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author | Pjotr Prins | 2020-08-22 14:07:53 +0100 |
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committer | Pjotr Prins | 2020-08-22 14:07:53 +0100 |
commit | 246c516e4a8c98394c695dcb446995319d557e01 (patch) | |
tree | 08c81638a12fdb94e36285267e77755a2c25cdd7 /doc/blog | |
parent | f776816ee2b1af7ccc84afb494f68a81a51f5a76 (diff) | |
download | bh20-seq-resource-246c516e4a8c98394c695dcb446995319d557e01.tar.gz bh20-seq-resource-246c516e4a8c98394c695dcb446995319d557e01.tar.lz bh20-seq-resource-246c516e4a8c98394c695dcb446995319d557e01.zip |
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-rw-r--r-- | doc/blog/using-covid-19-pubseq-part3.org | 1 | ||||
-rw-r--r-- | doc/blog/using-covid-19-pubseq-part5.html | 130 | ||||
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4 files changed, 180 insertions, 106 deletions
diff --git a/doc/blog/using-covid-19-pubseq-part3.html b/doc/blog/using-covid-19-pubseq-part3.html index df4a286..80304c3 100644 --- a/doc/blog/using-covid-19-pubseq-part3.html +++ b/doc/blog/using-covid-19-pubseq-part3.html @@ -3,7 +3,7 @@ "http://www.w3.org/TR/xhtml1/DTD/xhtml1-strict.dtd"> <html xmlns="http://www.w3.org/1999/xhtml" lang="en" xml:lang="en"> <head> -<!-- 2020-05-30 Sat 18:12 --> +<!-- 2020-08-22 Sat 07:43 --> <meta http-equiv="Content-Type" content="text/html;charset=utf-8" /> <meta name="viewport" content="width=device-width, initial-scale=1" /> <title>COVID-19 PubSeq Uploading Data (part 3)</title> @@ -248,40 +248,40 @@ for the JavaScript code in this tag. <h2>Table of Contents</h2> <div id="text-table-of-contents"> <ul> -<li><a href="#org193669a">1. Uploading Data</a></li> -<li><a href="#orgc6b3a47">2. Step 1: Upload sequence</a></li> -<li><a href="#org9c08714">3. Step 2: Add metadata</a> +<li><a href="#org4419df1">1. Uploading Data</a></li> +<li><a href="#org53a95df">2. Step 1: Upload sequence</a></li> +<li><a href="#orga007292">3. Step 2: Add metadata</a> <ul> -<li><a href="#org4c2e907">3.1. Obligatory fields</a> +<li><a href="#org645875f">3.1. Obligatory fields</a> <ul> -<li><a href="#orgdddcb2e">3.1.1. Sample ID (sample<sub>id</sub>)</a></li> -<li><a href="#orge9c2e76">3.1.2. Collection date</a></li> -<li><a href="#org62c55ce">3.1.3. Collection location</a></li> -<li><a href="#org460b377">3.1.4. Sequencing technology</a></li> -<li><a href="#org77b1e14">3.1.5. Authors</a></li> +<li><a href="#org6e395e3">3.1.1. Sample ID (sample<sub>id</sub>)</a></li> +<li><a href="#org350066d">3.1.2. Collection date</a></li> +<li><a href="#org336b4af">3.1.3. Collection location</a></li> +<li><a href="#org66f0f8e">3.1.4. Sequencing technology</a></li> +<li><a href="#orgb370da6">3.1.5. Authors</a></li> </ul> </li> -<li><a href="#org3cb346f">3.2. Optional fields</a> +<li><a href="#orgaafe83d">3.2. Optional fields</a> <ul> -<li><a href="#orgb0cffbb">3.2.1. Host information</a></li> -<li><a href="#orgd2a43a6">3.2.2. Collecting institution</a></li> -<li><a href="#org8d5bcf7">3.2.3. Specimen source</a></li> -<li><a href="#org86b21b2">3.2.4. Source database accession</a></li> -<li><a href="#org771ea66">3.2.5. Strain name</a></li> +<li><a href="#org9708c5c">3.2.1. Host information</a></li> +<li><a href="#org5c6c880">3.2.2. Collecting institution</a></li> +<li><a href="#org1754a88">3.2.3. Specimen source</a></li> +<li><a href="#org00806b9">3.2.4. Source database accession</a></li> +<li><a href="#orga69b584">3.2.5. Strain name</a></li> </ul> </li> </ul> </li> -<li><a href="#org7d281f5">4. Step 3: Submit to COVID-19 PubSeq</a> +<li><a href="#org34a76cf">4. Step 3: Submit to COVID-19 PubSeq</a> <ul> -<li><a href="#orgdf0f02d">4.1. Trouble shooting</a></li> +<li><a href="#orge4dfc55">4.1. Trouble shooting</a></li> </ul> </li> -<li><a href="#org29f8a92">5. Step 4: Check output</a></li> -<li><a href="#orgf493854">6. Bulk sequence uploader</a> +<li><a href="#org0bbb27f">5. Step 4: Check output</a></li> +<li><a href="#orged64f4e">6. Bulk sequence uploader</a> <ul> -<li><a href="#org37fadbc">6.1. Run