From 1e9a735e84a8e89099b1de2ec66c56669caf6cc8 Mon Sep 17 00:00:00 2001 From: AndreaGuarracino Date: Sat, 21 Nov 2020 23:06:05 +0100 Subject: added abPOA workflow; typos --- workflows/pangenome-generate/abpoa.cwl | 26 +++++ .../odgi-build-from-spoa-gfa.cwl | 29 ----- .../odgi-build-from-xpoa-gfa.cwl | 29 +++++ .../pangenome-generate_abpoa.cwl | 120 +++++++++++++++++++++ .../pangenome-generate/pangenome-generate_spoa.cwl | 2 +- 5 files changed, 176 insertions(+), 30 deletions(-) create mode 100644 workflows/pangenome-generate/abpoa.cwl delete mode 100644 workflows/pangenome-generate/odgi-build-from-spoa-gfa.cwl create mode 100644 workflows/pangenome-generate/odgi-build-from-xpoa-gfa.cwl create mode 100644 workflows/pangenome-generate/pangenome-generate_abpoa.cwl (limited to 'workflows/pangenome-generate') diff --git a/workflows/pangenome-generate/abpoa.cwl b/workflows/pangenome-generate/abpoa.cwl new file mode 100644 index 0000000..fa9f157 --- /dev/null +++ b/workflows/pangenome-generate/abpoa.cwl @@ -0,0 +1,26 @@ +cwlVersion: v1.1 +class: CommandLineTool +inputs: + readsFA: File + script: + type: File + default: {class: File, location: relabel-seqs.py} +outputs: + abpoaGFA: + type: stdout +requirements: + InlineJavascriptRequirement: {} +hints: + DockerRequirement: + dockerPull: "quay.io/biocontainers/abpoa:1.0.5--hed695b0_0" + ResourceRequirement: + coresMin: 1 + ramMin: $(15 * 1024) + outdirMin: $(Math.ceil(inputs.readsFA.size/(1024*1024*1024) + 20)) +baseCommand: abpoa +stdout: $(inputs.readsFA.nameroot).O0.gfa +arguments: [ + $(inputs.readsFA), + -r 3, + -O, '0' +] diff --git a/workflows/pangenome-generate/odgi-build-from-spoa-gfa.cwl b/workflows/pangenome-generate/odgi-build-from-spoa-gfa.cwl deleted file mode 100644 index eee4031..0000000 --- a/workflows/pangenome-generate/odgi-build-from-spoa-gfa.cwl +++ /dev/null @@ -1,29 +0,0 @@ -cwlVersion: v1.1 -class: CommandLineTool -inputs: - inputGFA: File -outputs: - odgiGraph: - type: File - outputBinding: - glob: $(inputs.inputGFA.nameroot).unchop.sorted.odgi -requirements: - InlineJavascriptRequirement: {} -hints: - DockerRequirement: - dockerPull: "odgi-bash-binutils:latest" - ResourceRequirement: - coresMin: 4 - ramMin: $(15 * 1024) - outdirMin: $(Math.ceil((inputs.inputGFA.size/(1024*1024*1024)+1) * 2)) - InitialWorkDirRequirement: - # Will fail if input file is not writable (odgi bug) - listing: - - entry: $(inputs.inputGFA) - writable: true -arguments: - - "sh" - - "-c" - - >- - odgi build -g '$(inputs.inputGFA.path)' -o - | odgi unchop -i - -o - | - odgi sort -i - -p s -o $(inputs.inputGFA.nameroot).unchop.sorted.odgi diff --git a/workflows/pangenome-generate/odgi-build-from-xpoa-gfa.cwl b/workflows/pangenome-generate/odgi-build-from-xpoa-gfa.cwl new file mode 100644 index 0000000..eee4031 --- /dev/null +++ b/workflows/pangenome-generate/odgi-build-from-xpoa-gfa.cwl @@ -0,0 +1,29 @@ +cwlVersion: v1.1 +class: CommandLineTool +inputs: + inputGFA: File +outputs: + odgiGraph: + type: File + outputBinding: + glob: $(inputs.inputGFA.nameroot).unchop.sorted.odgi +requirements: + InlineJavascriptRequirement: {} +hints: + DockerRequirement: + dockerPull: "odgi-bash-binutils:latest" + ResourceRequirement: + coresMin: 4 + ramMin: $(15 * 1024) + outdirMin: $(Math.ceil((inputs.inputGFA.size/(1024*1024*1024)+1) * 2)) + InitialWorkDirRequirement: + # Will fail if input file is not writable (odgi bug) + listing: + - entry: $(inputs.inputGFA) + writable: true +arguments: + - "sh" + - "-c" + - >- + odgi build -g '$(inputs.inputGFA.path)' -o - | odgi unchop -i - -o - | + odgi sort -i - -p s -o $(inputs.inputGFA.nameroot).unchop.sorted.odgi diff --git a/workflows/pangenome-generate/pangenome-generate_abpoa.cwl b/workflows/pangenome-generate/pangenome-generate_abpoa.cwl new file mode 100644 index 0000000..8f98dc6 --- /dev/null +++ b/workflows/pangenome-generate/pangenome-generate_abpoa.cwl @@ -0,0 +1,120 @@ +#!