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Diffstat (limited to 'doc/web/about.html')
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1 files changed, 952 insertions, 537 deletions
diff --git a/doc/web/about.html b/doc/web/about.html index dfd4252..c971a4e 100644 --- a/doc/web/about.html +++ b/doc/web/about.html @@ -1,549 +1,964 @@ <?xml version="1.0" encoding="utf-8"?> <!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Strict//EN" -"http://www.w3.org/TR/xhtml1/DTD/xhtml1-strict.dtd"> + "http://www.w3.org/TR/xhtml1/DTD/xhtml1-strict.dtd"> <html xmlns="http://www.w3.org/1999/xhtml" lang="en" xml:lang="en"> <head> -<!-- 2020-07-18 Sat 03:27 --> -<meta http-equiv="Content-Type" content="text/html;charset=utf-8" /> -<meta name="viewport" content="width=device-width, initial-scale=1" /> -<title>About/FAQ</title> -<meta name="generator" content="Org mode" /> -<meta name="author" content="Pjotr Prins" /> -<style type="text/css"> - <!--/*--><![CDATA[/*><!--*/ - .title { text-align: center; - margin-bottom: .2em; } - .subtitle { text-align: center; - font-size: medium; - font-weight: bold; - margin-top:0; } - .todo { font-family: monospace; color: red; } - .done { font-family: monospace; 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See the GNU GPL for more details. - -As additional permission under GNU GPL version 3 section 7, you -may distribute non-source (e.g., minimized or compacted) forms of -that code without the copy of the GNU GPL normally required by -section 4, provided you include this license notice and a URL -through which recipients can access the Corresponding Source. - - -@licend The above is the entire license notice -for the JavaScript code in this tag. -*/ -<!--/*--><![CDATA[/*><!--*/ - function CodeHighlightOn(elem, id) - { - var target = document.getElementById(id); - if(null != target) { - elem.cacheClassElem = elem.className; - elem.cacheClassTarget = target.className; - target.className = "code-highlighted"; - elem.className = "code-highlighted"; - } - } - function CodeHighlightOff(elem, id) - { - var target = document.getElementById(id); - if(elem.cacheClassElem) - elem.className = elem.cacheClassElem; - if(elem.cacheClassTarget) - target.className = elem.cacheClassTarget; - } -/*]]>*///--> -</script> + <!-- 2020-07-18 Sat 03:27 --> + <meta http-equiv="Content-Type" content="text/html;charset=utf-8"/> + <meta name="viewport" content="width=device-width, initial-scale=1"/> + <title>About/FAQ</title> + <meta name="generator" content="Org mode"/> + <meta name="author" content="Pjotr Prins"/> + <style type="text/css"> + <!-- /*--><![CDATA[/*><!--*/ + .title { + text-align: center; + margin-bottom: .2em; + } + + .subtitle { + text-align: center; + font-size: medium; + font-weight: bold; + margin-top: 0; + } + + .todo { + font-family: monospace; + color: red; + } + + .done { + font-family: monospace; + color: green; + } + + .priority { + font-family: monospace; + color: orange; + } + + .tag { + background-color: #eee; + font-family: monospace; + padding: 2px; + font-size: 80%; + font-weight: normal; + } + + .timestamp { + color: #bebebe; + } + + .timestamp-kwd { + color: #5f9ea0; + } + + .org-right { + margin-left: auto; + margin-right: 0px; + text-align: right; + } + + .org-left { + margin-left: 0px; + margin-right: auto; + text-align: left; + } + + .org-center { + margin-left: auto; + margin-right: auto; + text-align: center; + } + + .underline { + text-decoration: underline; + } + + #postamble p, #preamble p { + font-size: 90%; + margin: .2em; + } + + p.verse { + margin-left: 3%; + } + + pre { + border: 1px solid #ccc; + box-shadow: 3px 3px 3px #eee; + padding: 8pt; + font-family: monospace; + overflow: auto; + margin: 1.2em; + } + + pre.src { + position: relative; + overflow: visible; + padding-top: 1.2em; + } + + pre.src:before { + display: none; + position: absolute; + background-color: white; + top: -10px; + right: 10px; + padding: 3px; + border: 1px solid black; + } + + pre.src:hover:before { + display: inline; + } + + /* Languages per Org manual */ + pre.src-asymptote:before { + content: 'Asymptote'; + } + + pre.src-awk:before { + content: 'Awk'; + } + + pre.src-C:before { + content: 'C'; + } + + /* pre.src-C++ doesn't work in CSS */ + pre.src-clojure:before { + content: 'Clojure'; + } + + pre.src-css:before { + content: 'CSS'; + } + + pre.src-D:before { + content: 'D'; + } + + pre.src-ditaa:before { + content: 'ditaa'; + } + + pre.src-dot:before { + content: 'Graphviz'; + } + + pre.src-calc:before { + content: 'Emacs Calc'; + } + + pre.src-emacs-lisp:before { + content: 'Emacs Lisp'; + } + + pre.src-fortran:before { + content: 'Fortran'; + } + + pre.src-gnuplot:before { + content: 'gnuplot'; + } + + pre.src-haskell:before { + content: 'Haskell'; + } + + pre.src-hledger:before { + content: 'hledger'; + } + + pre.src-java:before { + content: 'Java'; + } + + pre.src-js:before { + content: 'Javascript'; + } + + pre.src-latex:before { + content: 'LaTeX'; + } + + pre.src-ledger:before { + content: 'Ledger'; + } + + pre.src-lisp:before { + content: 