the uploader (CLI)</a></li> -<li><a href="#org39adf09">6.2. Example: uploading bulk GenBank sequences</a></li> +<li><a href="#orge1c3625">6.1. Run the uploader (CLI)</a></li> +<li><a href="#org07f04d9">6.2. Example: uploading bulk GenBank sequences</a></li> </ul> </li> </ul> @@ -290,8 +290,8 @@ for the JavaScript code in this tag. -<div id="outline-container-org193669a" class="outline-2"> -<h2 id="org193669a"><span class="section-number-2">1</span> Uploading Data</h2> +<div id="outline-container-org4419df1" class="outline-2"> +<h2 id="org4419df1"><span class="section-number-2">1</span> Uploading Data</h2> <div class="outline-text-2" id="text-1"> <p> The COVID-19 PubSeq allows you to upload your SARS-Cov-2 strains to a @@ -301,8 +301,8 @@ gets triggered on upload. Read the <a href="./about">ABOUT</a> page for more inf </div> </div> -<div id="outline-container-orgc6b3a47" class="outline-2"> -<h2 id="orgc6b3a47"><span class="section-number-2">2</span> Step 1: Upload sequence</h2> +<div id="outline-container-org53a95df" class="outline-2"> +<h2 id="org53a95df"><span class="section-number-2">2</span> Step 1: Upload sequence</h2> <div class="outline-text-2" id="text-2"> <p> To upload a sequence in the <a href="http://covid19.genenetwork.org/">web upload page</a> hit the browse button and @@ -330,8 +330,8 @@ an improved pangenome. </div> </div> -<div id="outline-container-org9c08714" class="outline-2"> -<h2 id="org9c08714"><span class="section-number-2">3</span> Step 2: Add metadata</h2> +<div id="outline-container-orga007292" class="outline-2"> +<h2 id="orga007292"><span class="section-number-2">3</span> Step 2: Add metadata</h2> <div class="outline-text-2" id="text-3"> <p> The <a href="./">web upload page</a> contains fields for adding metadata. Metadata is @@ -357,12 +357,12 @@ the web form. Here we add some extra information. </p> </div> -<div id="outline-container-org4c2e907" class="outline-3"> -<h3 id="org4c2e907"><span class="section-number-3">3.1</span> Obligatory fields</h3> +<div id="outline-container-org645875f" class="outline-3"> +<h3 id="org645875f"><span class="section-number-3">3.1</span> Obligatory fields</h3> <div class="outline-text-3" id="text-3-1"> </div> -<div id="outline-container-orgdddcb2e" class="outline-4"> -<h4 id="orgdddcb2e"><span class="section-number-4">3.1.1</span> Sample ID (sample<sub>id</sub>)</h4> +<div id="outline-container-org6e395e3" class="outline-4"> +<h4 id="org6e395e3"><span class="section-number-4">3.1.1</span> Sample ID (sample<sub>id</sub>)</h4> <div class="outline-text-4" id="text-3-1-1"> <p> This is a string field that defines a unique sample identifier by the @@ -380,8 +380,8 @@ Here we add the GenBank ID MT536190.1. </div> </div> -<div id="outline-container-orge9c2e76" class="outline-4"> -<h4 id="orge9c2e76"><span class="section-number-4">3.1.2</span> Collection date</h4> +<div id="outline-container-org350066d" class="outline-4"> +<h4 id="org350066d"><span class="section-number-4">3.1.2</span> Collection date</h4> <div class="outline-text-4" id="text-3-1-2"> <p> Estimated collection date. The GenBank page says April 6, 2020. @@ -389,8 +389,8 @@ Estimated collection date. The GenBank page says April 6, 2020. </div> </div> -<div id="outline-container-org62c55ce" class="outline-4"> -<h4 id="org62c55ce"><span class="section-number-4">3.1.3</span> Collection location</h4> +<div id="outline-container-org336b4af" class="outline-4"> +<h4 id="org336b4af"><span class="section-number-4">3.1.3</span> Collection location</h4> <div class="outline-text-4" id="text-3-1-3"> <p> A search on wikidata says Los Angeles is @@ -399,8 +399,8 @@ A search on wikidata says Los Angeles is </div> </div> -<div id="outline-container-org460b377" class="outline-4"> -<h4 id="org460b377"><span class="section-number-4">3.1.4</span> Sequencing technology</h4> +<div id="outline-container-org66f0f8e" class="outline-4"> +<h4 id="org66f0f8e"><span class="section-number-4">3.1.4</span> Sequencing technology</h4> <div class="outline-text-4" id="text-3-1-4"> <p> GenBank