/usr/bin/env cwl-runner +cwlVersion: v1.1 +class: Workflow +requirements: + ScatterFeatureRequirement: {} + StepInputExpressionRequirement: {} +inputs: + seqs: File + metadata: File + bin_widths: + type: int[] + default: [ 1, 4, 16, 64, 256, 1000, 4000, 16000] + doc: width of each bin in basepairs along the graph vector + cells_per_file: + type: int + default: 100 + doc: Cells per file on component_segmentation + reversed_sort: + type: string + default: "true" +outputs: + odgiGraph: + type: File + outputSource: buildGraph/odgiGraph + odgiPNG: + type: File + outputSource: vizGraph/graph_image + abpoaGFA: + type: File + outputSource: induceGraph/abpoaGFA +# odgiRDF: +# type: File +# outputSource: odgi2rdf/rdf + readsMergeDedupSortedByQualAndLen: + type: File + outputSource: dedup_and_sort_by_quality_and_len/sortedReadsFA + mergedMetadata: + type: File + outputSource: dups2metadata/merged +# indexed_paths: +# type: File +# outputSource: index_paths/indexed_paths +# colinear_components: +# type: Directory +# outputSource: segment_components/colinear_components +steps: + dedup_and_sort_by_quality_and_len: + in: {readsFA: seqs, reversed_sorting: reversed_sort} + out: [sortedReadsFA, dups] + run: sort_fasta_by_quality_and_len.cwl + induceGraph: + in: + readsFA: dedup_and_sort_by_quality_and_len/sortedReadsFA + out: [abpoaGFA] + run: abpoa.cwl + buildGraph: + in: {inputGFA: induceGraph/abpoaGFA} + out: [odgiGraph] + run: odgi-build-from-xpoa-gfa.cwl + vizGraph: + in: + sparse_graph_index: buildGraph/odgiGraph + width: + default: 50000 + height: + default: 500 + path_per_row: + default: true + path_height: + default: 4 + out: [graph_image] + requirements: + ResourceRequirement: + ramMin: $(15 * 1024) + outdirMin: 10 + run: ../tools/odgi/odgi_viz.cwl + # odgi2rdf: + # in: {odgi: buildGraph/odgiGraph} + # out: [rdf] + # run: odgi_to_rdf.cwl + dups2metadata: + in: + metadata: metadata + dups: dedup_and_sort_by_quality_and_len/dups + out: [merged] + run: dups2metadata.cwl + # bin_paths: + # requirements: + # ResourceRequirement: + # ramMin: 3000 + # outdirMin: 10 + # run: ../tools/odgi/odgi_bin.cwl + # in: + # sparse_graph_index: buildGraph/odgiGraph + # bin_width: bin_widths + # scatter: bin_width + # out: [ bins, pangenome_sequence ] + # index_paths: + # label: Create path index + # requirements: + # ResourceRequirement: + # ramMin: 3000 + # outdirMin: 10 + # run: ../tools/odgi/odgi_pathindex.cwl + # in: + # sparse_graph_index: buildGraph/odgiGraph + # out: [ indexed_paths ] + # segment_components: + # label: Run component segmentation + # run: ../tools/graph-genome-segmentation/component_segmentation.cwl + # in: + # bins: bin_paths/bins + # cells_per_file: cells_per_file + # pangenome_sequence: + # source: bin_paths/pangenome_sequence + # valueFrom: $(self[0]) + # # the bin_paths step is scattered over the bin_width array, but always using the same sparse_graph_index + # # the pangenome_sequence that is extracted is exactly the same for the same sparse_graph_index + # # regardless of bin_width, so we take the first pangenome_sequence as input for this step + # out: [ colinear_components ] diff --git a/workflows/pangenome-generate/pangenome-generate_spoa.cwl b/workflows/pangenome-generate/pangenome-generate_spoa.cwl index 4e7302d..fa16809 100644 --- a/workflows/pangenome-generate/pangenome-generate_spoa.cwl +++ b/workflows/pangenome-generate/pangenome-generate_spoa.cwl @@ -56,7 +56,7 @@ steps: buildGraph: in: {inputGFA: induceGraph/spoaGFA} out: [odgiGraph] - run: odgi-build-from-spoa-gfa.cwl + run: odgi-build-from-xpoa-gfa.cwl vizGraph: in: sparse_graph_index: buildGraph/odgiGraph -- cgit v1.2.3