'Lisp'; + } + + pre.src-lilypond:before { + content: 'Lilypond'; + } + + pre.src-lua:before { + content: 'Lua'; + } + + pre.src-matlab:before { + content: 'MATLAB'; + } + + pre.src-mscgen:before { + content: 'Mscgen'; + } + + pre.src-ocaml:before { + content: 'Objective Caml'; + } + + pre.src-octave:before { + content: 'Octave'; + } + + pre.src-org:before { + content: 'Org mode'; + } + + pre.src-oz:before { + content: 'OZ'; + } + + pre.src-plantuml:before { + content: 'Plantuml'; + } + + pre.src-processing:before { + content: 'Processing.js'; + } + + pre.src-python:before { + content: 'Python'; + } + + pre.src-R:before { + content: 'R'; + } + + pre.src-ruby:before { + content: 'Ruby'; + } + + pre.src-sass:before { + content: 'Sass'; + } + + pre.src-scheme:before { + content: 'Scheme'; + } + + pre.src-screen:before { + content: 'Gnu Screen'; + } + + pre.src-sed:before { + content: 'Sed'; + } + + pre.src-sh:before { + content: 'shell'; + } + + pre.src-sql:before { + content: 'SQL'; + } + + pre.src-sqlite:before { + content: 'SQLite'; + } + + /* additional languages in org.el's org-babel-load-languages alist */ + pre.src-forth:before { + content: 'Forth'; + } + + pre.src-io:before { + content: 'IO'; + } + + pre.src-J:before { + content: 'J'; + } + + pre.src-makefile:before { + content: 'Makefile'; + } + + pre.src-maxima:before { + content: 'Maxima'; + } + + pre.src-perl:before { + content: 'Perl'; + } + + pre.src-picolisp:before { + content: 'Pico Lisp'; + } + + pre.src-scala:before { + content: 'Scala'; + } + + pre.src-shell:before { + content: 'Shell Script'; + } + + pre.src-ebnf2ps:before { + content: 'ebfn2ps'; + } + + /* additional language identifiers per "defun org-babel-execute" + in ob-*.el */ + pre.src-cpp:before { + content: 'C++'; + } + + pre.src-abc:before { + content: 'ABC'; + } + + pre.src-coq:before { + content: 'Coq'; + } + + pre.src-groovy:before { + content: 'Groovy'; + } + + /* additional language identifiers from org-babel-shell-names in + ob-shell.el: ob-shell is the only babel language using a lambda to put + the execution function name together. */ + pre.src-bash:before { + content: 'bash'; + } + + pre.src-csh:before { + content: 'csh'; + } + + pre.src-ash:before { + content: 'ash'; + } + + pre.src-dash:before { + content: 'dash'; + } + + pre.src-ksh:before { + content: 'ksh'; + } + + pre.src-mksh:before { + content: 'mksh'; + } + + pre.src-posh:before { + content: 'posh'; + } + + /* Additional Emacs modes also supported by the LaTeX listings package */ + pre.src-ada:before { + content: 'Ada'; + } + + pre.src-asm:before { + content: 'Assembler'; + } + + pre.src-caml:before { + content: 'Caml'; + } + + pre.src-delphi:before { + content: 'Delphi'; + } + + pre.src-html:before { + content: 'HTML'; + } + + pre.src-idl:before { + content: 'IDL'; + } + + pre.src-mercury:before { + content: 'Mercury'; + } + + pre.src-metapost:before { + content: 'MetaPost'; + } + + pre.src-modula-2:before { + content: 'Modula-2'; + } + + pre.src-pascal:before { + content: 'Pascal'; + } + + pre.src-ps:before { + content: 'PostScript'; + } + + pre.src-prolog:before { + content: 'Prolog'; + } + + pre.src-simula:before { + content: 'Simula'; + } + + pre.src-tcl:before { + content: 'tcl'; + } + + pre.src-tex:before { + content: 'TeX'; + } + + pre.src-plain-tex:before { + content: 'Plain TeX'; + } + + pre.src-verilog:before { + content: 'Verilog'; + } + + pre.src-vhdl:before { + content: 'VHDL'; + } + + pre.src-xml:before { + content: 'XML'; + } + + pre.src-nxml:before { + content: 'XML'; + } + + /* add a generic configuration mode; LaTeX export needs an additional + (add-to-list 'org-latex-listings-langs '(conf " ")) in .emacs */ + pre.src-conf:before { + content: 'Configuration File'; + } + + table { + border-collapse: collapse; + } + + caption.t-above { + caption-side: top; + } + + caption.t-bottom { + caption-side: bottom; + } + + td, th { + vertical-align: top; + } + + th.org-right { + text-align: center; + } + + th.org-left { + text-align: center; + } + + th.org-center { + text-align: center; + } + + td.org-right { + text-align: right; + } + + td.org-left { + text-align: left; + } + + td.org-center { + text-align: center; + } + + dt { + font-weight: bold; + } + + .footpara { + display: inline; + } + + .footdef { + margin-bottom: 1em; + } + + .figure { + padding: 1em; + } + + .figure p { + text-align: center; + } + + .equation-container { + display: table; + text-align: center; + width: 100%; + } + + .equation { + vertical-align: middle; + } + + .equation-label { + display: table-cell; + text-align: right; + vertical-align: middle; + } + + .inlinetask { + padding: 10px; + border: 2px solid gray; + margin: 10px; + background: #ffffcc; + } + + #org-div-home-and-up { + text-align: right; + font-size: 70%; + white-space: nowrap; + } + + textarea { + overflow-x: auto; + } + + .linenr { + font-size: smaller + } + + .code-highlighted { + background-color: #ffff00; + } + + .org-info-js_info-navigation { + border-style: none; + } + + #org-info-js_console-label { + font-size: 10px; + font-weight: bold; + white-space: