diff options
| -rw-r--r-- | scripts/create_sra_metadata/SraExperimentPackage.2020.07.05.xml.gz | bin | 6502056 -> 0 bytes | |||
| -rw-r--r-- | scripts/create_sra_metadata/SraExperimentPackage.2020.07.09.xml.gz | bin | 0 -> 9744133 bytes | |||
| -rw-r--r-- | scripts/create_sra_metadata/create_sra_metadata.py | 62 | ||||
| -rw-r--r-- | scripts/dict_ontology_standardization/ncbi_host_species.csv | 1 | ||||
| -rwxr-xr-x | scripts/download_genbank_data/from_genbank_to_fasta_and_yaml.py | 14 |
5 files changed, 50 insertions, 27 deletions
diff --git a/scripts/create_sra_metadata/SraExperimentPackage.2020.07.05.xml.gz b/scripts/create_sra_metadata/SraExperimentPackage.2020.07.05.xml.gz Binary files differdeleted file mode 100644 index 88acb18..0000000 --- a/scripts/create_sra_metadata/SraExperimentPackage.2020.07.05.xml.gz +++ /dev/null diff --git a/scripts/create_sra_metadata/SraExperimentPackage.2020.07.09.xml.gz b/scripts/create_sra_metadata/SraExperimentPackage.2020.07.09.xml.gz Binary files differnew file mode 100644 index 0000000..93ef550 --- /dev/null +++ b/scripts/create_sra_metadata/SraExperimentPackage.2020.07.09.xml.gz diff --git a/scripts/create_sra_metadata/create_sra_metadata.py b/scripts/create_sra_metadata/create_sra_metadata.py index ef0d119..352a30e 100644 --- a/scripts/create_sra_metadata/create_sra_metadata.py +++ b/scripts/create_sra_metadata/create_sra_metadata.py @@ -8,7 +8,7 @@ import gzip dir_yaml = 'yaml' -date = '2020.07.05' +date = '2020.07.09' # Query on SRA: 'txid2697049[Organism]' (https://www.ncbi.nlm.nih.gov/sra/?term=txid2697049%5BOrganism%5D) # Query on SRA: 'txid2697049[Organism:noexp] NOT 0[Mbases ' (https://www.ncbi.nlm.nih.gov/sra/?term=txid2697049%5BOrganism:noexp%5D%20NOT%200[Mbases) @@ -50,13 +50,14 @@ sra_metadata_xml_file.close() EXPERIMENT_PACKAGE_SET = tree.getroot() missing_value_list = [] +not_created_accession_list = [] run_accession_set = set() run_accession_to_downloadble_file_url_dict = {} for i, EXPERIMENT_PACKAGE in enumerate(EXPERIMENT_PACKAGE_SET): #print(i, EXPERIMENT_PACKAGE) - + # A general default-empty yaml could be read from the definitive one info_for_yaml_dict = { 'id': 'placeholder', @@ -74,17 +75,17 @@ for i, EXPERIMENT_PACKAGE in enumerate(EXPERIMENT_PACKAGE_SET): #print(accession) info_for_yaml_dict['sample']['sample_id'] = accession - + #SRAFiles = RUN.find('SRAFiles') #if SRAFiles is not None: # url = SRAFiles.find('SRAFile').attrib['url'] # if 'sra-download.ncbi.nlm.nih.gov' in url: # run_accession_to_downloadble_file_url_dict[accession] = url - + SAMPLE = EXPERIMENT_PACKAGE.find('SAMPLE') SAMPLE_ATTRIBUTE_list = SAMPLE.iter('SAMPLE_ATTRIBUTE') - + for SAMPLE_ATTRIBUTE in SAMPLE_ATTRIBUTE_list: VALUE = SAMPLE_ATTRIBUTE.find('VALUE') if VALUE is not None: @@ -101,7 +102,7 @@ for i, EXPERIMENT_PACKAGE in enumerate(EXPERIMENT_PACKAGE_SET): missing_value_list.append('\t'.join([accession, 'host_species', VALUE_text])) elif TAG_text in ['host_health_status', 'host health state']: if VALUE_text in term_to_uri_dict: - info_for_yaml_dict['host']['host_health_status'] = term_to_uri_dict[VALUE_text] + info_for_yaml_dict['host']['host_health_status'] = term_to_uri_dict[VALUE_text] elif VALUE_text.strip("'") not in ['missing', 'not collected', 'not provided']: missing_value_list.append('\t'.join([accession, 'host_health_status', VALUE_text])) elif TAG_text in ['strain', 'isolate']: @@ -113,12 +114,12 @@ for i, EXPERIMENT_PACKAGE in enumerate(EXPERIMENT_PACKAGE_SET): if value_to_insert in term_to_uri_dict: value_to_insert = term_to_uri_dict[value_to_insert] - - if 'virus_strain' not in info_for_yaml_dict: + + if 'virus_strain' not in info_for_yaml_dict: info_for_yaml_dict['virus']['virus_strain'] = value_to_insert else: info_for_yaml_dict['virus']['virus_strain'] += '; ' + value_to_insert - elif TAG_text in ['isolation_source', 'isolation source