[1] = ("reads_2."+target[1][0][6:], target[0][1]) - except ValueError as e: + except Exception as e: log.debug(e) - log.debug("Failed FASTA qc") print(e) + failed = True + + if failed: exit(1) return target @@ -62,13 +64,14 @@ def main(): parser = argparse.ArgumentParser(description='Upload SARS-CoV-19 sequences for analysis') parser.add_argument('metadata', type=argparse.FileType('r'), help='sequence metadata json') parser.add_argument('sequence_p1', type=argparse.FileType('rb'), help='sequence FASTA/FASTQ') - parser.add_argument('sequence_p2', type=argparse.FileType('rb'), default=None, help='sequence FASTQ pair') + parser.add_argument('sequence_p2', type=argparse.FileType('rb'), default=None, nargs='?', help='sequence FASTQ pair') parser.add_argument("--validate", action="store_true", help="Dry run, validate only") + parser.add_argument("--skip-qc", action="store_true", help="Skip local qc check") args = parser.parse_args() api = arvados.api(host=ARVADOS_API_HOST, token=ARVADOS_API_TOKEN, insecure=True) - target = qa_stuff(args.metadata, args.sequence_p1, args.sequence_p2) + target = qc_stuff(args.metadata, args.sequence_p1, args.sequence_p2, not args.skip_qc) seqlabel = target[0][1] if args.validate: diff --git a/bh20sequploader/qc_fasta.py b/bh20sequploader/qc_fasta.py index e198430..37eb4e8 100644 --- a/bh20sequploader/qc_fasta.py +++ b/bh20sequploader/qc_fasta.py @@ -25,7 +25,7 @@ def read_fasta(sequence): raise ValueError("FASTA file contains multiple entries") return label, bases -def qc_fasta(arg_sequence): +def qc_fasta(arg_sequence, check_with_clustalw=True): log.debug("Starting qc_fasta") schema_resource = pkg_resources.resource_stream(__name__, "validation/formats") with tempfile.NamedTemporaryFile() as tmp: @@ -51,47 +51,48 @@ def qc_fasta(arg_sequence): if seq_type == "text/fasta": # ensure that contains only one entry submitlabel, submitseq = read_fasta(sequence) + sequence.seek(0) + sequence.detach() - with tempfile.NamedTemporaryFile() as tmp1: - refstring = pkg_resources.resource_string(__name__, "SARS-CoV-2-reference.fasta") - tmp1.write(refstring) - tmp1.write(submitlabel.encode("utf8")) - tmp1.write(("".join(submitseq)).encode("utf8")) - tmp1.flush() - subbp = 0 - refbp = 0 - similarity = 0 - try: - cmd = ["clustalw", "-infile="+tmp1.name, - "-quicktree", "-iteration=none", "-type=DNA"] - print("QC checking similarity to reference") - print(" ".join(cmd)) - result = subprocess.run(cmd, stdout=subprocess.PIPE) - res = result.stdout.decode("utf-8") - g1 = re.search(r"^Sequence 1: [^ ]+ +(\d+) bp$", res, flags=re.MULTILINE) - refbp = float(g1.group(1)) - g2 = re.search(r"^Sequence 2: [^ ]+ +(\d+) bp$", res, flags=re.MULTILINE) - subbp = float(g2.group(1)) - g3 = re.search(r"^Sequences \(1:2\) Aligned\. Score: (\d+(\.\d+)?)$", res, flags=re.MULTILINE) - similarity = float(g3.group(1)) + if not check_with_clustalw: + return ("sequence.fasta"+gz, seqlabel) - print(g1.group(0)) - print(g2.group(0)) - print(g3.group(0)) - except Exception as e: - logging.warn("Error trying to QC against reference sequence using 'clustalw': %s", e) + with tempfile.NamedTemporaryFile() as tmp1: + with tempfile.NamedTemporaryFile() as tmp2: + refstring = pkg_resources.resource_string(__name__, "SARS-CoV-2-reference.fasta") + tmp1.write(refstring) + tmp1.flush() + tmp2.write(submitlabel.encode("utf8")) + tmp2.write(("".join(submitseq)).encode("utf8")) + tmp2.flush() + subbp = 0 + refbp = 0 + similarity = 0 + try: + cmd = ["minimap2", "-c", tmp1.name, tmp2.name] + logging.info("QC checking similarity to reference") + logging.info(" ".join(cmd)) + result = subprocess.run(cmd, stdout=subprocess.PIPE) + result.check_returncode() + res = result.stdout.decode("utf-8") + mm = res.split("\t") + if len(mm) >= 10: + # divide Number of matching bases in the mapping / Target sequence length + similarity = (float(mm[9]) / float(mm[6])) * 100.0 + else: + similarity = 0 + except Exception as e: + logging.warn("QC against reference sequence using 'minimap2': %s", e, exc_info=e) - if refbp and (subbp/refbp) < .7: - raise ValueError("QC fail: submit sequence length is shorter than 70% reference") - if refbp and (subbp/refbp) > 1.3: - raise ValueError("QC fail: submit sequence length is greater than 130% reference") - if similarity and similarity < 70.0: - raise ValueError("QC fail: submit similarity is less than 70%") - if refbp == 0 or similarity == 0: - raise ValueError("QC fail") + if similarity and similarity < 70.0: + raise ValueError("QC fail: alignment to reference was less than 70%% (was %2.2f%%)" % (similarity)) + if