nowrap; + } + + .org-info-js_search-highlight { + background-color: #ffff00; + color: #000000; + font-weight: bold; + } + + .org-svg { + width: 90%; + } + + /*]]>*/ + --> + </style> + <script type="text/javascript"> + /* + @licstart The following is the entire license notice for the + JavaScript code in this tag. + + Copyright (C) 2012-2020 Free Software Foundation, Inc. + + The JavaScript code in this tag is free software: you can + redistribute it and/or modify it under the terms of the GNU + General Public License (GNU GPL) as published by the Free Software + Foundation, either version 3 of the License, or (at your option) + any later version. The code is distributed WITHOUT ANY WARRANTY; + without even the implied warranty of MERCHANTABILITY or FITNESS + FOR A PARTICULAR PURPOSE. See the GNU GPL for more details. + + As additional permission under GNU GPL version 3 section 7, you + may distribute non-source (e.g., minimized or compacted) forms of + that code without the copy of the GNU GPL normally required by + section 4, provided you include this license notice and a URL + through which recipients can access the Corresponding Source. + + + @licend The above is the entire license notice + for the JavaScript code in this tag. + */ + <!--/*--><![CDATA[/*><!--*/ + function CodeHighlightOn(elem, id) { + var target = document.getElementById(id); + if (null != target) { + elem.cacheClassElem = elem.className; + elem.cacheClassTarget = target.className; + target.className = "code-highlighted"; + elem.className = "code-highlighted"; + } + } + + function CodeHighlightOff(elem, id) { + var target = document.getElementById(id); + if (elem.cacheClassElem) + elem.className = elem.cacheClassElem; + if (elem.cacheClassTarget) + target.className = elem.cacheClassTarget; + } + + /*]]>*///--> + </script> </head> <body> <div id="content"> -<h1 class="title">About/FAQ</h1> -<div id="table-of-contents"> -<h2>Table of Contents</h2> -<div id="text-table-of-contents"> -<ul> -<li><a href="#org0db9061">1. What is the 'public sequence resource' about?</a></li> -<li><a href="#org983877d">2. Who created the public sequence resource?</a></li> -<li><a href="#org83093c3">3. How does the public sequence resource compare to other data resources?</a></li> -<li><a href="#org9b31fd4">4. Why should I upload my data here?</a></li> -<li><a href="#org4e92cb5">5. Why should I not upload by data here?</a></li> -<li><a href="#orgdfe72f6">6. How does the public sequence resource work?</a></li> -<li><a href="#orgd0c5abb">7. Who uses the public sequence resource?</a></li> -<li><a href="#org56f4a54">8. How can I contribute?</a></li> -<li><a href="#org2240ef7">9. Is this about open data?</a></li> -<li><a href="#orgbb655e0">10. Is this about free software?</a></li> -<li><a href="#org4e779f4">11. How do I upload raw data?</a></li> -<li><a href="#org83f6b7b">12. How do I change metadata?</a></li> -<li><a href="#org1bc6dab">13. How do I change the work flows?</a></li> -<li><a href="#org1140d62">14. How do I change the source code?</a></li> -<li><a href="#orge182714">15. Should I choose CC-BY or CC0?</a></li> -<li><a href="#orgf4a692b">16. How do I deal with private data and privacy?</a></li> -<li><a href="#org7757574">17. How do I communicate with you?</a></li> -<li><a href="#org194006f">18. Who are the sponsors?</a></li> -</ul> -</div> -</div> - -<div id="outline-container-org0db9061" class="outline-2"> -<h2 id="org0db9061"><span class="section-number-2">1</span> What is the 'public sequence resource' about?</h2> -<div class="outline-text-2" id="text-1"> -<p> -The <b>public sequence resource</b> aims to provide a generic and useful -resource for COVID-19 research. The focus is on providing the best -possible sequence data with associated metadata that can be used for -sequence comparison and protein prediction. -</p> -</div> -</div> - -<div id="outline-container-org983877d" class="outline-2"> -<h2 id="org983877d"><span class="section-number-2">2</span> Who created the public sequence resource?</h2> -<div class="outline-text-2" id="text-2"> -<p> -The <b>public sequence resource</b> is an initiative by <a href="https://github.com/arvados/bh20-seq-resource/graphs/contributors">bioinformatics</a> and -ontology experts who want to create something agile and useful for the -wider research community. The initiative started at the COVID-19 -biohackathon in April 2020 and is ongoing. The main project drivers -are Pjotr Prins (UTHSC), Peter Amstutz (Curii), Andrea Guarracino -(University of Rome Tor Vergata), Michael Crusoe (Common Workflow -Language), Thomas Liener (consultant, formerly EBI), Erik Garrison -(UCSC) and Jerven Bolleman (Swiss Institute of Bioinformatics). -</p> - -<p> -Notably, as this is a free software initiative, the project represents -major work by hundreds of software developers and ontology and data -wrangling experts. Thank you everyone! -</p> -</div> -</div> - -<div id="outline-container-org83093c3" class="outline-2"> -<h2 id="org83093c3"><span class="section-number-2">3</span> How does the public sequence resource compare to other data resources?