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authorPjotr Prins2020-05-30 18:13:48 -0500
committerPjotr Prins2020-05-30 18:13:48 -0500
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+<title>COVID-19 PubSeq (part 2)</title>
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+<meta name="author" content="Pjotr Prins" />
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+</style>
+<link rel="Blog stylesheet" type="text/css" href="blog.css" />
+<script type="text/javascript">
+/*
+@licstart The following is the entire license notice for the
+JavaScript code in this tag.
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+
+The JavaScript code in this tag is free software: you can
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+any later version. The code is distributed WITHOUT ANY WARRANTY;
+without even the implied warranty of MERCHANTABILITY or FITNESS
+FOR A PARTICULAR PURPOSE. See the GNU GPL for more details.
+
+As additional permission under GNU GPL version 3 section 7, you
+may distribute non-source (e.g., minimized or compacted) forms of
+that code without the copy of the GNU GPL normally required by
+section 4, provided you include this license notice and a URL
+through which recipients can access the Corresponding Source.
+
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+ target.className = elem.cacheClassTarget;
+ }
+/*]]>*///-->
+</script>
+</head>
+<body>
+<div id="org-div-home-and-up">
+ <a accesskey="h" href=""> UP </a>
+ |
+ <a accesskey="H" href="http://covid19.genenetwork.org"> HOME </a>
+</div><div id="content">
+<h1 class="title">COVID-19 PubSeq (part 2)</h1>
+<div id="table-of-contents">
+<h2>Table of Contents</h2>
+<div id="text-table-of-contents">
+<ul>
+<li><a href="#org7942167">1. Finding output of workflows</a></li>
+<li><a href="#org0022bbe">2. Introduction</a></li>
+<li><a href="#org3929710">3. The Arvados file interface</a></li>
+<li><a href="#orgc4dba6e">4. Using the Arvados API</a></li>
+</ul>
+</div>
+</div>
+<p>
+As part of the COVID-19 Biohackathon 2020 we formed a working group to
+create a COVID-19 Public Sequence Resource (COVID-19 PubSeq) for
+Corona virus sequences. The general idea is to create a repository
+that has a low barrier to entry for uploading sequence data using best
+practices. I.e., data published with a creative commons 4.0 (CC-4.0)
+license with metadata using state-of-the art standards and, perhaps
+most importantly, providing standardised workflows that get triggered
+on upload, so that results are immediately available in standardised
+data formats.
+</p>
+
+<div id="outline-container-org7942167" class="outline-2">
+<h2 id="org7942167"><span class="section-number-2">1</span> Finding output of workflows</h2>
+<div class="outline-text-2" id="text-1">
+<p>
+As part of the COVID-19 Biohackathon 2020 we formed a working group to
+create a COVID-19 Public Sequence Resource (COVID-19 PubSeq) for
+Corona virus sequences. The general idea is to create a repository
+that has a low barrier to entry for uploading sequence data using best
+practices. I.e., data published with a creative commons 4.0 (CC-4.0)
+license with metadata using state-of-the art standards and, perhaps
+most importantly, providing standardised workflows that get triggered
+on upload, so that results are immediately available in standardised
+data formats.
+</p>
+</div>
+</div>
+
+<div id="outline-container-org0022bbe" class="outline-2">
+<h2 id="org0022bbe"><span class="section-number-2">2</span> Introduction</h2>
+<div class="outline-text-2" id="text-2">
+<p>
+We are using Arvados to run common workflow language (CWL) pipelines.
+The most recent output is on display on a <a href="https://workbench.lugli.arvadosapi.com/collections/lugli-4zz18-z513nlpqm03hpca">web page</a> (with time stamp)
+and a full list is generated <a href="https://collections.lugli.arvadosapi.com/c=lugli-4zz18-z513nlpqm03hpca/">here</a>. It is nice to start up, but for
+most users we need a dedicated and themed results page. People don't
+want to wade through thousands of output files!