host-associated']: + elif TAG_text in ['isolation_source', 'isolation source host-associated']: if VALUE_text in term_to_uri_dict: info_for_yaml_dict['sample']['specimen_source'] = [term_to_uri_dict[VALUE_text]] else: @@ -145,7 +146,7 @@ for i, EXPERIMENT_PACKAGE in enumerate(EXPERIMENT_PACKAGE_SET): elif TAG_text == 'collected_by': if VALUE_text.lower() not in ['not available', 'missing']: name = VALUE_text in ['Dr. Susie Bartlett', 'Ahmed Babiker', 'Aisi Fu', 'Brandi Williamson', 'George Taiaroa', 'Natacha Ogando', 'Tim Dalebout', 'ykut Ozdarendeli'] - + info_for_yaml_dict['sample']['collector_name' if name else 'collecting_institution'] = VALUE_text elif TAG_text == 'collecting institution': if VALUE_text.lower() not in ['not provided', 'na']: @@ -154,11 +155,11 @@ for i, EXPERIMENT_PACKAGE in enumerate(EXPERIMENT_PACKAGE_SET): if VALUE_text.lower() not in ['not applicable', 'missing', 'na']: date_to_write = VALUE_text date_is_estimated = True - + VALUE_text_list = VALUE_text.split('-') if len(VALUE_text_list) == 3: date_is_estimated = False - + if VALUE_text_list[1].isalpha(): date_to_write = parse(VALUE_text).strftime('%Y-%m-%d') elif len(VALUE_text_list) == 2: @@ -170,7 +171,7 @@ for i, EXPERIMENT_PACKAGE in enumerate(EXPERIMENT_PACKAGE_SET): date_to_write = "{}-01-15".format(VALUE_text) info_for_yaml_dict['sample']['collection_date'] = date_to_write - + if date_is_estimated: if 'additional_collection_information' in info_for_yaml_dict['sample']: info_for_yaml_dict['sample']['additional_collection_information'] += "; The 'collection_date' is estimated (the original date was: {})".format(VALUE_text) @@ -188,8 +189,8 @@ for i, EXPERIMENT_PACKAGE in enumerate(EXPERIMENT_PACKAGE_SET): taxon_id = SAMPLE.find('SAMPLE_NAME').find('TAXON_ID').text info_for_yaml_dict['virus']['virus_species'] = "http://purl.obolibrary.org/obo/NCBITaxon_"+taxon_id - - + + EXPERIMENT = EXPERIMENT_PACKAGE.find('EXPERIMENT') INSTRUMENT_MODEL = [x.text for x in EXPERIMENT.find('PLATFORM').iter('INSTRUMENT_MODEL')][0] @@ -206,18 +207,18 @@ for i, EXPERIMENT_PACKAGE in enumerate(EXPERIMENT_PACKAGE_SET): SUBMISSION = EXPERIMENT_PACKAGE.find('SUBMISSION') info_for_yaml_dict['submitter']['submitter_sample_id'] = SUBMISSION.attrib['accession'] - + if SUBMISSION.attrib['lab_name'].lower() not in ['na']: info_for_yaml_dict['submitter']['originating_lab'] = SUBMISSION.attrib['lab_name'] - STUDY = EXPERIMENT_PACKAGE.find('STUDY') + STUDY = EXPERIMENT_PACKAGE.find('STUDY') info_for_yaml_dict['submitter']['publication'] = STUDY.attrib['alias'] - - + + Organization = EXPERIMENT_PACKAGE.find('Organization') Organization_Name = Organization.find('Name') info_for_yaml_dict['submitter']['authors'] = [Organization_Name.text] - + Organization_Contact = Organization.find('Contact') if Organization_Contact is not None: Organization_Contact_Name = Organization_Contact.find('Name') @@ -231,20 +232,33 @@ for i, EXPERIMENT_PACKAGE in enumerate(EXPERIMENT_PACKAGE_SET): Organization_Address = Organization.find('Address') if Organization_Address is not None: info_for_yaml_dict['submitter']['lab_address'] = '; '.join([x.text for x in Organization_Address] + ['Postal code ' + Organization_Address.attrib['postal_code']]) - + if 'collection_date' not in info_for_yaml_dict['sample']: info_for_yaml_dict['sample']['collection_date'] = '1970-01-01' info_for_yaml_dict['sample']['additional_collection_information'] = "The real 'collection_date' is missing" if 'sample_sequencing_technology' not in info_for_yaml_dict['technology']: - print(accession, ' - technology not found') + #print(accession, ' - technology not found') + not_created_accession_list.append([accession, 'technology not found']) + continue + + if 'host_species' not in info_for_yaml_dict['host']: + #print(accession, ' - technology not found') + not_created_accession_list.append([accession, 