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authorPeter Amstutz2020-04-20 12:55:18 -0400
committerPeter Amstutz2020-04-20 12:55:18 -0400
commitd781e42c9adac07253cb928ae66e9b7314710267 (patch)
tree4718165cbc069169eaff98080c61c5f07763ffc1 /workflows/fastq2fasta/bam2fasta.cwl
parent1219eaf496c899f3043b90e30eb956f0f363bfb3 (diff)
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Move workflows into main repo
Arvados-DCO-1.1-Signed-off-by: Peter Amstutz <peter.amstutz@curii.com>
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+# Reference:
+# https://github.com/VGP/vgp-assembly/blob/33cd6236a68a1aee5f282e365dfe6b97e0b4ebb7/pipeline/freebayes-polish/freebayes.sh
+# https://github.com/VGP/vgp-assembly/blob/33cd6236a68a1aee5f282e365dfe6b97e0b4ebb7/pipeline/freebayes-polish/consensus.sh
+class: Workflow
+cwlVersion: v1.1
+id: bam2fasta
+label: bam2fasta
+requirements: []
+
+inputs:
+ bam:
+ type: File
+ fasta:
+ type: File
+ threads:
+ type: int
+ default: 4
+
+outputs:
+ out_fasta:
+ type: File
+ outputSource: bcftools_consensus/out_fasta
+
+steps:
+ freebayes:
+ in:
+ bam: bam
+ ref_fasta: fasta
+ out: [vcf]
+ run: freebayes.cwl
+ bcftools_view_exclude_ref:
+ in:
+ vcf: freebayes/vcf
+ threads: threads
+ out: [bcf]
+ run: bcftools-view-exclude-ref.cwl
+ bcftools_norm:
+ in:
+ ref_fasta: fasta
+ bcf: bcftools_view_exclude_ref/bcf
+ threads: threads
+ out: [normalized_bcf]
+ run: bcftools-norm.cwl
+ bcftools_index_after_normalization:
+ in:
+ bcf: bcftools_norm/normalized_bcf
+ out: [indexed]
+ run: bcftools-index.cwl
+ bcftools_view_qc:
+ in:
+ bcf: bcftools_index_after_normalization/indexed
+ threads: threads
+ out: [vcf]
+ run: bcftools-view-qc.cwl
+ bcftools_index_after_qc:
+ in:
+ bcf: bcftools_view_qc/vcf
+ out: [indexed]
+ run: bcftools-index.cwl
+ bcftools_consensus:
+ in:
+ ref_fasta: fasta
+ vcf: bcftools_index_after_qc/indexed
+ out: [out_fasta]
+ run: bcftools-consensus.cwl