+</p>
+</div>
+</div>
+
+<div id="outline-container-org3929710" class="outline-2">
+<h2 id="org3929710"><span class="section-number-2">3</span> The Arvados file interface</h2>
+<div class="outline-text-2" id="text-3">
+<p>
+Arvados has the web server, but it also has a REST API and associated
+command line tools. We are already using the <a href="https://github.com/arvados/bh20-seq-resource/blob/master/bh20sequploader/main.py#L27">API</a> to upload data. If
+you follow the pip or <a href="../INSTALL.md">../INSTALL.md</a> GNU Guix instructions for
+installing Arvados API you'll find the following command line tools
+(also documented <a href="https://doc.arvados.org/v2.0/sdk/cli/subcommands.html">here</a>):
+</p>
+
+<table border="2" cellspacing="0" cellpadding="6" rules="groups" frame="hsides">
+
+
+<colgroup>
+<col class="org-left" />
+
+<col class="org-left" />
+</colgroup>
+<thead>
+<tr>
+<th scope="col" class="org-left">Command</th>
+<th scope="col" class="org-left">Description</th>
+</tr>
+</thead>
+<tbody>
+<tr>
+<td class="org-left">arv-ls</td>
+<td class="org-left">list files in Arvados</td>
+</tr>
+
+<tr>
+<td class="org-left">arv-put</td>
+<td class="org-left">upload a file to Arvados</td>
+</tr>
+
+<tr>
+<td class="org-left">arv-get</td>
+<td class="org-left">get a textual representation of Arvados objects from the command line. The output can be limited to a subset of the object’s fields. This command can be used with only the knowledge of an object’s UUID</td>
+</tr>
+</tbody>
+</table>
+
+<p>
+Now, this is a public instance so we can use the tokens from
+the <a href="https://github.com/arvados/bh20-seq-resource/blob/master/bh20sequploader/main.py#L16">uploader</a>.
+</p>
+
+<div class="SOURCE">
+<p>
+export ARVADOS<sub>API</sub><sub>HOST</sub>='lugli.arvadosapi.com'
+export ARVADOS<sub>API</sub><sub>TOKEN</sub>='2fbebpmbo3rw3x05ueu2i6nx70zhrsb1p22ycu3ry34m4x4462'
+arv-ls lugli-4zz18-z513nlpqm03hpca
+</p>
+
+</div>
+
+<p>
+will list all files (the UUID we got from the Arvados results page). To
+get the UUID of the files
+</p>
+
+<div class="SOURCE">
+<p>
+curl <a href="https://lugli.arvadosapi.com/arvados/v1/config">https://lugli.arvadosapi.com/arvados/v1/config</a> | jq .Users.AnonymousUserToken
+env ARVADOS<sub>API</sub><sub>TOKEN</sub>=5o42qdxpxp5cj15jqjf7vnxx5xduhm4ret703suuoa3ivfglfh \
+ arv-get lugli-4zz18-z513nlpqm03hpca
+</p>
+
+</div>
+
+<p>
+and fetch one listed JSON file <code>chunk001_bin4000.schematic.json</code> with
+its listed UUID:
+</p>
+
+<pre class="example">
+arv-get 2be6af7b4741f2a5c5f8ff2bc6152d73+1955623+Ab9ad65d7fe958a053b3a57d545839de18290843a@5ed7f3c5
+</pre>
+</div>
+</div>
+
+<div id="outline-container-orgc4dba6e" class="outline-2">
+<h2 id="orgc4dba6e"><span class="section-number-2">4</span> Using the Arvados API</h2>
+</div>
+</div>
+<div id="postamble" class="status">
+<hr><small>Created by <a href="http://thebird.nl/">Pjotr Prins</a> (pjotr.public768 at thebird 'dot' nl) using Emacs org-mode and a healthy dose of Lisp!<br />Modified 2020-05-30 Sat 11:50</small>.
+</div>
+</body>
+</html>
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