</h2> -<div class="outline-text-2" id="text-3"> -<p> -The short version is that we use state-of-the-art practices in -bioinformatics using agile methods. Unlike the resources from large -institutes we can improve things on a dime and anyone can contribute -to building out this resource! Sequences from GenBank, EBI/ENA and -others are regularly added to PubSeq. We encourage people to everyone -to submit on PubSeq because of its superior live tooling and metadata -support (see the next question). -</p> - -<p> -Importantly: all data is published under either the <a href="https://creativecommons.org/licenses/by/4.0/">Creative Commons -4.0 attribution license</a> or the <a href="https://creativecommons.org/share-your-work/public-domain/cc0/">CC0 “No Rights Reserved” license</a> which -means it data can be published and workflows can run in public -environments allowing for improved access for research and -reproducible results. This contrasts with some other public resources, -such as GISAID. -</p> -</div> -</div> - -<div id="outline-container-org9b31fd4" class="outline-2"> -<h2 id="org9b31fd4"><span class="section-number-2">4</span> Why should I upload my data here?</h2> -<div class="outline-text-2" id="text-4"> -<ol class="org-ol"> -<li>We champion truly shareable data without licensing restrictions - with proper -attribution</li> -<li>We provide full metadata support using state-of-the-art ontology's</li> -<li>We provide a web-based sequence uploader and a command-line version -for bulk uploads</li> -<li>We provide a live SPARQL end-point for all metadata</li> -<li>We provide free data analysis and sequence comparison triggered on data upload</li> -<li>We do real work for you, with this <a href="https://workbench.lugli.arvadosapi.com/container_requests/lugli-xvhdp-bhhk4nxx1lch5od">link</a> you can see the last -run took 5.5 hours!</li> -<li>We provide free downloads of all computed output</li> -<li>There is no need to set up pipelines and/or compute clusters</li> -<li>All workflows get triggered on uploading a new sequence</li> -<li>When someone (you?) improves the software/workflows and everyone benefits</li> -<li>Your data gets automatically integrated with the Swiss Institure of -Bioinformatics COVID-19 knowledge base -<a href="https://covid-19-sparql.expasy.org/">https://covid-19-sparql.expasy.org/</a> (Elixir Switzerland)</li> -<li>Your data will be used to develop drug targets</li> -</ol> - -<p> -Finally, if you upload your data here we have workflows that output -formatted data suitable for <a href="http://covid19.genenetwork.org/blog?id=using-covid-19-pubseq-part6">uploading to EBI resources</a> (and soon -others). Uploading your data here get your data ready for upload to -multiple resources. -</p> -</div> -</div> - -<div id="outline-container-org4e92cb5" class="outline-2"> -<h2 id="org4e92cb5"><span class="section-number-2">5</span> Why should I not upload by data here?</h2> -<div class="outline-text-2" id="text-5"> -<p> -Funny question. There are only good reasons to upload your data here -and make it available to the widest audience possible. -</p> - -<p> -In fact, you can upload your data here as well as to other -resources. It is your data after all. No one can prevent you from -uploading your data to multiple resources. -</p> - -<p> -We recommend uploading to EBI and NCBI resources using our data -conversion tools. It means you only enter data once and make the -process smooth. You can also use our command line data uploader -for bulk uploads! -</p> -</div> -</div> - -<div id="outline-container-orgdfe72f6" class="outline-2"> -<h2 id="orgdfe72f6"><span class="section-number-2">6</span> How does the public sequence resource work?</h2> -<div class="outline-text-2" id="text-6"> -<p> -On uploading a sequence with metadata it will automatically be -processed and incorporated into the public pangenome with metadata -using workflows from the High Performance Open Biology Lab defined -<a href="https://github.com/hpobio-lab/viral-analysis/tree/master/cwl/pangenome-generate">here</a>. -</p> -</div> -</div> - -<div id="outline-container-orgd0c5abb" class="outline-2"> -<h2 id="orgd0c5abb"><span class="section-number-2">7</span> Who uses the public sequence resource?</h2> -<div class="outline-text-2" id="text-7"> -<p> -The Swiss Institute of Bioinformatics has included this data in -<a href="https://covid-19-sparql.expasy.org/">https://covid-19-sparql.expasy.org/</a> and made it part of <a href="https://www.uniprot.org/">Uniprot</a>. -</p> - -<p> -The Pantograph <a href="https://graph-genome.github.io/">viewer</a> uses PubSeq data for their visualisations. -</p> - -<p> -<a href="https://uthsc.edu">UTHSC</a> (USA), <a href="https://www.esr.cri.nz/">ESR</a> (New Zealand) and <a href="https://www.ornl.gov/news/ornl-fight-against-covid-19">ORNL</a> (USA) use COVID-19 PubSeq data -for monitoring, protein prediction and drug development. -</p> -</div> -</div> - -<div id="outline-container-org56f4a54" class="outline-2"> -<h2 id="org56f4a54"><span class="section-number-2">8</span> How can I contribute?