entry says Illumina, so we can fill that in @@ -408,8 +408,8 @@ GenBank entry says Illumina, so we can fill that in </div> </div> -<div id="outline-container-org77b1e14" class="outline-4"> -<h4 id="org77b1e14"><span class="section-number-4">3.1.5</span> Authors</h4> +<div id="outline-container-orgb370da6" class="outline-4"> +<h4 id="orgb370da6"><span class="section-number-4">3.1.5</span> Authors</h4> <div class="outline-text-4" id="text-3-1-5"> <p> GenBank entry says 'Lamers,S., Nolan,D.J., Rose,R., Cross,S., Moraga @@ -420,16 +420,16 @@ Freehan,A. and Garcia-Diaz,J.', so we can fill that in. </div> </div> -<div id="outline-container-org3cb346f" class="outline-3"> -<h3 id="org3cb346f"><span class="section-number-3">3.2</span> Optional fields</h3> +<div id="outline-container-orgaafe83d" class="outline-3"> +<h3 id="orgaafe83d"><span class="section-number-3">3.2</span> Optional fields</h3> <div class="outline-text-3" id="text-3-2"> <p> All other fields are optional. But let's see what we can add. </p> </div> -<div id="outline-container-orgb0cffbb" class="outline-4"> -<h4 id="orgb0cffbb"><span class="section-number-4">3.2.1</span> Host information</h4> +<div id="outline-container-org9708c5c" class="outline-4"> +<h4 id="org9708c5c"><span class="section-number-4">3.2.1</span> Host information</h4> <div class="outline-text-4" id="text-3-2-1"> <p> Sadly, not much is known about the host from GenBank. A little @@ -443,8 +443,8 @@ did to the person and what the person was like (say age group). </div> </div> -<div id="outline-container-orgd2a43a6" class="outline-4"> -<h4 id="orgd2a43a6"><span class="section-number-4">3.2.2</span> Collecting institution</h4> +<div id="outline-container-org5c6c880" class="outline-4"> +<h4 id="org5c6c880"><span class="section-number-4">3.2.2</span> Collecting institution</h4> <div class="outline-text-4" id="text-3-2-2"> <p> We can fill that in. @@ -452,8 +452,8 @@ We can fill that in. </div> </div> -<div id="outline-container-org8d5bcf7" class="outline-4"> -<h4 id="org8d5bcf7"><span class="section-number-4">3.2.3</span> Specimen source</h4> +<div id="outline-container-org1754a88" class="outline-4"> +<h4 id="org1754a88"><span class="section-number-4">3.2.3</span> Specimen source</h4> <div class="outline-text-4" id="text-3-2-3"> <p> We have that: nasopharyngeal swab @@ -461,8 +461,8 @@ We have that: nasopharyngeal swab </div> </div> -<div id="outline-container-org86b21b2" class="outline-4"> -<h4 id="org86b21b2"><span class="section-number-4">3.2.4</span> Source database accession</h4> +<div id="outline-container-org00806b9" class="outline-4"> +<h4 id="org00806b9"><span class="section-number-4">3.2.4</span> Source database accession</h4> <div class="outline-text-4" id="text-3-2-4"> <p> Genbank which is <a href="http://identifiers.org/insdc/MT536190.1#sequence">http://identifiers.org/insdc/MT536190.1#sequence</a>. @@ -471,8 +471,8 @@ Note we plug in our own identifier MT536190.1. </div> </div> -<div id="outline-container-org771ea66" class="outline-4"> -<h4 id="org771ea66"><span class="section-number-4">3.2.5</span> Strain name</h4> +<div id="outline-container-orga69b584" class="outline-4"> +<h4 id="orga69b584"><span class="section-number-4">3.2.5</span> Strain name</h4> <div class="outline-text-4" id="text-3-2-5"> <p> SARS-CoV-2/human/USA/LA-BIE-070/2020 @@ -482,8 +482,8 @@ SARS-CoV-2/human/USA/LA-BIE-070/2020 </div> </div> -<div id="outline-container-org7d281f5" class="outline-2"> -<h2 id="org7d281f5"><span class="section-number-2">4</span> Step 3: Submit to COVID-19 PubSeq</h2> +<div id="outline-container-org34a76cf" class="outline-2"> +<h2 id="org34a76cf"><span class="section-number-2">4</span> Step 3: Submit to COVID-19 PubSeq</h2> <div class="outline-text-2" id="text-4"> <p> Once you have the sequence and the metadata together, hit @@ -493,8 +493,8 @@ submitted and the workflows should kick in! </div> -<div id="outline-container-orgdf0f02d" class="outline-3"> -<h3 id="orgdf0f02d"><span class="section-number-3">4.1</span> Trouble