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import argparse
import time
import arvados
import arvados.collection
import json
import logging
import magic
from pathlib import Path
import urllib.request
import socket
import getpass
import sys
sys.path.insert(0,'.')
from bh20sequploader.qc_metadata import qc_metadata
from bh20sequploader.qc_fasta import qc_fasta
logging.basicConfig(level=logging.DEBUG)
log = logging.getLogger(__name__ )
log.debug("Entering sequence uploader")
ARVADOS_API_HOST='lugli.arvadosapi.com'
ARVADOS_API_TOKEN='2fbebpmbo3rw3x05ueu2i6nx70zhrsb1p22ycu3ry34m4x4462'
UPLOAD_PROJECT='lugli-j7d0g-n5clictpuvwk8aa'
def main():
parser = argparse.ArgumentParser(description='Upload SARS-CoV-19 sequences for analysis')
parser.add_argument('sequence', type=argparse.FileType('r'), help='sequence FASTA/FASTQ')
parser.add_argument('metadata', type=argparse.FileType('r'), help='sequence metadata json')
parser.add_argument("--validate", action="store_true", help="Dry run, validate only")
args = parser.parse_args()
api = arvados.api(host=ARVADOS_API_HOST, token=ARVADOS_API_TOKEN, insecure=True)
log.debug("Checking metadata")
if not qc_metadata(args.metadata.name):
log.warning("Failed metadata qc")
exit(1)
try:
log.debug("Checking FASTA QC")
target = qc_fasta(args.sequence)
except ValueError as e:
log.warning("Failed FASTA qc")
print(e)
exit(1)
if args.validate:
print("Valid")
exit(0)
col = arvados.collection.Collection(api_client=api)
with col.open(target, "w") as f:
r = args.sequence.read(65536)
seqlabel = r[1:r.index("\n")]
print(seqlabel)
while r:
f.write(r)
r = args.sequence.read(65536)
args.sequence.close()
print("Reading metadata")
with col.open("metadata.yaml", "w") as f:
r = args.metadata.read(65536)
print(r[0:20])
while r:
f.write(r)
r = args.metadata.read(65536)
args.metadata.close()
external_ip = urllib.request.urlopen('https://ident.me').read().decode('utf8')
try:
username = getpass.getuser()
except KeyError:
username = "unknown"
properties = {
"sequence_label": seqlabel,
"upload_app": "bh20-seq-uploader",
"upload_ip": external_ip,
"upload_user": "%s@%s" % (username, socket.gethostname())
}
col.save_new(owner_uuid=UPLOAD_PROJECT, name="%s uploaded by %s from %s" %
(seqlabel, properties['upload_user'], properties['upload_ip']),
properties=properties, ensure_unique_name=True)
print("Done")
if __name__ == "__main__":
main()
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