</h2> -<div class="outline-text-2" id="text-8"> -<p> -You can contribute by submitting sequences, updating metadata, submit -issues on our issue tracker, and more importantly add functionality. -See 'How do I change the source code' below. Read through our online -documentation at <a href="http://covid19.genenetwork.org/blog">http://covid19.genenetwork.org/blog</a> as a starting -point. -</p> -</div> -</div> - -<div id="outline-container-org2240ef7" class="outline-2"> -<h2 id="org2240ef7"><span class="section-number-2">9</span> Is this about open data?</h2> -<div class="outline-text-2" id="text-9"> -<p> -All data is published under a <a href="https://creativecommons.org/licenses/by/4.0/">Creative Commons 4.0 attribution license</a> -(CC-BY-4.0). You can download the raw and published (GFA/RDF/FASTA) -data and store it for further processing. -</p> -</div> -</div> - -<div id="outline-container-orgbb655e0" class="outline-2"> -<h2 id="orgbb655e0"><span class="section-number-2">10</span> Is this about free software?</h2> -<div class="outline-text-2" id="text-10"> -<p> -Absolutely. Free software allows for fully reproducible pipelines. You -can take our workflows and data and run it elsewhere! -</p> -</div> -</div> - -<div id="outline-container-org4e779f4" class="outline-2"> -<h2 id="org4e779f4"><span class="section-number-2">11</span> How do I upload raw data?</h2> -<div class="outline-text-2" id="text-11"> -<p> -We are preparing raw sequence data pipelines (fastq and BAM). The -reason is that we want the best data possible for downstream analysis -(including protein prediction and test development). The current -approach where people publish final sequences of SARS-CoV-2 is lacking -because it hides how this sequence was created. For reasons of -reproducible and improved results we want/need to work with the raw -sequence reads (both short reads and long reads) and take alternative -assembly variations into consideration. This is all work in progress. -</p> -</div> -</div> - -<div id="outline-container-org83f6b7b" class="outline-2"> -<h2 id="org83f6b7b"><span class="section-number-2">12</span> How do I change metadata?</h2> -<div class="outline-text-2" id="text-12"> -<p> -See the <a href="http://covid19.genenetwork.org/blog">http://covid19.genenetwork.org/blog</a>! -</p> -</div> -</div> - -<div id="outline-container-org1bc6dab" class="outline-2"> -<h2 id="org1bc6dab"><span class="section-number-2">13</span> How do I change the work flows?</h2> -<div class="outline-text-2" id="text-13"> -<p> -Workflows are on <a href="https://github.com/arvados/bh20-seq-resource/tree/master/workflows">github</a> and can be modified. See also the BLOG -<a href="http://covid19.genenetwork.org/blog">http://covid19.genenetwork.org/blog</a> on workflows. -</p> -</div> -</div> - -<div id="outline-container-org1140d62" class="outline-2"> -<h2 id="org1140d62"><span class="section-number-2">14</span> How do I change the source code?</h2> -<div class="outline-text-2" id="text-14"> -<p> -Go to our <a href="https://github.com/arvados/bh20-seq-resource">source code repositories</a>, fork/clone the repository, change -something and submit a <a href="https://github.com/arvados/bh20-seq-resource/pulls">pull request</a> (PR). That easy! Check out how -many PRs we already merged. -</p> -</div> -</div> - -<div id="outline-container-orge182714" class="outline-2"> -<h2 id="orge182714"><span class="section-number-2">15</span> Should I choose CC-BY or CC0?</h2> -<div class="outline-text-2" id="text-15"> -<p> -Restrictive data licenses are hampering data sharing and reproducible -research. CC0 is the preferred license because it gives researchers -the most freedom. Since we provide metadata there is no reason for -others not to honour your work. We also provide CC-BY as an option -because we know people like the attribution clause. -</p> - -<p> -In all honesty: we prefer both data and software to be free. -</p> -</div> -</div> - -<div id="outline-container-orgf4a692b" class="outline-2"> -<h2 id="orgf4a692b"><span class="section-number-2">16</span> How do I deal with private data and privacy?</h2> -<div class="outline-text-2" id="text-16"> -<p> -A public sequence resource is about public data. Metadata can refer to -private data. You can use your own (anonymous) identifiers. We also -plan to combine identifiers with clinical data stored securely at -<a href="https://redcap-covid19.elixir-luxembourg.org/redcap/">REDCap</a>. See the relevant <a href="https://github.com/arvados/bh20-seq-resource/issues/21">tracker</a> for more information and contributing. -</p> -</div> -</div> - -<div id="outline-container-org7757574" class="outline-2"> -<h2 id="org7757574"><span class="section-number-2">17</span> How do I communicate with you?</h2> -<div class="outline-text-2" id="text-17"> -<p> -We use a <a href="https://gitter.im/arvados/pubseq?utm_source=share-link&utm_medium=link&utm_campaign=share-link">gitter channel</a> you can join. -</p> -</div> -</div> - -<div id="outline-container-org194006f" class="outline-2"> -<h2 id="org194006f"><span class="section-number-2">18</span> Who are the sponsors?