shooting</h3> +<div id="outline-container-orge4dfc55" class="outline-3"> +<h3 id="orge4dfc55"><span class="section-number-3">4.1</span> Trouble shooting</h3> <div class="outline-text-3" id="text-4-1"> <p> We got an error saying: {"stem": "<a href="http://www.wikidata.org/entity/">http://www.wikidata.org/entity/</a>",… @@ -508,9 +508,8 @@ submit button. </div> </div> - -<div id="outline-container-org29f8a92" class="outline-2"> -<h2 id="org29f8a92"><span class="section-number-2">5</span> Step 4: Check output</h2> +<div id="outline-container-org0bbb27f" class="outline-2"> +<h2 id="org0bbb27f"><span class="section-number-2">5</span> Step 4: Check output</h2> <div class="outline-text-2" id="text-5"> <p> The current pipeline takes 5.5 hours to complete! Once it completes @@ -521,8 +520,8 @@ in. </div> </div> -<div id="outline-container-orgf493854" class="outline-2"> -<h2 id="orgf493854"><span class="section-number-2">6</span> Bulk sequence uploader</h2> +<div id="outline-container-orged64f4e" class="outline-2"> +<h2 id="orged64f4e"><span class="section-number-2">6</span> Bulk sequence uploader</h2> <div class="outline-text-2" id="text-6"> <p> Above steps require a manual upload of one sequence with metadata. @@ -544,8 +543,8 @@ host: host_age: <span style="color: #8bc34a;">20</span> host_age_unit: http://purl.obolibrary.org/obo/UO_<span style="color: #8bc34a;">0000036</span> host_health_status: http://purl.obolibrary.org/obo/NCIT_C<span style="color: #8bc34a;">25269</span> - host_treatment: Process in which the act is intended to modify or alter host status <span style="color: #e91e63;">(</span>Compounds<span style="color: #e91e63;">)</span> - host_vaccination: <span style="color: #e91e63;">[</span>vaccines<span style="color: #8bc34a;">1</span>,vaccine<span style="color: #8bc34a;">2</span><span style="color: #e91e63;">]</span> + host_treatment: Process in which the act is intended to modify or alter host status (Compounds) + host_vaccination: [vaccines<span style="color: #8bc34a;">1</span>,vaccine<span style="color: #8bc34a;">2</span>] ethnicity: http://purl.obolibrary.org/obo/HANCESTRO_<span style="color: #8bc34a;">0010</span> additional_host_information: Optional free text field for additional information @@ -553,11 +552,11 @@ sample: sample_id: Id of the sample as defined by the submitter collector_name: Name of the person that took the sample collecting_institution: Institute that was responsible of sampling - specimen_source: <span style="color: #e91e63;">[</span>http://purl.obolibrary.org/obo/NCIT_C<span style="color: #8bc34a;">155831</span>,http://purl.obolibrary.org/obo/NCIT_C<span style="color: #8bc34a;">155835</span>] + specimen_source: [http://purl.obolibrary.org/obo/NCIT_C<span style="color: #8bc34a;">155831</span>,http://purl.obolibrary.org/obo/NCIT_C<span style="color: #8bc34a;">155835</span>] collection_date: <span style="color: #9ccc65;">"2020-01-01"</span> collection_location: http://www.wikidata.org/entity/Q<span style="color: #8bc34a;">148</span> sample_storage_conditions: frozen specimen - source_database_accession: <span style="color: #2196F3;">[</span>http://identifiers.org/insdc/LC<span style="color: #8bc34a;">522350.1</span>#sequence] + source_database_accession: [http://identifiers.org/insdc/LC<span style="color: #8bc34a;">522350.1</span>#sequence] additional_collection_information: Optional free text field for additional information virus: @@ -565,28 +564,28 @@ virus: virus_strain: SARS-CoV-<span style="color: #8bc34a;">2</span>/human/CHN/HS_<span style="color: #8bc34a;">8</span>/<span style="color: #8bc34a;">2020</span> technology: - sample_sequencing_technology: <span style="color: #EF6C00;">[</span>http://www.ebi.ac.uk/efo/EFO_<span style="color: #8bc34a;">0009173</span>,http://www.ebi.ac.uk/efo/EFO_<span style="color: #8bc34a;">0009173</span>] + sample_sequencing_technology: [http://www.ebi.ac.uk/efo/EFO_<span style="color: #8bc34a;">0009173</span>,http://www.ebi.ac.uk/efo/EFO_<span style="color: #8bc34a;">0009173</span>] sequence_assembly_method: Protocol used for assembly - sequencing_coverage: <span style="color: #B388FF;">[</span><span style="color: #8bc34a;">70.0</span>, <span style="color: #8bc34a;">100.0</span><span style="color: #B388FF;">]</span> + sequencing_coverage: [<span style="color: #8bc34a;">70.0</span>, <span style="color: #8bc34a;">100.0</span>] additional_technology_information: Optional free text field for additional information submitter: - authors: <span style="color: #B388FF;">[</span>John Doe, Joe Boe, Jonny Oe<span style="color: #B388FF;">]</span> - submitter_name: <span style="color: #B388FF;">[</span>John Doe<span style="color: #B388FF;">]</span> + authors: [John Doe, Joe Boe, Jonny Oe] + submitter_name: [John Doe] submitter_address: John Doe's address originating_lab: John Doe kitchen lab_address: John Doe's address provider_sample_id: XXX<span style="color: #8bc34a;">1</span> submitter_sample_id: XXX<span style="color: #8bc34a;">2</span> publication: PMID<span style="color: #8bc34a;">00001113</span> - submitter_orcid: <span style="color: #B388FF;">[</span>https://orcid.org/<span style="color: #8bc34a;">0000</span>-<span style="color: #8bc34a;">0000</span>-<span style="color: #8bc34a;">0000</span>-<span style="color: #8bc34a;">0000</span>,https://orcid.org/<span style="color: #8bc34a;">0000</span>-<span style="color: #8bc34a;">0000</span>-<span style="color: #8bc34a;">0000</span>-<span style="color: #8bc34a;">0001</span>] + submitter_orcid: [https://orcid.org/<span style="color: #8bc34a;">0000</span>-<span style="color: #8bc34a;">0000</span>-<span style="color: #8bc34a;">0000</span>-<span style="color: #8bc34a;">0000</span>,https://orcid.org/<span style="color: #8bc34a;">0000</span>-<span style="color: #8bc34a;">0000</span>-<span style="color: #8bc34a;">0000</span>-<span style="color: #8bc34a;">0001</span>] additional_submitter_information: Optional free text field for additional information </pre> </div> </div> -<div id="outline-container-org37fadbc" class="outline-3"> -<h3 id="org37fadbc"><span class="section-number-3">6.1</span> Run the uploader (CLI)</h3> +<div id="outline-container-orge1c3625" class="outline-3"> +<h3 id="orge1c3625"><span class="section-number-3">6.1</span> Run the uploader (CLI)</h3> <div class="outline-text-3" id="text-6-1"> <p> Installing with pip you should be @@ -621,8 +620,8 @@ The web interface using this exact same script so it should just work </div> </div> -<div id="outline-container-org39adf09" class="outline-3"> -<h3 id="org39adf09"><span class="section-number-3">6.2</span> Example: uploading bulk GenBank sequences</h3> +<div id="outline-container-org07f04d9" class="outline-3"> +<h3 id="org07f04d9"><span class="section-number-3">6.2</span> Example: uploading bulk GenBank sequences</h3> <div class="outline-text-3" id="text-6-2"> <p> We also use above script to bulk upload GenBank sequences with a <a href="https://github.com/arvados/bh20-seq-resource/blob/master/scripts/download_genbank_data/from_genbank_to_fasta_and_yaml.py">FASTA @@ -634,7 +633,7 @@ took above for uploading a GenBank sequence are already automated. </div> </div> <div id="postamble" class="status"> -<hr><small>Created by <a href="http://thebird.nl/">Pjotr Prins</a> (pjotr.public768 at thebird 'dot' nl) using Emacs org-mode and a healthy dose of Lisp!<br />Modified 2020-05-30 Sat 18:12</small>. +<hr><small>Created by <a href="http://thebird.nl/">Pjotr Prins</a> (pjotr.public768 at thebird 'dot' nl) using Emacs org-mode and a healthy dose of Lisp!<br />Modified 2020-08-22 Sat 07:43</small>. </div> </body> </html> diff --git a/doc/blog/using-covid-19-pubseq-part3.org b/doc/blog/using-covid-19-pubseq-part3.org index e8fee36..b1ab90d 100644 --- a/doc/blog/using-covid-19-pubseq-part3.org +++ b/doc/blog/using-covid-19-pubseq-part3.org @@ -146,7 +146,6 @@ instead on https and entity instead of wiki) the submission went through. Reload the page (it won't empty the fields) to re-enable the submit button. - * Step 4: Check output The current pipeline takes 5.5 hours to complete! Once it completes diff --git a/doc/blog/using-covid-19-pubseq-part5.html b/doc/blog/using-covid-19-pubseq-part5.html index 4caa5ac..5d640f9 100644 --- a/doc/blog/using-covid-19-pubseq-part5.html +++ b/doc/blog/using-covid-19-pubseq-part5.html @@ -3,7 +3,7 @@ "http://www.w3.org/TR/xhtml1/DTD/xhtml1-strict.dtd"> <html xmlns="http://www.w3.org/1999/xhtml" lang="en" xml:lang="en"> <head> -<!