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diff --git a/paper/paper.md b/paper/paper.md index caa9903..48eac07 100644 --- a/paper/paper.md +++ b/paper/paper.md @@ -1,8 +1,9 @@ --- -title: 'Public Sequence Resource for COVID-19' +title: 'CPSR: COVID-19 Public Sequence Resource' +title_short: 'CPSR: COVID-19 Public Sequence Resource' tags: - Sequencing - - COVID + - COVID-19 authors: - name: Pjotr Prins orcid: 0000-0002-8021-9162 @@ -19,22 +20,45 @@ authors: - name: Erik Garrison orcid: 0000 affiliation: 5 - - name: Michael Crusoe - orcid: 0000 - affiliation: 6 + - name: Michael R. Crusoe + orcid: 0000-0002-2961-9670 + affiliation: 6, 2 - name: Rutger Vos orcid: 0000 affiliation: 7 - - Michael Heuer - orcid: 0000 + - name: Michael Heuer + orcid: 0000-0002-9052-6000 affiliation: 8 - + - name: Adam M Novak + orcid: 0000-0001-5828-047X + affiliation: 5 + - name: Alex Kanitz + orcid: 0000 + affiliation: 10 + - name: Jerven Bolleman + orcid: 0000 + affiliation: 11 + - name: Joep de Ligt + orcid: 0000 + affiliation: 12 + - name: Bonface Munyoki + orcid: 0000 + affiliation: 13 affiliations: - name: Department of Genetics, Genomics and Informatics, The University of Tennessee Health Science Center, Memphis, TN, USA. index: 1 - name: Curii, Boston, USA index: 2 + - name: UC Santa Cruz Genomics Institute, University of California, Santa Cruz, CA 95064, USA. + index: 5 + - name: Department of Computer Science, Faculty of Sciences, Vrije Universiteit Amsterdam, The Netherlands + index: 6 + - name: RISE Lab, University of California Berkeley, Berkeley, CA, USA. + index: 8 date: 11 April 2020 +event: COVID2020 +group: Public Sequence Uploader +authors_short: Pjotr Prins & Peter Amstutz \emph{et al.} bibliography: paper.bib --- @@ -49,13 +73,48 @@ pasting above link (or yours) with https://github.com/biohackrxiv/bhxiv-gen-pdf +Note that author order will change! + --> # Introduction -As part of the one week COVID-19 Biohackathion 2020, we formed a -working group on creating a public sequence resource for Corona virus. - +As part of the COVID-19 Biohackathion 2020 we formed a working +group to create a COVID-19 Public Sequence Resource (CPSR) for +Corona virus sequences. The general idea was to create a +repository that has a low barrier to entry for uploading sequence +data using best practices. I.e., data published with a creative +commons 4.0 (CC-4.0) license with metadata using state-of-the art +standards and, perhaps most importantly, providing standardized +workflows that get triggered on upload, so that results are +immediately available in standardized data formats. + +Existing data repositories for viral data include GISAID, EBI ENA +and NCBI. These repositories allow for free sharing of data, but +do not add value in terms of running immediate +computations. Also, GISAID, at this point, has the most complete +collection of genetic sequence data of influenza viruses and +related clinical and epidemiological data through its +database. But, due to a restricted license, data submitted to +GISAID can not be used for online web services and on-the-fly +computation. In addition GISAID registration which can take weeks +and, painfully, forces users to download sequences one at a time +to do any type of analysis. In our opinion this does not fit a +pandemic scenario where fast turnaround times are key and data +analysis has to be agile. + +We managed to create a useful sequence uploader utility within +one week by leveraging existing technologies, such as the Arvados +Cloud platform [@Arvados], the Common Workflow Langauge (CWL) +[@CWL], Docker images built with Debian packages, and the many +free and open source software packages that are available for +bioinformatics. + +The source code for the CLI uploader and web uploader can be +found [here](https://github.com/arvados/bh20-seq-resource) +(FIXME: we'll have a full page). The CWL workflow definitions can +be found [here](https://github.com/hpobio-lab/viral-analysis) and +on CWL hub (FIXME). <!