</h2> -<div class="outline-text-2" id="text-18"> -<p> -The main sponsors are listed in the footer. In addition to the time -generously donated by many contributors we also acknowledge Amazon AWS -for donating COVID-19 related compute time. -</p> -</div> -</div> + <h1 class="title">About/FAQ</h1> + <div id="table-of-contents"> + <h2>Table of Contents</h2> + <div id="text-table-of-contents"> + <ul> + <li><a href="#org0db9061">1. What is the 'public sequence resource' about?</a></li> + <li><a href="#org983877d">2. Who created the public sequence resource?</a></li> + <li><a href="#org83093c3">3. How does the public sequence resource compare to other data resources?</a> + </li> + <li><a href="#org9b31fd4">4. Why should I upload my data here?</a></li> + <li><a href="#org4e92cb5">5. Why should I not upload by data here?</a></li> + <li><a href="#orgdfe72f6">6. How does the public sequence resource work?</a></li> + <li><a href="#orgd0c5abb">7. Who uses the public sequence resource?</a></li> + <li><a href="#org56f4a54">8. How can I contribute?</a></li> + <li><a href="#org2240ef7">9. Is this about open data?</a></li> + <li><a href="#orgbb655e0">10. Is this about free software?</a></li> + <li><a href="#org4e779f4">11. How do I upload raw data?</a></li> + <li><a href="#org83f6b7b">12. How do I change metadata?</a></li> + <li><a href="#org1bc6dab">13. How do I change the work flows?</a></li> + <li><a href="#org1140d62">14. How do I change the source code?</a></li> + <li><a href="#orge182714">15. Should I choose CC-BY or CC0?</a></li> + <li><a href="#orgf4a692b">16. How do I deal with private data and privacy?</a></li> + <li><a href="#org7757574">17. How do I communicate with you?</a></li> + <li><a href="#org194006f">18. Who are the sponsors?</a></li> + </ul> + </div> + </div> + + <div id="outline-container-org0db9061" class="outline-2"> + <h2 id="org0db9061"><span class="section-number-2">1</span> What is the 'public sequence resource' about?</h2> + <div class="outline-text-2" id="text-1"> + <p> + The <b>public sequence resource</b> aims to provide a generic and useful + resource for COVID-19 research. The focus is on providing the best + possible sequence data with associated metadata that can be used for + sequence comparison and protein prediction. + </p> + <p> + We were at the <strong>Bioinformatics Community Conference 2020</strong>! Have a look at the + <a href="https://bcc2020.sched.com/event/coLw">video talk</a></li> + (<a href="https://drive.google.com/file/d/1skXHwVKM_gl73-_4giYIOQ1IlC5X5uBo/view?usp=sharing">alternative link</a>) + and the <a href="https://drive.google.com/file/d/1vyEgfvSqhM9yIwWZ6Iys-QxhxtVxPSdp/view?usp=sharing">poster</a>. + </p> + </div> + </div> + + <div id="outline-container-org983877d" class="outline-2"> + <h2 id="org983877d"><span class="section-number-2">2</span> Who created the public sequence resource?</h2> + <div class="outline-text-2" id="text-2"> + <p> + The <b>public sequence resource</b> is an initiative by <a + href="https://github.com/arvados/bh20-seq-resource/graphs/contributors">bioinformatics</a> and + ontology experts who want to create something agile and useful for the + wider research community. The initiative started at the COVID-19 + biohackathon in April 2020 and is ongoing. The main project drivers + are Pjotr Prins (UTHSC), Peter Amstutz (Curii), Andrea Guarracino + (University of Rome Tor Vergata), Michael Crusoe (Common Workflow + Language), Thomas Liener (consultant, formerly EBI), Erik Garrison + (UCSC) and Jerven Bolleman (Swiss Institute of Bioinformatics). + </p> + + <p> + Notably, as this is a free software initiative, the project represents + major work by hundreds of software developers and ontology and data + wrangling experts. Thank you everyone! + </p> + </div> + </div> + + <div id="outline-container-org83093c3" class="outline-2"> + <h2 id="org83093c3"><span class="section-number-2">3</span> How does the public sequence resource compare to + other data resources?</h2> + <div class="outline-text-2" id="text-3"> + <p> + The short version is that we use state-of-the-art practices in + bioinformatics using agile methods. Unlike the resources from large + institutes we can improve things on a dime and anyone can contribute + to building out this resource! Sequences from GenBank, EBI/ENA and + others are regularly added to PubSeq. We encourage people to everyone + to submit on PubSeq because of its superior live tooling and metadata + support (see the next question). + </p> + + <p> + Importantly: all data is published under either the <a + href="https://creativecommons.org/licenses/by/4.0/">Creative Commons + 4.0 attribution license</a> or the <a + href="https://creativecommons.org/share-your-work/public-domain/cc0/">CC0 “No Rights Reserved” + license</a> which + means it data can be published and workflows can run in public + environments allowing for improved access for research and + reproducible results. This contrasts with some other public resources, + such as GISAID. + </p> + </div> + </div> + + <div id="outline-container-org9b31fd4" class="outline-2"> + <h2 id="org9b31fd4"><span class="section-number-2">4</span> Why should I upload my data here?