-- 2020-07-17 Fri 05:03 --> +<!-- 2020-08-22 Sat 07:43 --> <meta http-equiv="Content-Type" content="text/html;charset=utf-8" /> <meta name="viewport" content="width=device-width, initial-scale=1" /> <title>COVID-19 PubSeq (part 4)</title> @@ -248,19 +248,28 @@ for the JavaScript code in this tag. <h2>Table of Contents</h2> <div id="text-table-of-contents"> <ul> -<li><a href="#org758b923">1. Modify Metadata</a></li> -<li><a href="#orgec32c13">2. What is the schema?</a></li> -<li><a href="#org2e487b2">3. How is the website generated?</a></li> -<li><a href="#orge4dfe84">4. Modifying the schema</a></li> -<li><a href="#org564a7a8">5. Adding fields to the form</a></li> -<li><a href="#org633781a">6. <span class="todo TODO">TODO</span> Testing the license fields</a></li> +<li><a href="#org935d151">1. Modify Metadata</a></li> +<li><a href="#orgfb70872">2. What is the schema?</a></li> +<li><a href="#orga76b489">3. How is the website generated?</a></li> +<li><a href="#org80bb905">4. Changing the license field</a> +<ul> +<li><a href="#org3689b60">4.1. Modifying the schema</a></li> +<li><a href="#org07e0c66">4.2. Adding fields to the form</a></li> +<li><a href="#org1cfb94a">4.3. <span class="todo TODO">TODO</span> Testing the license fields</a></li> +</ul> +</li> +<li><a href="#org88d4555">5. Changing GEO or location field</a> +<ul> +<li><a href="#org063bcfa">5.1. Relaxing the shex constraint</a></li> +</ul> +</li> </ul> </div> </div> -<div id="outline-container-org758b923" class="outline-2"> -<h2 id="org758b923"><span class="section-number-2">1</span> Modify Metadata</h2> +<div id="outline-container-org935d151" class="outline-2"> +<h2 id="org935d151"><span class="section-number-2">1</span> Modify Metadata</h2> <div class="outline-text-2" id="text-1"> <p> The public sequence resource uses multiple data formats listed on the @@ -268,8 +277,8 @@ The public sequence resource uses multiple data formats listed on the for RDF and semantic web/linked data ontologies. This technology allows for querying data in unprescribed ways - that is, you can formulate your own queries without dealing with a preset model of that -data (so typical of CSV files and SQL tables). Examples of exploring -data are listed <a href="http://covid19.genenetwork.org/blog?id=using-covid-19-pubseq-part1">here</a>. +data (which is how one has to approach CSV files and SQL +tables). Examples of exploring data are listed <a href="http://covid19.genenetwork.org/blog?id=using-covid-19-pubseq-part1">here</a>. </p> <p> @@ -280,8 +289,8 @@ understand that anyone, including you, can change that information! </div> </div> -<div id="outline-container-orgec32c13" class="outline-2"> -<h2 id="orgec32c13"><span class="section-number-2">2</span> What is the schema?</h2> +<div id="outline-container-orgfb70872" class="outline-2"> +<h2 id="orgfb70872"><span class="section-number-2">2</span> What is the schema?</h2> <div class="outline-text-2" id="text-2"> <p> The default metadata schema is listed <a href="https://github.com/arvados/bh20-seq-resource/blob/master/bh20sequploader/bh20seq-schema.yml">here</a>. @@ -289,8 +298,8 @@ The default metadata schema is listed <a href="https://github.com/arvados/bh20-s </div> </div> -<div id="outline-container-org2e487b2" class="outline-2"> -<h2 id="org2e487b2"><span class="section-number-2">3</span> How is the website generated?</h2> +<div id="outline-container-orga76b489" class="outline-2"> +<h2 id="orga76b489"><span class="section-number-2">3</span> How is the website generated?