-- @@ -73,38 +132,98 @@ working group on creating a public sequence resource for Corona virus. ## Cloud computing backend -Peter, Pjotr, MichaelC +The development of CPSR was accelerated by using the Arvados +Cloud platform. Arvados is an open source platform for managing, +processing, and sharing genomic and other large scientific and +biomedical data. The Arvados instance was deployed on Amazon AWS +for testing and development and a project was created that +allows for uploading data. -## A command-line sequence uploader +## Sequence uploader -Peter, Pjotr +We wrote a Python-based uploader that authenticates with Arvados +using a token. Data gets validated for being a FASTA sequence, +FASTQ raw data and/or metadata in the form of JSON LD that gets +validated against a schema. The uploader can be used +from a command line or using a simple web interface. -## Metadata uploader +## Creating a Pangenome -With Thomas +### FASTA to GFA workflow -## FASTA to GFA workflow +The first workflow (1) we implemented was a FASTA to Graphical +Fragment Assembly (GFA) Format conversion. When someone uploads a +sequence in FASTA format it gets combined with all known viral +sequences in our storage to generate a pangenome or variation +graph (VG). The full pangenome is made available as a +downloadable GFA file together with a visualisation (Figure 1). -Michael Heuer +### FASTQ to GFA workflow -## BAM to GFA workflow +In the next step we introduced a workflow (2) that takes raw +sequence data in fastq format and converts that into FASTA. +This FASTA file, in turn, gets fed to workflow (1) to generate +the pangenome. -Tazro & Erik +## Creating linked data workflow -## Phylogeny app +We created a workflow (3) that takes GFA and turns that into +RDF. Together with the metadata at upload time a single RDF +resource is compiled that can be linked against external +resources such as Uniprot and Wikidata. The generated RDF file +can be hosted in any triple store and queried using SPARQL. -With Rutger +## Creating a Phylogeny workflow -## RDF app +WIP -Jerven? - -## EBI app - -? +## Other workflows? # Discussion -Future work... +CPSR is a data repository with computational pipelines that will +persist during pandemics. Unlike other data repositories for +Sars-COV-2 we created a repository that immediately computes the +pangenome of all available data and presents that in useful +formats for futher analysis, including visualisations, GFA and +RDF. Code and data are available and written using best practises +and state-of-the-art standards. CPSR can be deployed by anyone, +anywhere. + +CPSR is designed to abide by FAIR data principles (expand...) + +CPSR is primed with viral data coming from repositories that have +no sharing restrictions. The metadata includes relevant +attribution to uploaders. Some institutes have already committed +to uploading their data to CPSR first so as to warrant sharing +for computation. + +CPSR is currently running on an Arvados cluster in the cloud. To +ascertain the service remains running we will source money from +project during pandemics. The workflows are written in CWL which +means they can be deployed on any infrastructure that runs +CWL. One of the advantages of the CC-4.0 license is that we make +available all uploaded sequence and meta data, as well as +results, online to anyone. So the data can be mirrored by any +party. This guarantees the data will live on. + +<!-- Future work... --> + +We aim to add more workflows to CPSR, for example to prepare +sequence data for submitting in other public repositories, such +as EBI ENA and GISAID. This will allow researchers to share data +in multiple systems without pain, circumventing current sharing +restrictions. + +# Acknowledgements + +We thank the COVID-19 BioHackathon 2020 and ELIXIR for creating a +unique event that triggered many collaborations. We thank Curii +Corporation for their financial support for creating and running +Arvados instances. We thank Amazon AWS for their financial +support to run COVID-19 workflows. We also want to thank the +other working groups in the BioHackathon who generously +contributed onthologies, workflows and software. + # References |