</h2> + <div class="outline-text-2" id="text-4"> + <ol class="org-ol"> + <li>We champion truly shareable data without licensing restrictions - with proper + attribution + </li> + <li>We provide full metadata support using state-of-the-art ontology's</li> + <li>We provide a web-based sequence uploader and a command-line version + for bulk uploads + </li> + <li>We provide a live SPARQL end-point for all metadata</li> + <li>We provide free data analysis and sequence comparison triggered on data upload</li> + <li>We do real work for you, with this <a + href="https://workbench.lugli.arvadosapi.com/container_requests/lugli-xvhdp-bhhk4nxx1lch5od">link</a> + you can see the last + run took 5.5 hours! + </li> + <li>We provide free downloads of all computed output</li> + <li>There is no need to set up pipelines and/or compute clusters</li> + <li>All workflows get triggered on uploading a new sequence</li> + <li>When someone (you?) improves the software/workflows and everyone benefits</li> + <li>Your data gets automatically integrated with the Swiss Institure of + Bioinformatics COVID-19 knowledge base + <a href="https://covid-19-sparql.expasy.org/">https://covid-19-sparql.expasy.org/</a> (Elixir + Switzerland) + </li> + <li>Your data will be used to develop drug targets</li> + </ol> + + <p> + Finally, if you upload your data here we have workflows that output + formatted data suitable for <a + href="http://covid19.genenetwork.org/blog?id=using-covid-19-pubseq-part6">uploading to EBI + resources</a> (and soon + others). Uploading your data here get your data ready for upload to + multiple resources. + </p> + </div> + </div> + + <div id="outline-container-org4e92cb5" class="outline-2"> + <h2 id="org4e92cb5"><span class="section-number-2">5</span> Why should I not upload by data here?</h2> + <div class="outline-text-2" id="text-5"> + <p> + Funny question. There are only good reasons to upload your data here + and make it available to the widest audience possible. + </p> + + <p> + In fact, you can upload your data here as well as to other + resources. It is your data after all. No one can prevent you from + uploading your data to multiple resources. + </p> + + <p> + We recommend uploading to EBI and NCBI resources using our data + conversion tools. It means you only enter data once and make the + process smooth. You can also use our command line data uploader + for bulk uploads! + </p> + </div> + </div> + + <div id="outline-container-orgdfe72f6" class="outline-2"> + <h2 id="orgdfe72f6"><span class="section-number-2">6</span> How does the public sequence resource work?</h2> + <div class="outline-text-2" id="text-6"> + <p> + On uploading a sequence with metadata it will automatically be + processed and incorporated into the public pangenome with metadata + using workflows from the High Performance Open Biology Lab defined + <a href="https://github.com/hpobio-lab/viral-analysis/tree/master/cwl/pangenome-generate">here</a>. + </p> + </div> + </div> + + <div id="outline-container-orgd0c5abb" class="outline-2"> + <h2 id="orgd0c5abb"><span class="section-number-2">7</span> Who uses the public sequence resource?</h2> + <div class="outline-text-2" id="text-7"> + <p> + The Swiss Institute of Bioinformatics has included this data in + <a href="https://covid-19-sparql.expasy.org/">https://covid-19-sparql.expasy.org/</a> and made it part + of <a href="https://www.uniprot.org/">Uniprot</a>. + </p> + + <p> + The Pantograph <a href="https://graph-genome.github.io/">viewer</a> uses PubSeq data for their + visualisations. + </p> + + <p> + <a href="https://uthsc.edu">UTHSC</a> (USA), <a href="https://www.esr.cri.nz/">ESR</a> (New Zealand) and + <a href="https://www.ornl.gov/news/ornl-fight-against-covid-19">ORNL</a> (USA) use COVID-19 PubSeq data + for monitoring, protein prediction and drug development. + </p> + </div> + </div> + + <div id="outline-container-org56f4a54" class="outline-2"> + <h2 id="org56f4a54"><span class="section-number-2">8</span> How can I contribute?</h2> + <div class="outline-text-2" id="text-8"> + <p> + You can contribute by submitting sequences, updating metadata, submit + issues on our issue tracker, and more importantly add functionality. + See 'How do I change the source code' below. Read through our online + documentation at <a href="http://covid19.genenetwork.org/blog">http://covid19.genenetwork.org/blog</a> + as a starting + point. + </p> + </div> + </div> + + <div id="outline-container-org2240ef7" class="outline-2"> + <h2 id="org2240ef7"><span class="section-number-2">9</span> Is this about open data?</h2> + <div class="outline-text-2" id="text-9"> + <p> + All data is published under a <a href="https://creativecommons.org/licenses/by/4.0/">Creative Commons + 4.0 attribution license</a> + (CC-BY-4.0). You can download the raw and published (GFA/RDF/FASTA) + data and store it for further processing. + </p> + </div> + </div> + + <div id="outline-container-orgbb655e0" class="outline-2"> + <h2 id="orgbb655e0"><span class="section-number-2">10</span> Is this about free software?