</h2> <div class="outline-text-2" id="text-3"> <p> Using the schema we use <a href="https://pypi.org/project/PyShEx/">pyshex</a> shex expressions and <a href="https://github.com/common-workflow-language/schema_salad">schema salad</a> to @@ -300,9 +309,13 @@ All from that one metadata schema. </div> </div> -<div id="outline-container-orge4dfe84" class="outline-2"> -<h2 id="orge4dfe84"><span class="section-number-2">4</span> Modifying the schema</h2> +<div id="outline-container-org80bb905" class="outline-2"> +<h2 id="org80bb905"><span class="section-number-2">4</span> Changing the license field</h2> <div class="outline-text-2" id="text-4"> +</div> +<div id="outline-container-org3689b60" class="outline-3"> +<h3 id="org3689b60"><span class="section-number-3">4.1</span> Modifying the schema</h3> +<div class="outline-text-3" id="text-4-1"> <p> One of the first things we want to do is to add a field for the data license. Initially we only supported CC-4.0 as a license, but @@ -380,25 +393,25 @@ So, we'll add it simply as a title field. Now the draft schema is type: record fields: license_type: - doc: License types as refined in https://wiki.creativecommons.org/images/d/d6/Ccrel-1.0.pdf + doc: License types as refined <span style="color: #fff59d;">in</span> https:<span style="color: #b0bec5;">//</span><span style="color: #b0bec5;">wiki.creativecommons.org/images/d/d6/Ccrel-1.0.pdf</span> type: string? jsonldPredicate: - _id: https://creativecommons.org/ns#License + _id: https:<span style="color: #b0bec5;">//</span><span style="color: #b0bec5;">creativecommons.org/ns#License</span> title: doc: Attribution title related to license type: string? jsonldPredicate: - _id: http://semanticscience.org/resource/SIO_001167 + _id: http:<span style="color: #b0bec5;">//</span><span style="color: #b0bec5;">semanticscience.org/resource/SIO_001167</span> attribution_url: doc: Attribution URL related to license type: string? jsonldPredicate: - _id: https://creativecommons.org/ns#Work + _id: https:<span style="color: #b0bec5;">//</span><span style="color: #b0bec5;">creativecommons.org/ns#Work</span> attribution_source: doc: Attribution source URL type: string? jsonldPredicate: - _id: https://creativecommons.org/ns#Work + _id: https:<span style="color: #b0bec5;">//</span><span style="color: #b0bec5;">creativecommons.org/ns#Work</span> </pre> </div> @@ -411,13 +424,13 @@ gitter channel and I merged it. </div> </div> -<div id="outline-container-org564a7a8" class="outline-2"> -<h2 id="org564a7a8"><span class="section-number-2">5</span> Adding fields to the form</h2> -<div class="outline-text-2" id="text-5"> +<div id="outline-container-org07e0c66" class="outline-3"> +<h3 id="org07e0c66"><span class="section-number-3">4.2</span> Adding fields to the form</h3> +<div class="outline-text-3" id="text-4-2"> <p> To add the new fields to the form we have to modify it a little. If we go to the upload form we need to add the license box. The schema is -loaded in <a href="https://github.com/arvados/bh20-seq-resource/blob/a0c8ebd57b875f265e8b0efec4abfaf892eb6c45/bh20simplewebuploader/main.py#L229">main.py</a> in the 'generate<sub>form</sub>' function. +loaded in <a href="https://github.com/arvados/bh20-seq-resource/blob/a0c8ebd57b875f265e8b0efec4abfaf892eb6c45/bh20simplewebuploader/main.py#L229">main.py</a> in the 'generate-form' function. </p> <p> @@ -453,12 +466,71 @@ field to be optional - a missing license assumes it is CC-BY-4.0. </div> </div> -<div id="outline-container-org633781a" class="outline-2"> -<h2 id="org633781a"><span class="section-number-2">6</span> <span class="todo TODO">TODO</span> Testing the license fields</h2> +<div id="outline-container-org1cfb94a" class="outline-3"> +<h3 id="org1cfb94a"><span class="section-number-3">4.3</span> <span class="todo TODO">TODO</span> Testing the license fields</h3> +</div> +</div> + +<div id="outline-container-org88d4555" class="outline-2"> +<h2 id="org88d4555"><span class="section-number-2">5</span> Changing GEO or location field</h2> +<div class="outline-text-2" id="text-5"> +<p> +When fetching information from GenBank and EBI/ENA we also translate +the