'missing host species']) continue with open(os.path.join(dir_yaml, '{}.yaml'.format(accession)), 'w') as fw: json.dump(info_for_yaml_dict, fw, indent=2) - + if len(missing_value_list) > 0: - path_missing_terms_tsv = 'missing_terms.tsv' + path_missing_terms_tsv = 'missing_terms.sra.tsv' print('Written missing terms in {}'.format(path_missing_terms_tsv)) with open(path_missing_terms_tsv, 'w') as fw: fw.write('\n'.join(missing_value_list)) + +if len(not_created_accession_list) > 0: + path_not_created_accession_tsv = 'not_created_accession.sra.tsv' + print('Written not created accession in {}'.format(path_not_created_accession_tsv)) + with open(path_not_created_accession_tsv, 'w') as fw: + fw.write('\n'.join(['\t'.join(x) for x in not_created_accession_list])) + diff --git a/scripts/dict_ontology_standardization/ncbi_host_species.csv b/scripts/dict_ontology_standardization/ncbi_host_species.csv index 40572a3..0bfc455 100644 --- a/scripts/dict_ontology_standardization/ncbi_host_species.csv +++ b/scripts/dict_ontology_standardization/ncbi_host_species.csv @@ -2,6 +2,7 @@ Homo sapiens,http://purl.obolibrary.org/obo/NCBITaxon_9606 human,http://purl.obolibrary.org/obo/NCBITaxon_9606 Human,http://purl.obolibrary.org/obo/NCBITaxon_9606 sapiens,http://purl.obolibrary.org/obo/NCBITaxon_9606 +homosapiens,http://purl.obolibrary.org/obo/NCBITaxon_9606 Mustela lutreola,http://purl.obolibrary.org/obo/NCBITaxon_9666 Manis javanica,http://purl.obolibrary.org/obo/NCBITaxon_9974 Felis catus,http://purl.obolibrary.org/obo/NCBITaxon_9685 diff --git a/scripts/download_genbank_data/from_genbank_to_fasta_and_yaml.py b/scripts/download_genbank_data/from_genbank_to_fasta_and_yaml.py index 39e401a..dbebfbb 100755 --- a/scripts/download_genbank_data/from_genbank_to_fasta_and_yaml.py +++ b/scripts/download_genbank_data/from_genbank_to_fasta_and_yaml.py @@ -138,6 +138,7 @@ min_len_to_count = 27500 num_seq_with_len_ge_X_bp = 0 missing_value_list = [] +not_created_accession_list = [] accession_with_errors_list = [] for path_metadata_xxx_xml in [os.path.join(dir_metadata, name_metadata_xxx_xml) for name_metadata_xxx_xml in os.listdir(dir_metadata) if name_metadata_xxx_xml.endswith('.xml')]: @@ -371,7 +372,8 @@ for path_metadata_xxx_xml in [os.path.join(dir_metadata, name_metadata_xxx_xml) if 'sample_sequencing_technology' not in info_for_yaml_dict['technology']: - print(accession_version, ' - technology not found') + #print(accession_version, ' - technology not found') + not_created_accession_list.append([accession_version, 'technology not found']) continue with open(os.path.join(dir_fasta_and_yaml, '{}.fasta'.format(accession_version)), 'w') as fw: @@ -389,15 +391,21 @@ for path_metadata_xxx_xml in [os.path.join(dir_metadata, name_metadata_xxx_xml) continue if len(missing_value_list) > 0: - path_missing_terms_tsv = 'missing_terms.tsv' + path_missing_terms_tsv = 'missing_terms.genbank.tsv' print('Written missing terms in {}'.format(path_missing_terms_tsv)) with open(path_missing_terms_tsv, 'w') as fw: fw.write('\n'.join(missing_value_list)) if len(accession_with_errors_list) > 0: - path_accession_with_errors_tsv = 'accession_with_errors.tsv' + path_accession_with_errors_tsv = 'accession_with_errors.genbank.tsv' print('Written the accession with errors in {}'.format(path_accession_with_errors_tsv)) with open(path_accession_with_errors_tsv, 'w') as fw: fw.write('\n'.join(accession_with_errors_list)) +if len(not_created_accession_list) > 0: + path_not_created_accession_tsv = 'not_created_accession.genbank.tsv' + print('Written not created accession in {}'.format(path_not_created_accession_tsv)) + with open(path_not_created_accession_tsv, 'w') as fw: + fw.write('\n'.join(['\t'.join(x) for x in not_created_accession_list])) + print('Num. new sequences with length >= {} bp: {}'.format(min_len_to_count, num_seq_with_len_ge_X_bp)) |