</h2> + <div class="outline-text-2" id="text-10"> + <p> + Absolutely. Free software allows for fully reproducible pipelines. You + can take our workflows and data and run it elsewhere! + </p> + </div> + </div> + + <div id="outline-container-org4e779f4" class="outline-2"> + <h2 id="org4e779f4"><span class="section-number-2">11</span> How do I upload raw data?</h2> + <div class="outline-text-2" id="text-11"> + <p> + We are preparing raw sequence data pipelines (fastq and BAM). The + reason is that we want the best data possible for downstream analysis + (including protein prediction and test development). The current + approach where people publish final sequences of SARS-CoV-2 is lacking + because it hides how this sequence was created. For reasons of + reproducible and improved results we want/need to work with the raw + sequence reads (both short reads and long reads) and take alternative + assembly variations into consideration. This is all work in progress. + </p> + </div> + </div> + + <div id="outline-container-org83f6b7b" class="outline-2"> + <h2 id="org83f6b7b"><span class="section-number-2">12</span> How do I change metadata?</h2> + <div class="outline-text-2" id="text-12"> + <p> + See the <a href="http://covid19.genenetwork.org/blog">http://covid19.genenetwork.org/blog</a>! + </p> + </div> + </div> + + <div id="outline-container-org1bc6dab" class="outline-2"> + <h2 id="org1bc6dab"><span class="section-number-2">13</span> How do I change the work flows?</h2> + <div class="outline-text-2" id="text-13"> + <p> + Workflows are on <a href="https://github.com/arvados/bh20-seq-resource/tree/master/workflows">github</a> + and can be modified. See also the BLOG + <a href="http://covid19.genenetwork.org/blog">http://covid19.genenetwork.org/blog</a> on workflows. + </p> + </div> + </div> + + <div id="outline-container-org1140d62" class="outline-2"> + <h2 id="org1140d62"><span class="section-number-2">14</span> How do I change the source code?</h2> + <div class="outline-text-2" id="text-14"> + <p> + Go to our <a href="https://github.com/arvados/bh20-seq-resource">source code repositories</a>, + fork/clone the repository, change + something and submit a <a href="https://github.com/arvados/bh20-seq-resource/pulls">pull request</a> + (PR). That easy! Check out how + many PRs we already merged. + </p> + </div> + </div> + + <div id="outline-container-orge182714" class="outline-2"> + <h2 id="orge182714"><span class="section-number-2">15</span> Should I choose CC-BY or CC0?</h2> + <div class="outline-text-2" id="text-15"> + <p> + Restrictive data licenses are hampering data sharing and reproducible + research. CC0 is the preferred license because it gives researchers + the most freedom. Since we provide metadata there is no reason for + others not to honour your work. We also provide CC-BY as an option + because we know people like the attribution clause. + </p> + + <p> + In all honesty: we prefer both data and software to be free. + </p> + </div> + </div> + + <div id="outline-container-orgf4a692b" class="outline-2"> + <h2 id="orgf4a692b"><span class="section-number-2">16</span> How do I deal with private data and privacy?</h2> + <div class="outline-text-2" id="text-16"> + <p> + A public sequence resource is about public data. Metadata can refer to + private data. You can use your own (anonymous) identifiers. We also + plan to combine identifiers with clinical data stored securely at + <a href="https://redcap-covid19.elixir-luxembourg.org/redcap/">REDCap</a>. See the relevant <a + href="https://github.com/arvados/bh20-seq-resource/issues/21">tracker</a> for more information and + contributing. + </p> + </div> + </div> + + <div id="outline-container-org7757574" class="outline-2"> + <h2 id="org7757574"><span class="section-number-2">17</span> How do I communicate with you?</h2> + <div class="outline-text-2" id="text-17"> + <p> + We use a <a + href="https://gitter.im/arvados/pubseq?utm_source=share-link&utm_medium=link&utm_campaign=share-link">gitter + channel</a> you can join. + </p> + </div> + </div> + + <div id="outline-container-org194006f" class="outline-2"> + <h2 id="org194006f"><span class="section-number-2">18</span> Who are the sponsors?</h2> + <div class="outline-text-2" id="text-18"> + <p> + The main sponsors are listed in the footer. In addition to the time + generously donated by many contributors we also acknowledge Amazon AWS + for donating COVID-19 related compute time. + </p> + </div> + </div> </div> <div id="postamble" class="status"> -<hr><small>Created by <a href="http://thebird.nl/">Pjotr Prins</a> (pjotr.public768 at thebird 'dot' nl) using Emacs org-mode and a healthy dose of Lisp!<br />Modified 2020-07-18 Sat 03:27</small>. + <hr> + <small>Created by <a href="http://thebird.nl/">Pjotr Prins</a> (pjotr.public768 at thebird 'dot' nl) using Emacs + org-mode and a healthy dose of Lisp!<br/>Modified 2020-07-18 Sat 03:27</small>. </div> </body> </html> |