similarity == 0: + raise ValueError("QC fail") return ("sequence.fasta"+gz, seqlabel) elif seq_type == "text/fastq": + sequence.seek(0) + sequence.detach() return ("reads.fastq"+gz, seqlabel) else: raise ValueError("Sequence file does not look like a DNA FASTA or FASTQ") diff --git a/bh20sequploader/qc_metadata.py b/bh20sequploader/qc_metadata.py index 9122ace..2b57991 100644 --- a/bh20sequploader/qc_metadata.py +++ b/bh20sequploader/qc_metadata.py @@ -21,20 +21,13 @@ def qc_metadata(metadatafile): shex = pkg_resources.resource_stream(__name__, "bh20seq-shex.rdf").read().decode("utf-8") if not isinstance(avsc_names, schema_salad.avro.schema.Names): - print(avsc_names) - return False + raise Exception(avsc_names) - try: - doc, metadata = schema_salad.schema.load_and_validate(document_loader, avsc_names, metadatafile, True) - g = schema_salad.jsonld_context.makerdf("workflow", doc, document_loader.ctx) - rslt, reason = evaluate(g, shex, doc["id"], "https://raw.githubusercontent.com/arvados/bh20-seq-resource/master/bh20sequploader/bh20seq-shex.rdf#submissionShape") + doc, metadata = schema_salad.schema.load_and_validate(document_loader, avsc_names, metadatafile, True) + g = schema_salad.jsonld_context.makerdf("workflow", doc, document_loader.ctx) + rslt, reason = evaluate(g, shex, doc["id"], "https://raw.githubusercontent.com/arvados/bh20-seq-resource/master/bh20sequploader/bh20seq-shex.rdf#submissionShape") - if not rslt: - log.debug(reason) - print(reason) + if not rslt: + raise Exception(reason) - return rslt - except Exception as e: - traceback.print_exc() - log.warn(e) - return False + return True diff --git a/scripts/create_sra_metadata/SraExperimentPackage.2020.07.05.xml.gz b/scripts/create_sra_metadata/SraExperimentPackage.2020.07.05.xml.gz Binary files differnew file mode 100644 index 0000000..88acb18 --- /dev/null +++ b/scripts/create_sra_metadata/SraExperimentPackage.2020.07.05.xml.gz diff --git a/scripts/download_sra_data/download_sra_data.py b/scripts/create_sra_metadata/create_sra_metadata.py index 9145a43..ef0d119 100644 --- a/scripts/download_sra_data/download_sra_data.py +++ b/scripts/create_sra_metadata/create_sra_metadata.py @@ -8,9 +8,11 @@ import gzip dir_yaml = 'yaml' -date = '2020.06.08' +date = '2020.07.05' -# Query on SRA: 'txid2697049[Organism]' (https://www.ncbi.nlm.nih.gov/sra/?term=txid2697049%5BOrganism%5D) -> Send to -> File -> Full XML -> Create File +# Query on SRA: 'txid2697049[Organism]' (https://www.ncbi.nlm.nih.gov/sra/?term=txid2697049%5BOrganism%5D) +# Query on SRA: 'txid2697049[Organism:noexp] NOT 0[Mbases ' (https://www.ncbi.nlm.nih.gov/sra/?term=txid2697049%5BOrganism:noexp%5D%20NOT%200[Mbases) +# -> Send to -> File -> Full XML -> Create File path_sra_metadata_xml = 'SraExperimentPackage.{}.xml.gz'.format(date) dir_dict_ontology_standardization = '../dict_ontology_standardization/' @@ -70,14 +72,14 @@ for i, EXPERIMENT_PACKAGE in enumerate(EXPERIMENT_PACKAGE_SET): accession = RUN.attrib['accession'] run_accession_set.add(accession) #print(accession) - + info_for_yaml_dict['sample']['sample_id'] = accession - SRAFiles = RUN.find('SRAFiles') - if SRAFiles is not None: - url = SRAFiles.find('SRAFile').attrib['url'] - if 'sra-download.ncbi.nlm.nih.gov' in url: - run_accession_to_downloadble_file_url_dict[accession] = url + #SRAFiles = RUN.find('SRAFiles') + #if SRAFiles is not None: + # url = SRAFiles.find('SRAFile').attrib['url'] + # if 'sra-download.ncbi.nlm.nih.gov' in url: + # run_accession_to_downloadble_file_url_dict[accession] = url SAMPLE = EXPERIMENT_PACKAGE.find('SAMPLE') @@ -90,6 +92,9 @@ for i, EXPERIMENT_PACKAGE in enumerate(EXPERIMENT_PACKAGE_SET): VALUE_text = VALUE.text if TAG_text in ['host', 'host scientific name']: + if VALUE_text.lower() in ['homo sapien', 'homosapiens']: + VALUE_text = 'Homo sapiens' + if VALUE_text in term_to_uri_dict: info_for_yaml_dict['host']['host_species'] = term_to_uri_dict[VALUE_text] else: @@ -100,11 +105,19 @@ for i, EXPERIMENT_PACKAGE in enumerate(EXPERIMENT_PACKAGE_SET): elif VALUE_text.strip("'") not in ['missing', 'not collected', 'not provided']: missing_value_list.append('\t'.join([accession, 'host_health_status', VALUE_text])) elif TAG_text in ['strain', 'isolate']: - if VALUE_text.lower() not in ['not applicable', 'missing', 'na', 'unknown']: - if 'virus_strain' not in info_for_yaml_dict: - info_for_yaml_dict['virus']['virus_strain'] = VALUE_text + if VALUE_text.lower() not in ['not applicable', 'missing', 'na', 'unknown', 'not provided']: + value_to_insert = VALUE_text + + if value_to_insert.lower() in ['homo sapien', 'homosapiens']: + value_to_insert = 'Homo sapiens' + + if value_to_insert in term_to_uri_dict: + value_to_insert = term_to_uri_dict[value_to_insert] + + if 'virus_strain' not in info_for_yaml_dict: + info_for_yaml_dict['virus']['virus_strain'] = value_to_insert else: - info_for_yaml_dict['virus']['virus_strain'] += '; ' + VALUE_text + info_for_yaml_dict['virus']['virus_strain'] += '; ' + value_to_insert elif TAG_text in ['isolation_source', 'isolation source host-associated']: if VALUE_text in term_to_uri_dict: info_for_yaml_dict['sample']['specimen_source'] = [term_to_uri_dict[VALUE_text]] @@ -179,17 +192,18 @@ for i, EXPERIMENT_PACKAGE in enumerate(EXPERIMENT_PACKAGE_SET): EXPERIMENT = EXPERIMENT_PACKAGE.find('EXPERIMENT') INSTRUMENT_MODEL = [x.text for x in EXPERIMENT.find('PLATFORM').iter('INSTRUMENT_MODEL')][0] + if INSTRUMENT_MODEL.lower() != 'unspecified': if INSTRUMENT_MODEL in term_to_uri_dict: info_for_yaml_dict['technology']['sample_sequencing_technology'] = [term_to_uri_dict[INSTRUMENT_MODEL]] else: missing_value_list.append('\t'.join([accession, 'sample_sequencing_technology', INSTRUMENT_MODEL])) - + #else: + # print(accession, 'Missing INSTRUMENT_MODEL', info_for_yaml_dict) LIBRARY_DESCRIPTOR = EXPERIMENT.find('DESIGN').find('LIBRARY_DESCRIPTOR') if LIBRARY_DESCRIPTOR.text not in ['OTHER']: info_for_yaml_dict['technology']['additional_technology_information'] = 'LIBRARY_STRATEGY: {};'.format(LIBRARY_DESCRIPTOR.find('LIBRARY_STRATEGY').text) - - + SUBMISSION = EXPERIMENT_PACKAGE.find('SUBMISSION') info_for_yaml_dict['submitter']['submitter_sample_id'] = SUBMISSION.attrib['accession'] @@ -197,7 +211,7 @@ for i, EXPERIMENT_PACKAGE in enumerate(EXPERIMENT_PACKAGE_SET): info_for_yaml_dict['submitter']['originating_lab'] = SUBMISSION.attrib['lab_name'] STUDY = EXPERIMENT_PACKAGE.find('STUDY') - info_for_yaml_dict['submitter']['publication'] = SUBMISSION.attrib['lab_name'] + info_for_yaml_dict['submitter']['publication'] = STUDY.attrib['alias'] Organization = EXPERIMENT_PACKAGE.find('Organization') @@ -222,6 +236,10 @@ for i, EXPERIMENT_PACKAGE in enumerate(EXPERIMENT_PACKAGE_SET): info_for_yaml_dict['sample']['collection_date'] = '1970-01-01' info_for_yaml_dict['sample']['additional_collection_information'] = "The real 'collection_date' is missing" + if 'sample_sequencing_technology' not in info_for_yaml_dict['technology']: + print(accession, ' - technology not found') + continue + with open(os.path.join(dir_yaml, '{}.yaml'.format(accession)), 'w') as fw: json.dump(info_for_yaml_dict, fw, indent=2) @@ -229,4 +247,4 @@ if len(missing_value_list) > 0: path_missing_terms_tsv = 'missing_terms.tsv' print('Written missing terms in {}'.format(path_missing_terms_tsv)) with open(path_missing_terms_tsv, 'w') as fw: - fw.write('\n'.join(missing_value_list))
\ No newline at end of file + fw.write('\n'.join(missing_value_list)) diff --git a/scripts/dict_ontology_standardization/ncbi_countries.csv b/scripts/dict_ontology_standardization/ncbi_countries.csv index 204f7f2..4bea3ec 100644 --- a/scripts/dict_ontology_standardization/ncbi_countries.csv +++ b/scripts/dict_ontology_standardization/ncbi_countries.csv @@ -284,6 +284,7 @@ USA:CA,http://www.wikidata.org/entity/Q99 USA: California,http://www.wikidata.org/entity/Q99 USA:California,http://www.wikidata.org/entity/Q99 "USA: CA, San Diego County",http://www.wikidata.org/entity/Q108143 +"USA: California, Monterey County",http://www.wikidata.org/entity/Q108072 USA: CO,http://www.wikidata.org/entity/Q1261 USA: CT,http://www.wikidata.org/entity/Q779 USA: Connecticut,http://www.wikidata.org/entity/Q779 @@ -301,8 +302,9 @@ USA: IN,http://www.wikidata.org/entity/Q1415 USA: KS,http://www.wikidata.org/entity/Q1558 USA: KY,http://www.wikidata.org/entity/Q1603 USA: LA,http://www.wikidata.org/entity/Q1588 -USA:Los Angeles,http://www.wikidata.org/entity/Q65 -"USA: New Orleans, LA",http://www.wikidata.org/entity/Q34404 +"USA: SLIDELL, LA, LA",https://www.wikidata.org/wiki/Q988156 +"USA: Slidell, LA, LA",https://www.wikidata.org/wiki/Q988156 +"USA: New Orleans, LA",https://www.wikidata.org/wiki/Q34404 USA: MA,http://www.wikidata.org/entity/Q771 USA: Massachusetts,http://www.wikidata.org/entity/Q771 USA: MD,http://www.wikidata.org/entity/Q1391 @@ -336,6 +338,33 @@ USA: SC,http://www.wikidata.org/entity/Q1456 USA: South Carolina,http://www.wikidata.org/entity/Q1456 USA: SD,http://www.wikidata.org/entity/Q1211 "USA: Snohomish County, WA",http://www.wikidata.org/entity/Q110403 +"USA: Washington,Snohomish County",http://www.wikidata.org/entity/Q110403 +"USA: Washington, Snohomish County",http://www.wikidata.org/entity/Q110403 +"USA: Washington,Skagit County",http://www.wikidata.org/entity/Q113892 +"USA: Washington, Skagit County",http://www.wikidata.org/entity/Q113892 +"USA: Washington,Pierce County",http://www.wikidata.org/entity/Q156459 +"USA: Washington, Pierce County",http://www.wikidata.org/entity/Q156459 +"USA: Washington,Mason County",http://www.wikidata.org/entity/Q111904 +"USA: Washington, Mason County",http://www.wikidata.org/entity/Q111904 +"USA: Washington,Kittitas County",http://www.wikidata.org/entity/Q111540 +"USA: Washington,King County",http://www.wikidata.org/entity/Q108861 +"USA: Washington, King County",http://www.wikidata.org/entity/Q108861 +"USA: King County,WA",http://www.wikidata.org/entity/Q108861 +"USA: Washington,Jefferson County",http://www.wikidata.org/entity/Q384737 +"USA: Washington,Grant County",http://www.wikidata.org/entity/Q281681 +"USA: Washington, Grant County",http://www.wikidata.org/entity/Q281681 +"USA: Washington,Franklin County",http://www.wikidata.org/entity/Q118716 +"USA: Washington, Franklin County",http://www.wikidata.org/entity/Q118716 +"USA: Washington,Clark County",http://www.wikidata.org/entity/Q156287 +"USA: Washington,Benton County",http://www.wikidata.org/entity/Q156216 +"USA: Washington, Benton County",http://www.wikidata.org/entity/Q156216 +"USA: Washington,Asotin County",http://www.wikidata.org/entity/Q156295 +"USA: Washington, Asotin County",http://www.wikidata.org/entity/Q156295 +"USA: Washington,Adams County",http://www.wikidata.org/entity/Q156273 +"USA: Washington, Adams County",http://www.wikidata.org/entity/Q156273 +"USA: Washington, Spokane County",http://www.wikidata.org/entity/Q485276 +"USA: Washington, Douglas County",http://www.wikidata.org/entity/Q156220 +"USA: Washington, Cowlitz County",http://www.wikidata.org/entity/Q156276 USA: TN,http://www.wikidata.org/entity/Q1509 USA: TX,http://www.wikidata.org/entity/Q1439 USA: UT,http://www.wikidata.org/entity/Q829 @@ -347,6 +376,10 @@ USA: WA,http://www.wikidata.org/entity/Q1223 USA: Washington,http://www.wikidata.org/entity/Q1223 USA: WI,http://www.wikidata.org/entity/Q1537 USA: Wisconsin,http://www.wikidata.org/entity/Q1537 +"USA: Washington,Yakima County",http://www.wikidata.org/entity/Q156629 +"USA: Washington, Yakima County",http://www.wikidata.org/entity/Q156629 +"USA: Washington,Whatcom County",http://www.wikidata.org/entity/Q156623 +"USA: Dane County, Wisconsin",http://www.wikidata.org/entity/Q502200 USA: WV,http://www.wikidata.org/entity/Q1371 USA: WY,http://www.wikidata.org/entity/Q1214 Uzbekistan,http://www.wikidata.org/entity/Q265 diff --git a/scripts/dict_ontology_standardization/ncbi_host_species.csv b/scripts/dict_ontology_standardization/ncbi_host_species.csv index bc6ac04..40572a3 100644 --- a/scripts/dict_ontology_standardization/ncbi_host_species.csv +++ b/scripts/dict_ontology_standardization/ncbi_host_species.csv @@ -5,5 +5,6 @@ sapiens,http://purl.obolibrary.org/obo/NCBITaxon_9606 Mustela lutreola,http://purl.obolibrary.org/obo/NCBITaxon_9666 Manis javanica,http://purl.obolibrary.org/obo/NCBITaxon_9974 Felis catus,http://purl.obolibrary.org/obo/NCBITaxon_9685 +Felis catus; Domestic Shorthair,http://purl.obolibrary.org/obo/NCBITaxon_9685 Panthera tigris jacksoni,http://purl.obolibrary.org/obo/NCBITaxon_419130 Canis lupus familiaris,http://purl.obolibrary.org/obo/NCBITaxon_9615 diff --git a/scripts/dict_ontology_standardization/ncbi_sequencing_technology.csv b/scripts/dict_ontology_standardization/ncbi_sequencing_technology.csv index 964cbf3..59c4800 100644 --- a/scripts/dict_ontology_standardization/ncbi_sequencing_technology.csv +++ b/scripts/dict_ontology_standardization/ncbi_sequencing_technology.csv @@ -13,12 +13,14 @@ Illumina NextSeq 550,http://www.ebi.ac.uk/efo/EFO_0008566 NextSeq550,http://www.ebi.ac.uk/efo/EFO_0008566 NextSeq 550,http://www.ebi.ac.uk/efo/EFO_0008566 Illumina MiniSeq,http://www.ebi.ac.uk/efo/EFO_0008636 +Illumina NovaSeq,http://www.ebi.ac.uk/efo/EFO_0008637 Illumina NovaSeq 6000,http://www.ebi.ac.uk/efo/EFO_0008637 Nanopore MinION,http://www.ebi.ac.uk/efo/EFO_0008632 Oxford Nanopore MinION,http://www.ebi.ac.uk/efo/EFO_0008632 ONT (Oxford Nanopore Technologies),http://purl.obolibrary.org/obo/NCIT_C146818 Oxford Nanopore Technology,http://purl.obolibrary.org/obo/NCIT_C146818 Oxford Nanopore technologies MinION,http://www.ebi.ac.uk/efo/EFO_0008632 +Oxford Nanopore Sequencing,http://purl.obolibrary.org/obo/NCIT_C146818 MinION Oxford Nanopore,http://www.ebi.ac.uk/efo/EFO_0008632 