Merge pull request #117 from arvados/pangenome_workflow_abpoa

Pangenome workflow with abPOA

1 files changed, 120 insertions, 0 deletions

diff --git a/workflows/pangenome-generate/pangenome-generate_abpoa.cwl b/workflows/pangenome-generate/pangenome-generate_abpoa.cwl
new file mode 100644
index 0000000..c61e51a
--- /dev/null
+++ b/workflows/pangenome-generate/pangenome-generate_abpoa.cwl
@@ -0,0 +1,120 @@
+#!/usr/bin/env cwl-runner
+cwlVersion: v1.1
+class: Workflow
+requirements:
+  ScatterFeatureRequirement: {}
+  StepInputExpressionRequirement: {}
+inputs:
+  seqs: File
+  metadata: File
+  bin_widths:
+    type: int[]
+    default: [ 1, 4, 16, 64, 256, 1000, 4000, 16000]
+    doc: width of each bin in basepairs along the graph vector
+  cells_per_file:
+    type: int
+    default: 100
+    doc: Cells per file on component_segmentation
+  reversed_sort:
+    type: string
+    default: "false"
+outputs:
+  odgiGraph:
+    type: File
+    outputSource: buildGraph/odgiGraph
+  odgiPNG:
+   type: File
+   outputSource: vizGraph/graph_image
+  abpoaGFA:
+    type: File
+    outputSource: induceGraph/abpoaGFA
+#  odgiRDF:
+#    type: File
+#    outputSource: odgi2rdf/rdf
+  readsMergeDedupSortedByQualAndLen:
+    type: File
+    outputSource: dedup_and_sort_by_quality_and_len/sortedReadsFA
+  mergedMetadata:
+    type: File
+    outputSource: dups2metadata/merged
+#  indexed_paths:
+#    type: File
+#    outputSource: index_paths/indexed_paths
+#  colinear_components:
+#    type: Directory
+#    outputSource: segment_components/colinear_components
+steps:
+  dedup_and_sort_by_quality_and_len:
+    in: {readsFA: seqs, reversed_sorting: reversed_sort}
+    out: [sortedReadsFA, dups]
+    run: sort_fasta_by_quality_and_len.cwl
+  induceGraph:
+    in:
+      readsFA: dedup_and_sort_by_quality_and_len/sortedReadsFA
+    out: [abpoaGFA]
+    run: abpoa.cwl
+  buildGraph:
+    in: {inputGFA: induceGraph/abpoaGFA}
+    out: [odgiGraph]
+    run: odgi-build-from-xpoa-gfa.cwl
+  vizGraph:
+    in:
+      sparse_graph_index: buildGraph/odgiGraph
+      width:
+        default: 50000
+      height:
+        default: 500
+      path_per_row:
+        default: true
+      path_height:
+        default: 4
+    out: [graph_image]
+    requirements:
+      ResourceRequirement:
+        ramMin: $(15 * 1024)
+        outdirMin: 10
+    run: ../tools/odgi/odgi_viz.cwl
+  # odgi2rdf:
+  #  in: {odgi: buildGraph/odgiGraph}
+  #  out: [rdf]
+  #  run: odgi_to_rdf.cwl
+  dups2metadata:
+    in:
+      metadata: metadata
+      dups: dedup_and_sort_by_quality_and_len/dups
+    out: [merged]
+    run: dups2metadata.cwl
+  # bin_paths:
+  #   requirements:
+  #     ResourceRequirement:
+  #       ramMin: 3000
+  #       outdirMin: 10
+  #   run: ../tools/odgi/odgi_bin.cwl
+  #   in:
+  #     sparse_graph_index: buildGraph/odgiGraph
+  #     bin_width: bin_widths
+  #   scatter: bin_width
+  #   out: [ bins, pangenome_sequence ]
+  # index_paths:
+  #   label: Create path index
+  #   requirements:
+  #     ResourceRequirement:
+  #       ramMin: 3000
+  #       outdirMin: 10
+  #   run: ../tools/odgi/odgi_pathindex.cwl
+  #   in:
+  #     sparse_graph_index: buildGraph/odgiGraph
+  #   out: [ indexed_paths ]
+  # segment_components:
+  #   label: Run component segmentation
+  #   run: ../tools/graph-genome-segmentation/component_segmentation.cwl
+  #   in:
+  #     bins: bin_paths/bins
+  #     cells_per_file: cells_per_file
+  #     pangenome_sequence:
+  #       source: bin_paths/pangenome_sequence
+  #       valueFrom: $(self[0])
+  #       # the bin_paths step is scattered over the bin_width array, but always using the same sparse_graph_index
+  #       # the pangenome_sequence that is extracted is exactly the same for the same sparse_graph_index
+  #       # regardless of bin_width, so we take the first pangenome_sequence as input for this step
+  #   out: [ colinear_components ]