location into an unambiguous identifier. We opted for the wikidata +tag. E.g. for New York city it is <a href="https://www.wikidata.org/wiki/Q60">https://www.wikidata.org/wiki/Q60</a> +and for New York state it is <a href="https://www.wikidata.org/wiki/Q1384">https://www.wikidata.org/wiki/Q1384</a>. If +everyone uses these metadata URIs it is easy to group when making +queries. Note that we should be using +<a href="http://www.wikidata.org/entity/Q60">http://www.wikidata.org/entity/Q60</a> in the dataset (http instead of +https and entitity instead of wiki). +</p> + +<p> +Unfortunately the main repositories of SARS-CoV-2 have variable +strings of text for location and/or GPS coordinates. For us to support +our schema we had to translate all options and this proves expensive. +</p> +</div> + +<div id="outline-container-org063bcfa" class="outline-3"> +<h3 id="org063bcfa"><span class="section-number-3">5.1</span> Relaxing the shex constraint</h3> +<div class="outline-text-3" id="text-5-1"> +<p> +So we decide to relax the enforcement of this type of metadata and to +allow for a free form string. +</p> + +<p> +The schema already used <a href="http://purl.obolibrary.org/obo/GAZ_00000448">http://purl.obolibrary.org/obo/GAZ_00000448</a> +which states: +</p> + +<div class="org-src-container"> +<pre class="src src-js">Class: geographic + location + Term IRI: http:<span style="color: #b0bec5;">//</span><span style="color: #b0bec5;">purl.obolibrary.org/obo/GAZ_00000448</span> +Definition: A reference to a place on + the Earth, by its name or by its geographical location. +</pre> +</div> + +<p> +and when you check count by location in the <a href="./demo">DEMO</a> it lists a free +format. +</p> + +<p> +So, why does the validation step balk when importing GenBank? +The problem was in the <a href="https://github.com/arvados/bh20-seq-resource/blob/46d4b7a3a31f6605f81d43ecd6651d60a5782364/bh20sequploader/bh20seq-shex.rdf#L39">shex check</a> for RDF generation. +Removing the wikidata requirement relaxed the imports with this +<a href="https://github.com/arvados/bh20-seq-resource/commit/f776816ee2b1af7ccc84afb494f68a81a51f5a76">patch</a>. +</p> +</div> +</div> </div> </div> <div id="postamble" class="status"> -<hr><small>Created by <a href="http://thebird.nl/">Pjotr Prins</a> (pjotr.public768 at thebird 'dot' nl) using Emacs org-mode and a healthy dose of Lisp!<br />Modified 2020-07-16 Thu 03:27</small>. +<hr><small>Created by <a href="http://thebird.nl/">Pjotr Prins</a> (pjotr.public768 at thebird 'dot' nl) using Emacs org-mode and a healthy dose of Lisp!<br />Modified 2020-08-22 Sat 07:42</small>. </div> </body> </html> diff --git a/doc/blog/using-covid-19-pubseq-part5.org b/doc/blog/using-covid-19-pubseq-part5.org index ec768ed..e260078 100644 --- a/doc/blog/using-covid-19-pubseq-part5.org +++ b/doc/blog/using-covid-19-pubseq-part5.org @@ -17,6 +17,7 @@ - [[#adding-fields-to-the-form][Adding fields to the form]] - [[#testing-the-license-fields][Testing the license fields]] - [[#changing-geo-or-location-field][Changing GEO or location field]] + - [[#relaxing-the-shex-constraint][Relaxing the shex constraint]] * Modify Metadata @@ -169,6 +170,8 @@ Unfortunately the main repositories of SARS-CoV-2 have variable strings of text for location and/or GPS coordinates. For us to support our schema we had to translate all options and this proves expensive. +** Relaxing the shex constraint + So we decide to relax the enforcement of this type of metadata and to allow for a free form string. @@ -188,4 +191,5 @@ format. So, why does the validation step balk when importing GenBank? The problem was in the [[https://github.com/arvados/bh20-seq-resource/blob/46d4b7a3a31f6605f81d43ecd6651d60a5782364/bh20sequploader/bh20seq-shex.rdf#L39][shex check]] for RDF generation. -Removing the wikidata requirement relaxed the imports. +Removing the wikidata requirement relaxed the imports with this +[[https://github.com/arvados/bh20-seq-resource/commit/f776816ee2b1af7ccc84afb494f68a81a51f5a76][patch]]. |