MinION,http://www.ebi.ac.uk/efo/EFO_0008632 Nanopore,http://purl.obolibrary.org/obo/NCIT_C146818 @@ -33,3 +35,24 @@ ThermoFisher S5Plus,http://purl.obolibrary.org/obo/NCIT_C125894 Sanger dideoxy sequencing,http://purl.obolibrary.org/obo/NCIT_C19641 MGISEQ 2000,http://virtual-bh/MGISEQ2000 MGISEQ2000,http://virtual-bh/MGISEQ2000 +Illumina HiSeq X,http://www.ebi.ac.uk/efo/EFO_0008567 +ONT GridION X5,http://www.ebi.ac.uk/efo/EFO_0008633 +ONT PremethION,http://www.ebi.ac.uk/efo/EFO_0008634 +PacBio RS II,http://www.ebi.ac.uk/efo/EFO_0008631 +PacBio Sequel System,http://www.ebi.ac.uk/efo/EFO_0008630 +Illumina Genome Analyzer,http://www.ebi.ac.uk/efo/EFO_0004200 +Illumina Genome Analyzer II,http://www.ebi.ac.uk/efo/EFO_0004201 +Illumina Genome Analyzer IIx,http://www.ebi.ac.uk/efo/EFO_0004202 +454 GS 20 sequencer,http://www.ebi.ac.uk/efo/EFO_0004206 +454 GS FLX Titanium sequencer,http://www.ebi.ac.uk/efo/EFO_0004433 +454 GS FLX sequencer,http://www.ebi.ac.uk/efo/EFO_0004432 +454 GS Junior sequencer,http://www.ebi.ac.uk/efo/EFO_0004434 +454 GS sequencer,http://www.ebi.ac.uk/efo/EFO_0004431 +AB SOLiD 4 System,http://www.ebi.ac.uk/efo/EFO_0004438 +AB SOLiD 4hq System,http://www.ebi.ac.uk/efo/EFO_0004441 +AB SOLiD 5500,http://www.ebi.ac.uk/efo/EFO_0004440 +AB SOLiD 5500xl,http://www.ebi.ac.uk/efo/EFO_0004436 +AB SOLiD PI System,http://www.ebi.ac.uk/efo/EFO_0004437 +AB SOLiD System,http://www.ebi.ac.uk/efo/EFO_0004435 +AB SOLiD System 2.0,http://www.ebi.ac.uk/efo/EFO_0004442 +AB SOLiD System 3.0,http://www.ebi.ac.uk/efo/EFO_0004439 diff --git a/scripts/docker/Dockerfile b/scripts/docker/Dockerfile index 9fb33d5..02829d4 100644 --- a/scripts/docker/Dockerfile +++ b/scripts/docker/Dockerfile @@ -3,8 +3,8 @@ FROM debian:10 RUN apt-get update && \ apt-get -yq --no-install-recommends -o Acquire::Retries=6 install \ python3 python3-pip python3-setuptools python3-dev python-pycurl \ - clustalw python3-biopython libcurl4-openssl-dev build-essential \ + minimap2 python3-biopython libcurl4-openssl-dev build-essential \ libssl-dev libmagic-dev python3-magic && \ apt-get clean -RUN pip3 install bh20-seq-uploader +RUN pip3 install bh20-seq-uploader py-dateutil diff --git a/scripts/download_genbank_data/from_genbank_to_fasta_and_yaml.py b/scripts/download_genbank_data/from_genbank_to_fasta_and_yaml.py index d76f56b..cb94787 100755 --- a/scripts/download_genbank_data/from_genbank_to_fasta_and_yaml.py +++ b/scripts/download_genbank_data/from_genbank_to_fasta_and_yaml.py @@ -4,6 +4,8 @@ import argparse parser = argparse.ArgumentParser() parser.add_argument('--skip-request', action='store_true', help='skip metadata and sequence request', required=False) parser.add_argument('--only-missing-id', action='store_true', help='download only missing id', required=False) +parser.add_argument('--dict-ontology', type=str, help='where is the ontology', + default='../dict_ontology_standardization/',required=False) args = parser.parse_args() from Bio import Entrez @@ -22,7 +24,7 @@ num_ids_for_request = 100 dir_metadata = 'metadata_from_nuccore' dir_fasta_and_yaml = 'fasta_and_yaml' -dir_dict_ontology_standardization = '../dict_ontology_standardization/' +dir_dict_ontology_standardization = args.dict_ontology today_date = date.today().strftime("%Y.%m.%d") path_ncbi_virus_accession = 'sequences.{}.acc'.format(today_date) @@ -126,7 +128,7 @@ for path_dict_xxx_csv in [os.path.join(dir_dict_ontology_standardization, name_x if term in term_to_uri_dict: print('Warning: in the dictionaries there are more entries for the same term ({}).'.format(term)) continue - + term_to_uri_dict[term] = uri if not os.path.exists(dir_fasta_and_yaml): @@ -171,7 +173,9 @@ for path_metadata_xxx_xml in [os.path.join(dir_metadata, name_metadata_xxx_xml) # submitter info GBSeq_references = GBSeq.find('GBSeq_references') if GBSeq_references is not None: - info_for_yaml_dict['submitter']['authors'] = ["{}".format(x.text) for x in GBSeq_references.iter('GBAuthor')] + author_list = ["{}".format(x.text) for x in GBSeq_references.iter('GBAuthor')] + if len(author_list) > 0: + info_for_yaml_dict['submitter']['authors'] = author_list GBReference = GBSeq_references.find('GBReference') if GBReference is not None: @@ -220,7 +224,8 @@ for path_metadata_xxx_xml in [os.path.join(dir_metadata, name_metadata_xxx_xml) new_seq_tec_list.append(seq_tec) - info_for_yaml_dict['technology']['sample_sequencing_technology'] = [x for x in new_seq_tec_list] + if len(new_seq_tec_list) > 0: + info_for_yaml_dict['technology']['sample_sequencing_technology'] = [x for x in new_seq_tec_list] else: info_for_yaml_dict['technology'][field_in_yaml] = tech_info_to_parse @@ -238,58 +243,62 @@ for path_metadata_xxx_xml in [os.path.join(dir_metadata, name_metadata_xxx_xml) GBQualifier_name_text = GBQualifier.find('GBQualifier_name').text if GBQualifier_name_text == 'host': - GBQualifier_value_text_list = GBQualifier_value_text.split('; ') - - if GBQualifier_value_text_list[0] in term_to_uri_dict: - info_for_yaml_dict['host']['host_species'] = term_to_uri_dict[GBQualifier_value_text_list[0]] - elif GBQualifier_value_text_list[0] and ('MT215193' in accession_version or 'MT270814' in accession_version): - # Information checked manually from NCBI Virus - info_for_yaml_dict['host']['host_species'] = term_to_uri_dict['Canis lupus familiaris'] + if GBQualifier_value_text in term_to_uri_dict: + # Cases like 'Felis catus; Domestic Shorthair' + info_for_yaml_dict['host']['host_species'] = term_to_uri_dict[GBQualifier_value_text] else: - missing_value_list.append('\t'.join([accession_version, 'host_species', GBQualifier_value_text_list[0]])) - - # Possible cases: - # - Homo sapiens --> ['Homo sapiens'] - # - Homo sapiens; female --> ['Homo sapiens', 'female'] - # - Homo sapiens; female 63 --> ['Homo sapiens', 'female 63'] - # - Homo sapiens; female; age 40 --> ['Homo sapiens', 'female', 'age 40'] - # - Homo sapiens; gender: F; age: 61 --> ['Homo sapiens', 'gender: F', 'age: 61'] - # - Homo sapiens; gender: M; age: 68 --> ['Homo sapiens', 'gender: M', 'age: 68'] - # - Homo sapiens; hospitalized patient --> ['Homo sapiens', 'hospitalized patient'] - # - Homo sapiens; male --> ['Homo sapiens', 'male'] - # - Homo sapiens; male; 63 --> ['Homo sapiens', 'male', '63'] - # - Homo sapiens; male; age 29 --> ['Homo sapiens', 'male', 'age 29'] - # - Homo sapiens; symptomatic --> ['Homo sapiens', 'symptomatic'] - if len(GBQualifier_value_text_list) > 1: - host_sex = '' - if 'female' in GBQualifier_value_text_list[1]: - host_sex = 'female' - elif 'male' in GBQualifier_value_text_list[1]: - host_sex = 'male' - elif 'gender' in GBQualifier_value_text_list[1]: - host_sex_one_lecter = GBQualifier_value_text_list[1].split(':')[-1].strip() - if host_sex_one_lecter in ['F', 'M']: - host_sex = 'female' if host_sex_one_lecter == 'F' else 'male' - - if host_sex in ['male', 'female']: - info_for_yaml_dict['host']['host_sex'] = "http://purl.obolibrary.org/obo/PATO_0000384" if host_sex == 'male' else "http://purl.obolibrary.org/obo/PATO_0000383" - elif GBQualifier_value_text_list[1] in term_to_uri_dict: - info_for_yaml_dict['host']['host_health_status'] = term_to_uri_dict[GBQualifier_value_text_list[1]] + GBQualifier_value_text_list = GBQualifier_value_text.split('; ') + + if GBQualifier_value_text_list[0] in term_to_uri_dict: + info_for_yaml_dict['host']['host_species'] = term_to_uri_dict[GBQualifier_value_text_list[0]] + elif GBQualifier_value_text_list[0] and ('MT215193' in accession_version or 'MT270814' in accession_version): + # Information checked manually from NCBI Virus + info_for_yaml_dict['host']['host_species'] = term_to_uri_dict['Canis lupus familiaris'] else: - missing_value_list.append('\t'.join([accession_version, 'host_sex or host_health_status', GBQualifier_value_text_list[1]])) - - # Host age - host_age = -1 - if len(GBQualifier_value_text_list[1].split(' ')) > 1 and is_integer(GBQualifier_value_text_list[1].split(' ')[-1]): - host_age = int(GBQualifier_value_text_list[1].split(' ')[-1]) - elif len(GBQualifier_value_text_list) > 2 and is_integer(GBQualifier_value_text_list[2].split(' ')[-1]): - host_age = int(GBQualifier_value_text_list[2].split(' ')[-1]) - - if host_age > -1: - info_for_yaml_dict['host']['host_age'] = host_age - info_for_yaml_dict['host']['host_age_unit'] = 'http://purl.obolibrary.org/obo/UO_0000036' - elif len(GBQualifier_value_text_list) > 2: - missing_value_list.append('\t'.join([accession_version, 'host_age', GBQualifier_value_text_list[2]])) + missing_value_list.append('\t'.join([accession_version, 'host_species', GBQualifier_value_text_list[0]])) + + # Possible cases: + # - Homo sapiens --> ['Homo sapiens'] + # - Homo sapiens; female --> ['Homo sapiens', 'female'] + # - Homo sapiens; female 63 --> ['Homo sapiens', 'female 63'] + # - Homo sapiens; female; age 40 --> ['Homo sapiens', 'female', 'age 40'] + # - Homo sapiens; gender: F; age: 61 --> ['Homo sapiens', 'gender: F', 'age: 61'] + # - Homo sapiens; gender: M; age: 68 --> ['Homo sapiens', 'gender: M', 'age: 68'] + # - Homo sapiens; hospitalized patient --> ['Homo sapiens', 'hospitalized patient'] + # - Homo sapiens; male --> ['Homo sapiens', 'male'] + # - Homo sapiens; male; 63 --> ['Homo sapiens', 'male', '63'] + # - Homo sapiens; male; age 29 --> ['Homo sapiens', 'male', 'age 29'] + # - Homo sapiens; symptomatic --> ['Homo sapiens', 'symptomatic'] + if len(GBQualifier_value_text_list) > 1: + host_sex = '' + if 'female' in GBQualifier_value_text_list[1]: + host_sex = 'female' + elif 'male' in GBQualifier_value_text_list[1]: + host_sex = 'male' + elif 'gender' in GBQualifier_value_text_list[1]: + host_sex_one_lecter = GBQualifier_value_text_list[1].split(':')[-1].strip() + if host_sex_one_lecter in ['F', 'M']: + host_sex = 'female' if host_sex_one_lecter == 'F' else 'male' + + if host_sex in ['male', 'female']: + info_for_yaml_dict['host']['host_sex'] = "http://purl.obolibrary.org/obo/PATO_0000384" if host_sex == 'male' else "http://purl.obolibrary.org/obo/PATO_0000383" + elif GBQualifier_value_text_list[1] in term_to_uri_dict: + info_for_yaml_dict['host']['host_health_status'] = term_to_uri_dict[GBQualifier_value_text_list[1]] + else: + missing_value_list.append('\t'.join([accession_version, 'host_sex or host_health_status', GBQualifier_value_text_list[1]])) + + # Host age + host_age = -1 + if len(GBQualifier_value_text_list[1].split(' ')) > 1 and is_integer(GBQualifier_value_text_list[1].split(' ')[-1]): + host_age = int(GBQualifier_value_text_list[1].split(' ')[-1]) + elif len(GBQualifier_value_text_list) > 2 and is_integer(GBQualifier_value_text_list[2].split(' ')[-1]): + host_age = int(GBQualifier_value_text_list[2].split(' ')[-1]) + + if host_age > -1: + info_for_yaml_dict['host']['host_age'] = host_age + info_for_yaml_dict['host']['host_age_unit'] = 'http://purl.obolibrary.org/obo/UO_0000036' + elif len(GBQualifier_value_text_list) > 2: + missing_value_list.append('\t'.join([accession_version, 'host_age', GBQualifier_value_text_list[2]])) elif GBQualifier_name_text == 'collected_by': if any([x in GBQualifier_value_text.lower() for x in ['institute', 'hospital', 'city', 'center']]): info_for_yaml_dict['sample']['collecting_institution'] = GBQualifier_value_text @@ -362,9 +371,9 @@ for path_metadata_xxx_xml in [os.path.join(dir_metadata, name_metadata_xxx_xml) info_for_yaml_dict['virus']['virus_species'] = "http://purl.obolibrary.org/obo/NCBITaxon_"+GBQualifier_value_text.split('taxon:')[1] - # Remove technology key if empty! - if (info_for_yaml_dict['technology']=={}): - del info_for_yaml_dict['technology'] + if 'sample_sequencing_technology' not in info_for_yaml_dict['technology']: + print(accession_version, ' - technology not found') + continue with open(os.path.join(dir_fasta_and_yaml, '{}.fasta'.format(accession_version)), 'w') as fw: fw.write('>{}\n{}'.format(accession_version, GBSeq_sequence.text.upper())) @@ -391,5 +400,5 @@ if len(accession_with_errors_list) > 0: print('Written the accession with errors in {}'.format(path_accession_with_errors_tsv)) with open(path_accession_with_errors_tsv, 'w') as fw: fw.write('\n'.join(accession_with_errors_list)) - + print('Num. new sequences with length >= {} bp: {}'.format(min_len_to_count, num_seq_with_len_ge_X_bp)) diff --git a/scripts/download_sra_data/SraExperimentPackage.2020.06.08.xml.gz b/scripts/download_sra_data/SraExperimentPackage.2020.06.08.xml.gz Binary files differdeleted file mode 100644 index f9cd995..0000000 --- a/scripts/download_sra_data/SraExperimentPackage.2020.06.08.xml.gz +++ /dev/null diff --git a/scripts/fetch_from_genbank.cwl b/scripts/fetch_from_genbank.cwl new file mode 100644 index 0000000..45c8eec --- /dev/null +++ b/scripts/fetch_from_genbank.cwl @@ -0,0 +1,49 @@ +cwlVersion: v1.1 +class: CommandLineTool +inputs: + importScript: + type: File + default: + class: File + location: download_genbank_data/from_genbank_to_fasta_and_yaml.py + inputBinding: {position: 1} + dict: + type: Directory + inputBinding: + prefix: --dict-ontology + position: 2 + default: + class: Directory + location: dict_ontology_standardization + existing_metadata_from_nuccore: + type: Directory? + inputBinding: + valueFrom: "--skip-request" + position: 3 +outputs: + fasta_and_yaml: + type: Directory + outputBinding: + glob: fasta_and_yaml + metadata_from_nuccore: + type: Directory + outputBinding: + glob: metadata_from_nuccore + accessions: + type: File? + outputBinding: + glob: "*.acc" + missing_terms: + type: File + outputBinding: + glob: missing_terms.tsv +requirements: + InitialWorkDirRequirement: + listing: + - entry: $(inputs.existing_metadata_from_nuccore) + entryname: metadata_from_nuccore + DockerRequirement: + dockerPull: bh20-seq-uploader/import + NetworkAccess: + networkAccess: true +baseCommand: python3 diff --git a/scripts/import.cwl b/scripts/import.cwl index d84516b..4b4b8ca 100644 --- a/scripts/import.cwl +++ b/scripts/import.cwl @@ -12,7 +12,7 @@ inputs: type: File default: class: File - location: from_genbank_to_fasta_and_yaml.py + location: download_genbank_data/from_genbank_to_fasta_and_yaml.py inputBinding: {position: 2} dict: type: Directory diff --git a/scripts/import_from_genbank.cwl b/scripts/import_from_genbank.cwl new file mode 100644 index 0000000..dcf9acb --- /dev/null +++ b/scripts/import_from_genbank.cwl @@ -0,0 +1,27 @@ +cwlVersion: v1.1 +class: Workflow +inputs: + existing_metadata_from_nuccore: + type: Directory? +outputs: [] +requirements: + ScatterFeatureRequirement: {} +steps: + fetch_from_genbank: + in: + existing_metadata_from_nuccore: existing_metadata_from_nuccore + out: [fasta_and_yaml, metadata_from_nuccore, accessions] + run: fetch_from_genbank.cwl + split_into_arrays: + in: + dir: fetch_from_genbank/fasta_and_yaml + out: [fasta, metadata] + run: split_into_arrays.cwl + upload: + in: + fasta: split_into_arrays/fasta + metadata: split_into_arrays/metadata + out: [] + scatter: [fasta, metadata] + scatterMethod: dotproduct + run: upload.cwl diff --git a/scripts/import_to_arvados.py b/scripts/import_to_arvados.py index 78cd13d..aca72d6 100644 --- a/scripts/import_to_arvados.py +++ b/scripts/import_to_arvados.py @@ -11,4 +11,4 @@ os.chdir("fasta_and_yaml") fasta_files = glob.glob("*.fasta") for f in fasta_files: - subprocess.run(["bh20-seq-uploader", f, "%s.yaml" %f[:-6]]) + subprocess.run(["bh20-seq-uploader", "%s.yaml" %f[:-6], f]) diff --git a/scripts/split_into_arrays.cwl b/scripts/split_into_arrays.cwl new file mode 100644 index 0000000..102fe7d --- /dev/null +++ b/scripts/split_into_arrays.cwl @@ -0,0 +1,30 @@ +cwlVersion: v1.1 +class: ExpressionTool +requirements: + InlineJavascriptRequirement: {} +inputs: + dir: + type: Directory + loadListing: shallow_listing +outputs: + fasta: File[] + metadata: File[] +expression: | + ${ + var dir = inputs.dir; + var fasta = []; + var metadata = []; + dir.listing.sort(function(a, b) { return a.basename < b.basename; }); + for (var i = 0; i < dir.listing.length; i++) { + if (dir.listing[i].basename.substr(-6) == ".fasta") { + fasta.push(dir.listing[i]); + } + if (dir.listing[i].basename.substr(-5) == ".yaml") { + metadata.push(dir.listing[i]); + } + } + if (fasta.length != metadata.length) { + throw "They dont match"; + } + return {"fasta": fasta, "metadata": metadata}; + } diff --git a/scripts/upload.cwl b/scripts/upload.cwl new file mode 100644 index 0000000..0fed09a --- /dev/null +++ b/scripts/upload.cwl @@ -0,0 +1,13 @@ +cwlVersion: v1.1 +class: CommandLineTool +inputs: + fasta: File + metadata: File +outputs: [] +requirements: + DockerRequirement: + dockerPull: bh20-seq-uploader/import + NetworkAccess: + networkAccess: true +baseCommand: bh20-seq-uploader +arguments: [--skip-qc, $(inputs.metadata), $(inputs.fasta)] diff --git a/semantic_enrichment/labels.ttl b/semantic_enrichment/labels.ttl index 5d96c5b..68c21bc 100644 --- a/semantic_enrichment/labels.ttl +++ b/semantic_enrichment/labels.ttl @@ -38,6 +38,7 @@ <http://purl.obolibrary.org/obo/OBI_0000759> <http://www.w3.org/2000/01/rdf-schema#label> "Illumina" . <http://purl.obolibrary.org/obo/NCIT_C125894> <http://www.w3.org/2000/01/rdf-schema#label> "Ion Semiconductor Sequencing". <http://purl.obolibrary.org/obo/NCIT_C19641> <http://www.w3.org/2000/01/rdf-schema#label> "Dideoxy Chain Termination DNA Sequencing" . +<http://www.ebi.ac.uk/efo/EFO_0008567> <http://www.w3.org/2000/01/rdf-schema#label> "Illumina HiSeq X" . <http://purl.obolibrary.org/obo/NCIT_C155831> <http://www.w3.org/2000/01/rdf-schema#label> "Nasopharyngeal Swab Specimen" . <http://purl.obolibrary.org/obo/NCIT_C155835> <http://www.w3.org/2000/01/rdf-schema#label> "Oropharyngeal Swab Specimen" . <http://purl.obolibrary.org/obo/NCIT_C13195> <http://www.w3.org/2000/01/rdf-schema#label> "Bronchoalveolar Lavage Fluid" . @@ -46,4 +47,4 @@ <http://purl.obolibrary.org/obo/NCIT_C13234> <http://www.w3.org/2000/01/rdf-schema#label> "Feces" . <http://purl.obolibrary.org/obo/NCIT_C13325> <http://www.w3.org/2000/01/rdf-schema#label> "Serum" . <http://purl.obolibrary.org/obo/NCIT_C13275> <http://www.w3.org/2000/01/rdf-schema#label> "Saliva" . -<http://purl.obolibrary.org/obo/NCIT_C132119> <http://www.w3.org/2000/01/rdf-schema#label> "Nasal Swab" .
\ No newline at end of file +<http://purl.obolibrary.org/obo/NCIT_C132119> <http://www.w3.org/2000/01/rdf-schema#label> "Nasal Swab" . |